Find Research Outputs — Graz University of Technology

2018

l-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: Aeration and carbon source influence yields and productivities

Novy, V., Brunner, B. & Nidetzky, B., 11 Apr 2018, In : Microbial cell factories . 17, 1, 59.

Research output: Contribution to journal › Article

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

Valikhani, D., Bolivar, J. M., Dennig, A. & Nidetzky, B., 1 Oct 2018, In : Biotechnology and Bioengineering. 115, 10, p. 2416-2425 10 p.

Research output: Contribution to journal › Article

2016

Cover Image, Volume 113, Number 9, September 2016

Bolivar, J. M., Krämer, C. E., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering.

Research output: Contribution to journal › Article

2013

Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis, carrying a natural Ser-to-Thr substitution

Schmoelzer, K., Ribitsch, D., Czabany, T., Luley, C., Kokot, D., Lyskowski, A. F., Zitzenbacher, S., Schwab, H. & Nidetzky, B., 2013.

Research output: Contribution to conference › Poster

A newly discovered multifunctional α2,3-sialyltransferase from Pasteurella dagmatis PdST carries a natural Ser-to-Thr substitution

Schmoelzer, K., Ribitsch, D., Czabany, T., Luley, C., Kokot, D., Lyskowski, A. F., Zitzenbacher, S., Schwab, H. & Nidetzky, B., 10 Sep 2013.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2012

Catalytic mechanism of human UDP-glucose 6-dehydrogenase: in situ proton NMR studies reveal that the C-5 hydrogen of UDP-glucose is not exchanged with bulk water during the enzymatic reaction

Eixelsberger, T., Brecker, L. & Nidetzky, B., 2012, In : Carbohydrate Research. 356, p. 209-214

Research output: Contribution to journal › Article

2018

Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Escobar, S., Bernal, C., Bolivar, J. M., Nidetzky, B., López-Gallego, F. & Mesa, M., 1 Apr 2018, In : Molecular Catalysis. p. 106-113 8 p.

Research output: Contribution to journal › Article

2013

Switching between O- and C-glycosylation by active-site motif exchange in plant glycosyltransferases

Gutmann, A. & Nidetzky, B., 21 Apr 2013.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2017

Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l-lactate dehydrogenase within pdc1-pdc5 deletion background

Novy, V., Brunner, B., Müller, G. & Nidetzky, B., 1 Jan 2017, In : Biotechnology and Bioengineering. 114, 1, p. 163-171 9 p.

Research output: Contribution to journal › Article

2012

Switching between O‐and C‐Glycosyltransferase through Exchange of Active‐Site Motifs

Gutmann, A. & Nidetzky, B., 2012, In : Angewandte Chemie / International Edition . 51, 51, p. 12879-12883

Research output: Contribution to journal › Article

2013

Shine a light Shine a light on immobilized enzymes: real-time sensing in solid supported biocatalysts

Bolivar Bolivar, J. M., Consolati, T., Mayr, T. & Nidetzky, B., 2013, In : Trends in Biotechnology. 31, 3, p. 194-203

Research output: Contribution to journal › Article

2014

Surface structural dynamics of enzymatic cellulose degradation, revealed by combined kinetic and atomic force microscopy studies

Eibinger, M., Bubner, P., Ganner, T., Plank, H. & Nidetzky, B., 2014, In : The FEBS journal. 281, p. 275-290

Research output: Contribution to journal › Article

2013

AFM based Studies into Enzymatic Cellulose Degradation using a novel Substrate

Eibinger, M., Bubner, P., Ganner, T., Dohr, J., Mayrhofer, C., Plank, H. & Nidetzky, B., 2013.

Research output: Contribution to conference › Poster

2014

A two-step O- to C-glycosidic bond rearrangement using complementary glycosyltransferase activities

Gutmann, A., Krump, C., Bungaruang, L. & Nidetzky, B., 2014, In : Chemical Communications. 50, 41, p. 5465-5468

Research output: Contribution to journal › Article

2012

Stability of L-Lactic Dehydrogenase in a Lab-Scale Freezing Container

Rößl, U., Jajcevic, D., Leitgeb, S. & Nidetzky, B., 2012.

Research output: Contribution to conference › Poster

Ein Motiv im aktiven Zentrum fungiert als Schalter zwischen O‐und C‐Glykosyltransferase‐Aktivität

Gutmann, A. & Nidetzky, B., 2012, In : Angewandte Chemie. 124, 51, p. 13051-13056

Research output: Contribution to journal › Article

2016

Kinetic analysis and probing with substrate analogues of the reaction pathway of the nitrile reductase QueF from Escherichia coli

Jung, J., Czabany, T., Wilding, B., Klempier, N. & Nidetzky, B., 2016, In : The Journal of Biological Chemistry. 291, 49, p. 25411-25426 16 p.

