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• 2020

  Engineering of Saccharomyces cerevisiae for the production of (+)-ambrein

  Moser, S., Leitner, E., Plocek, T. J., Vanhessche, K. & Pichler, H., 14 Jan 2020, In : Yeast. 37, 1, p. 163 - 172 10 p.

  Research output: Contribution to journal › Article

  Open Access

  • Yeast
  • Saccharomyces cerevisiae
  • Biosynthesis
  • Squalene
  • Fragrances

• Secretion of Pseudomonas Aeruginosa Lipoxygenase by Pichia Pastoris upon Glycerol Feed

  Hashem, C., Stolterfoht, H., Rinnofner, C., Steinberger, S., Winkler, M. & Pichler, H., Nov 2020, In : Biotechnology Journal. 15, 11, 2000089.

  Research output: Contribution to journal › Article

  Open Access

  • Lipoxygenase
  • Pichia
  • Glycerol
  • Pseudomonas aeruginosa
  • Methanol
• 2019

  Application of hydrates in industrial biocatalysts

  Freudenthaler, J., Engleder, M., Emmerstorfer-Augustin, A., Wriessnegger, T., Pavkov-Keller, T., Steinkellner, G., Leitner, E., Strohmeier, G., Kaluzna, I., Müller, M., Mink, D., Schürmann, M., Pichler, H. & Gruber, K., 18 Nov 2019.

  Research output: Contribution to conference › Poster

• Evolving the Promiscuity of Elizabethkingia meningoseptica Oleate Hydratase for the Regio- and Stereoselective Hydration of Oleic Acid Derivatives

  Engleder, M., Strohmeier, G. A., Weber, H., Steinkellner, G., Leitner, E., Müller, M., Mink, D., Schürmann, M., Gruber, K. & Pichler, H., 27 May 2019, In : Angewandte Chemie - International Edition. 58, 22, p. 7480-7484 5 p.

  Research output: Contribution to journal › Article

  Open Access

  • Hydro-Lyases
  • Oleic acid
  • Oleic Acid
  • Fatty acids
  • Hydration

• Exploring castellaniella defragrans linalool (De)hydratase-isomerase for enzymatic hydration of alkenes

  Engleder, M., Müller, M., Kaluzna, I., Mink, D., Schürmann, M., Leitner, E., Pichler, H. & Emmerstorfer-Augustin, A., 1 Jun 2019, In : Molecules . 24, 11, 2092.

  Research output: Contribution to journal › Article

  Open Access

  • Hydro-Lyases
  • Isomerases
  • Alkenes
  • Hydration
  • alkenes

• From Fatty Acid to Green Leaf Volatiles by recombinant Lipoxygenases (LOX) and Hydroperoxide Lyases (HPL)

  Steinberger, S., Hashem, C., Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., Nov 2019.

  Research output: Contribution to conference › Poster

• Goodbye methanol induction: Secretion of Pseudomonas aeruginosa lipoxygenase by Pichia pastoris upon glycerol feed

  Hashem, C., Stolterfoht, H., Rinnofner, C., Steinberger, S., Winkler, M. & Pichler, H., Nov 2019.

  Research output: Contribution to conference › Poster

• Identifying and engineering the ideal microbial terpenoid production host

  Moser, S. & Pichler, H., 20 Jul 2019, In : Applied Microbiology and Biotechnology. 103, 14, p. 5501-5516 16 p.

  Research output: Contribution to journal › Review article

  Open Access

  • Terpenes
  • Cell Engineering
  • Biofuels
  • Saccharomyces cerevisiae
  • Carbon

• Membrane protein production in yeast: Modification of yeast membranes for human membrane protein production

  Emmerstorfer-Augustin, A., Wriessnegger, T., Hirz, M., Zellnig, G. & Pichler, H., 9 Feb 2019, Recombinant Protein Production in Yeast: Methods and Protocols. Springer, (Methods in Molecular Biology).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Modified Sterol acyltransferases

  Lehmann, M., Pichler, H. & Stolterfoht, H., 28 Nov 2019, IPC No. C12N 9/10 (2006.01) , C12P 33/00 (2006.01), C12N 15/52 (2006.01), C12P 1/00 (2006.01), WIPO, Patent No. PCT/EP20197063078, 21 May 2019, Priority date 22 May 2020, Priority No. 00628/18

  Research output: Patent

• Pichia pastoris protease-deficient and auxotrophic strains generated by a novel, user-friendly vector toolbox for gene deletion

  Ahmad, M., Winkler, C. M., Kolmbauer, M., Pichler, H., Schwab, H. & Emmerstorfer-Augustin, A., 1 Sep 2019, In : Yeast. 36, 9, p. 557-570 14 p.