Research output: Contribution to journal › Article

2013

Characterization of a Laboratory-Scale Container for Freezing Protein Solutions with Detailed Evaluation of a Freezing Process Simulation

Rößl, U., Jajcevic, D., Leitgeb, S., Khinast, J. & Nidetzky, B., 2013, In : Journal of Pharmaceutical Sciences . 103, 2, p. 417-426

Research output: Contribution to journal › Article

2016

The micromorphology of Trichoderma reesei analyzed in cultivations on lactose and solid lignocellulosic substrate, and its relationship with cellulase production

Novy, V., Schmid, M., Eibinger, M., Petrasek, Z. & Nidetzky, B., 9 Aug 2016, In : Biotechnology for Biofuels. 9, 1, 169.

Research output: Contribution to journal › Article

Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

Schmoelzer, K., Gutmann, A., Diricks, M., Desmet, T. & Nidetzky, B., 2016, In : Biotechnology Advances. 34, 2, p. 88-111

Research output: Contribution to journal › Article

2014

Enzymatic redox cascade for synthesis of nucleotide sugars

Eixelsberger, T., Horvat, D. & Nidetzky, B., 2014.

Research output: Contribution to conference › Poster

2012

Investigating Freeze and Thaw Behavior of Protein Solutions using a Lab-Scale Freezing Container

Rößl, U., Jajcevic, D., Leitgeb, S. & Nidetzky, B., 4 May 2012.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2014

Multi-enzymatic synthesis of UDP-xylose

Eixelsberger, T. & Nidetzky, B., 13 Feb 2014.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2016

Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1

Dewitte, G., Walmagh, M., Diricks, M., Lepak, A., Gutmann, A., Nidetzky, B. & Desmet, T., 1 Jun 2016, In : Journal of Biotechnology. 233, p. 49-55

Research output: Contribution to journal › Article

2012

Employing disulphide bond mapping to investigate the aggregation mechanism of rhG-CSF

Rößl, U., Wiesbauer, J., Leitgeb, S., Birner-Grünberger, R. & Nidetzky, B., 2012.

Research output: Contribution to conference › Poster

2014

Glycosyltransferases: On the way towards industrial biocatalysts

Schmoelzer, K., Gutmann, A. & Nidetzky, B., 16 Dec 2014.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2018

Process intensification for O₂-dependent enzymatic transformations in continuous single-phase pressurized flow

Bolivar, J. M., Mannsberger, A., Thomsen, M. S., Tekautz, G. & Nidetzky, B., 4 Dec 2018, In : Biotechnology and Bioengineering.

Research output: Contribution to journal › Article

2014

Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis, carrying a natural Ser-to-Thr substitution

Schmoelzer, K., Ribitsch, D., Czabany, T., Luley, C., Kokot, D., Lyskowski, A. F., Zitzenbacher, S., Schwab, H. & Nidetzky, B., 2014.

Research output: Contribution to conference › Poster

Convenient microtiter plate-based, oxygen-independent activity assays for flavin-dependent oxidoreductases based on different redox dyes

Brugger, D., Krondorfer, I., Zahma, K., Stoisser, T., Bolivar, J. M., Nidetzky, B., Peterbauer, C. K. & Haltrich, D., 2014, In : Biotechnology Journal. 9, 4, p. 474-482 9 p.

Research output: Contribution to journal › Article

2012

Aggregation of hGH - Insights by accelerated stress studies and reversible refolding

Wiesbauer, J., Boeszoermenyi, A. & Nidetzky, B., 2012.

Research output: Contribution to conference › Poster

2018

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

Jung, J. & Nidetzky, B., 1 Jan 2018, In : Journal of Biological Chemistry. 293, 10, p. 3720-3733 14 p.

Research output: Contribution to journal › Article

2013

Characterization of a multifunctional α2,3-sialyltransferase from Pasteurella dagmatis

Schmoelzer, K., Ribitsch, D., Czabany, T., Luley, C., Kokot, D., Lyskowski, A. F., Zitzenbacher, S., Schwab, H. & Nidetzky, B., 2013, In : Glycobiology. 23, 11, p. 1293-1304

Research output: Contribution to journal › Article

2014

High-quality production of human α-2,6-sialyltransferase in Pichia pastoris requires control over N-terminal truncations by host-inherent protease activities

Ribitsch, D., Zitzenbacher, S., Augustin, P. A., Schmoelzer, K., Luley, C., Thomann, M., Jung, C., Sobek, H., Müller, R., Nidetzky, B. & Schwab, H., 2014, In : Microbial cell factories . 13, p. 138-149

Research output: Contribution to journal › Article

2016

Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems.

Petschacher, B. & Nidetzky, B., 1 Apr 2016, In : Journal of Biotechnology. 235, p. 61-83 23 p.

Research output: Contribution to journal › Article

2017

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

Research output: Contribution to journal › Article

2012

Structure and mechanism of human UDP-xylose synthase

Eixelsberger, T., Sykora, S., Egger, S., Brunsteiner, M., Kavanagh, K., Oppermann, U., Brecker, L. & Nidetzky, B., 21 May 2012.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2017

Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie / International Edition . 56, p. 2503 –2507

Research output: Contribution to journal › Article

2012

Structure and mechanism of human UDP-xylose synthase: evidence for a promoting role of sugar ring distortion in a three-step catalytic conversion of UDP-glucuronic acid

Eixelsberger, T., Sykora, S., Egger, S., Brunsteiner, M., Kavanagh, K., Oppermann, U., Brecker, L. & Nidetzky, B., 2012, In : The Journal of Biological Chemistry. 287, 37, p. 31349-31358

Research output: Contribution to journal › Article

2020

A Biological Nanomachine at Work: Watching the Cellulosome Degrade Crystalline Cellulose

Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 27 May 2020, In : ACS Central Science. 6, 5, p. 739-746 8 p.

Research output: Contribution to journal › Article

Open Access

2012

Structural and kinetic evidence that the catalytic reaction of human UDP-glucose 6-dehydrogenase involves covalent thiohemiacetal and thioester enzyme intermediates

Egger, S., Chaikuad, A., Klimacek, M., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 2012, In : The Journal of Biological Chemistry. 287, p. 2119-2129

Research output: Contribution to journal › Article

2014

Stepwise metabolic adaption from pure metabolization to balanced anaerobic growth on xylose explored for recombinant Saccharomyces cerevisiae

Klimacek, M., Kirl, E., Krahulec, S., Longus, K., Novy, V. & Nidetzky, B., 2014, In : Microbial cell factories . 13, 37, p. 1-12

Research output: Contribution to journal › Article

2013

From NMR to enzymatic information

Vogl, M., Kratzer, R., Nidetzky, B. & Brecker, L., 21 Apr 2013.

Research output: Contribution to conference › (Old data) Lecture or Presentation

2020

On the relationship between structure and catalytic effectiveness in solid surface-immobilized enzymes: Advances in methodology and the quest for a single-molecule perspective

Bolivar, J. M. & Nidetzky, B., 1 Feb 2020, In : Biochimica et Biophysica Acta - Proteins and Proteomics. 1868, 2, 140333.

Research output: Contribution to journal › Review article

On the donor substrate dependence of group‐transfer reactions by hydrolytic enzymes: Insight from kinetic analysis of sucrose phosphorylase‐catalyzed transglycosylation

Klimacek, M., Sigg, A. & Nidetzky, B., 23 Jun 2020, In : Biotechnology and Bioengineering. 2020, 117

Research output: Contribution to journal › Article

Enzyme Immobilization in Wall-Coated Flow Microreactors

Valikhani, D., Bolivar, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 243-257 15 p.

Research output: Contribution to journal › Article

Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisan, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 319-333 15 p.

Research output: Contribution to journal › Article

2013

Biological nitrile reduction – exploring a remarkable reaction

Wilding, B., Petschacher, B., Winkler, M., Kratzer, R., Steinkellner, G., Lyskowski, A. F., Gruber, K., Nidetzky, B. & Klempier, N., 23 Sep 2013.

Research output: Contribution to conference › (Old data) Lecture or Presentation

Freezing Process Development under QbD Principles - In-silico Solutions for Process Characterization and Scaling

Rößl, U., Leitgeb, S., Jajcevic, D. & Nidetzky, B., 2013.

Research output: Contribution to conference › Poster

Smart enzyme immobilization in microstructured reactors

Bolivar, J. M. & Nidetzky, B., May 2013, In : Chimica Oggi. 31, 3, p. 50-54 5 p.

Research output: Contribution to journal › Article

Shine a light on immobilized enzymes: Real-time sensing in solid supported biocatalysts

Bolivar, J. M., Consolati, T., Mayr, T. & Nidetzky, B., Mar 2013, In : Trends in Biotechnology. 31, 3, p. 194-203 10 p.

Research output: Contribution to journal › Article

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