  Research output: Contribution to journal › Article

  Open Access

  • Pichia
  • Gene Deletion
  • Peptide Hydrolases
  • Genes
  • Phenylalanine

• Recombinant (+)-ambrein production: Saccharomyces cerevisiae vs. Pichia pastoris

  Moser, S., Schneider, J., Strohmeier, G., Leitner, E., Plocek, T. J., Vanhessche, K. & Pichler, H., 18 Nov 2019.

  Research output: Contribution to conference › Poster

• Recombinant lipoxygenases and hydroperoxide lyases for the synthesis of green leaf volatiles

  Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., Oct 2019, In : Journal of Agricultural and Food Chemistry. 67, 49, p. 13367-13392 59 p.

  Research output: Contribution to journal › Review article

  • Lipoxygenases
  • lyases
  • hydroperoxides
  • lipoxygenase
  • synthesis

• Selective hydration of oleic acid derivatives

  Pichler, H., Schuermann, M., Schuetz, R., Engleder, M. & Strohmeier, G., 12 Dec 2019, IPC No. C11C 1/04 (2006.01), WIPO, Patent No. WO 2019/234043 A1, 4 Jun 2019, Priority date 4 Jun 2018, Priority No. 00706/18

  Research output: Patent

• The Production of Matchout-Deuterated Cholesterol and the Study of Bilayer-Cholesterol Interactions

  Waldie, S., Moulin, M., Porcar, L., Pichler, H., Strohmeier, G. A., Skoda, M., Forsyth, V. T., Haertlein, M., Maric, S. & Cárdenas, M., 1 Dec 2019, In : Scientific Reports. 9, 1, 5118.

  Research output: Contribution to journal › Article

  Open Access

  • Neutrons
  • Cholesterol
  • Small Angle Scattering
  • Lipids
  • Phosphatidylcholines

• Valorization of Fatty Acids by Pichia pastoris: A Fresh Approach for Green Leaf Volatile Synthesis

  Hashem, C., Steinberger, S., Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., 12 Nov 2019.

  Research output: Contribution to conference › Poster
• 2018

  Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine

  Waldie, S., Lind, T. K., Browning, K., Mouline, M., Haertlein, M., Forsyth, T., Luchini, A., Strohmeier, G., Pichler, H., Maric, S. & Cárdenas, M., 15 Feb 2018, In : Langmuir. 34, 1, p. 472-479 8 p.

  Research output: Contribution to journal › Article

  • Lipid bilayers
  • Cholesterol
  • cholesterol
  • Lipid Bilayers
  • Phosphatidylcholines

• Modification of Membrane Lipid Compositions in single-celled organisms–from Basics to Applications

  Pichler, H. & Emmerstorfer-Augustin, A., 13 Sep 2018, In : Methods. 147, p. 50 - 65 16 p.

  Research output: Contribution to journal › Article

  Open Access

• On the current role of hydratases in biocatalysis

  Engleder, M. & Pichler, H., 15 Jul 2018, In : Applied Microbiology and Biotechnology. 102, p. 5841-5858

  Research output: Contribution to journal › Article

  Open Access

• Perdeuteration of cholesterol for neutron scattering applicatioins using recombinant Pichia pastoris

  Moulin, M., Strohmeier, G., Hirz, M., Thompson, K., Rennie, A., Campbell, R. A., Pichler, H., Maric, S., Forsyth, T. & Haertlein, M., 5 Feb 2018, In : Chemistry and Physics of Lipids. 212, p. 80-87

  Research output: Contribution to journal › Article

• Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca

  Engleder, M., Horvat, M., Emmerstorfer-Augustin, A., Wriessnegger, T., Gabriel, S., Strohmeier, G., Weber, H., Müller, M., Kaluzna, I., Mink, D., Schürmann, M. & Pichler, H., 8 Feb 2018, In : PLoS ONE.

  Research output: Contribution to journal › Article

  Open Access

• The Impact of Deuteration on Natural and Synthetic Lipids: a Neutron Diffraction Study

  Luchini, A., Delhom, R., Demé, B., Laux, V., Mouline, M., Haertlein, M., Pichler, H., Strohmeier, G., Wacklin, H. & Fragneto, G., 1 Aug 2018, In : Colloids and Surfaces / B. p. 126 - 133

  Research output: Contribution to journal › Article

• Whole-cell (+)-ambrein production in the yeast Pichia pastoris

  Moser, S., Strohmeier, G., Leitner, E., Plocek, T. J., Vanhessche, K. & Pichler, H., Dec 2018, In : Metabolic Engineering Communications. 7, 9 p., e00077.

  Research output: Contribution to journal › Article

  Open Access

• Whole-cell (+)-ambrein production in the yeast Pichia pastoris

  Moser, S., Strohmeier, G. A., Leitner, E., Plocek, T. J., Vanhessche, K. & Pichler, H., 26 Jun 2018.

  Research output: Contribution to conference › Poster
• 2017

  Application of hydratases for flavor and fragrance synthesis

  Engleder, M., Pavkov-Keller, T., Emmerstorfer, A., Hromic, A., Schrempf, S., Steinkellner, G., Wriessnegger, T., Leitner, E., Strohmeier, G., Kaluzna, I., Daniel, M., Schürmann, M., Wallner, S., Macheroux, P., Pichler, H. & Gruber, K., 14 Nov 2017.

  Research output: Contribution to conference › Poster

• Application of hydratases for flavour and fragrance synthesis

  Engleder, M., Pavkov-Keller, T., Emmerstorfer, A., Altijana, H., Schrempf, S., Steinkellner, G., Wriessnegger, T., Leitner, E., Strohmeier, G., Kaluzna, I., Mink, D., Schürmann, M., Wallner, S., Macheroux, P., Gruber, K. & Pichler, H., 20 Sep 2017.

  Research output: Contribution to conference › Poster

• Carboxylic Acid Reductase mediated Biotransformations

  Schwendenwein, D., Fiume, G., Pichler, H. & Winkler, M., 11 Jul 2017.

  Research output: Contribution to conference › Poster

• Enhancing (+)-nootkatone production in P. pastoris and S. cerevisiae through CYP450-mediated conversions

  Moser, S., Wriessnegger, T., Emmerstorfer-Augustin, A., Wimmer-Teubenbacher, M., Leitner, E., Engleder, M., Müller, M., Daniel, M., Schurmann, M. & Pichler, H., 14 Nov 2017.

  Research output: Contribution to conference › Poster

• Enhancing (+)-nootkatone production in Pichia pastoris and Saccharomyces cerevisiae through cytochrome P450-mediated conversions

  Moser, S., Wriessnegger, T., Emmerstorfer-Augustin, A., Wimmer-Teubenbacher, M., Leitner, E., Engleder, M., Müller, M., Schürmann, M., Pichler, H. & Daniel, M., 20 Sep 2017.

  Research output: Contribution to conference › Poster

• Enhancing cytochrome P450-mediated conversions in P. pastoris

  Moser, S., Wriessnegger, T., Emmerstorfer-Augustin, A., Leitner, E., Müller, M., Kaluzna, I., Schürmann, M., Daniel, M. & Pichler, H., 24 Apr 2017.

  Research output: Contribution to conference › Poster

• Identification of key residues in Neurospora crassa Carboxylate reductase (CAR)

  Stolterfoht, H., Schwendenwein, D., Rudroff, F., Pichler, H. & Winkler, M., 15 Nov 2017.

  Research output: Contribution to conference › Poster

• Identification of key residues in Neurospora crassa Carboxylate reductase (CAR)

  Stolterfoht, H., Schwendenwein, D., Rudroff, F., Pichler, H. & Winkler, M., 11 Jul 2017.

  Research output: Contribution to conference › Poster

• Production of sterols in modified yeasts

  Hohmann, H-P., Leber, R., Lehmann, M., Odar, C., Petschacher, B. & Pichler, H., 29 Jun 2017, IPC No. C12P/33/00, Patent No. PCT/EP2016/, Priority date 23 Dec 2015, Priority No. 01908/15

  Research output: Patent

• Structure-based reaction mechanism of oleate hydratase from Elizabethkingia meningoseptica

  Engleder, M., Pavkov-Keller, T., Emmerstorfer-Augustin, A., Hromic, A., Schrempf, S., Steinkellner, G., Wriessnegger, T., Leitner, E., Strohmeier, G., Kaluzna, I., Daniel, M., Martin, S., Wallner, S., Macheroux, P., Gruber, K. & Pichler, H., 23 Apr 2017.

  Research output: Contribution to conference › Poster

  • Hydro-Lyases
  • Oleic Acid
  • Hydration
  • Flavin-Adenine Dinucleotide
  • Carbon
• 2016

  Enhancing cytochrome P450-mediated conversions in P. pastoris

  Moser, S., Wriessnegger, T., Emmerstorfer, A., Leitner, E., Müller, M., Kaluzna, I., Schurmann, M., Daniel, M. & Pichler, H., 15 Nov 2016.

  Research output: Contribution to conference › Poster

• Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions

  Wriessnegger, T., Moser, S., Emmerstorfer-Augustin, A., Leitner, E., Müller, M., Kaluzna, I., Schürmann, M., Daniel, M. & Pichler, H., 8 Mar 2016, In : Fungal genetics and biology. 89, p. 114-125

  Research output: Contribution to journal › Article

• Metabolic engineering of Pichia pastoris for ginsenoside production

  Emmerstorfer-Augustin, A., Odar, C., Wriessnegger, T. & Pichler, H., 15 Nov 2016.

  Research output: Contribution to conference › Poster

• Oleate hydratases - structure function relationship and application

  Pichler, H., 1 Oct 2016.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Oleate hydratase - structure function relationships and application

  Pichler, H., 1 Oct 2016, Power of microbes in industry and environment: Book of abstracts. Mrsa, V., Teparic, R. & Kifer, D. (eds.). Zagreb: Recedo d.o.o., Zagreb, p. 33 1 p.

  Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

• Optimization of Peptide Secretion in P. pastoris

  Hirz, M., Odar, C., Leithold, C., Pichler, H. & Schwab, H., 5 Feb 2016.

  Research output: Contribution to conference › Poster

• Production of aromatic plant terpenoids in recombinant baker´s yeast

  Emmerstorfer, A. & Pichler, H., 2016, Biotechnology of Plant Secondary Metabolism. 1 ed. New York: Springer, p. 79-89 (Methods in Molecular Biology).

  Research output: Chapter in Book/Report/Conference proceeding › Chapter

• Screening for improved isoprenoid biosynthesis in microorganisms

  Emmerstorfer, A., Moser, S. & Pichler, H., 2016, In : Journal of Biotechnology. 235, p. 112–120

  Research output: Contribution to journal › Article

• Sterol and terpenoid engineering in yeasts

  Pichler, H., 10 May 2016.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Sterol-modified P. pastoris: Membrane Protein Expression and Cell Physiology

  Hirz, M., Richter, G., Wriessnegger, T., Zellnig, G., Leitner, E. & Pichler, H., 11 Jul 2016.

  Research output: Contribution to conference › Poster

• Structure-based reaction mechanism of oleate hydratase from Elizabethkingia meningoseptica

  Engleder, M., Pavkov-Keller, T., Emmerstorfer-Augustin, A., Altijana, H., Schrempf, S., Steinkellner, G., Wriessnegger, T., Leitner, E., Strohmeier, G., Kaluzna, I., Daniel, M., Schurmann, M., Wallner, S., Macheroux, P., Gruber, K. & Pichler, H., 15 Nov 2016.

  Research output: Contribution to conference › Poster
• 2015

  APPLYING CYTOCHROME P450 ENZYMES FOR FLAVOUR&FRAGRANCE BIOSYNTHESIS IN YEASTS

  Wriessnegger, T., Emmerstorfer, A., Moser, S., Wimmer-Teubenbacher, M., Augustin, P. A., Engleder, M., Leitner, E., Zellnig, G., Müller, M., Kaluzna, I., Mink, D., Schürmann, M., Schwab, H. & Pichler, H., 26 Jul 2015.

  Research output: Contribution to conference › (Old data) Lecture or Presentation

• Biotechnology of Cellular Membranes - Biosynthesis, Interactions and Functions of Hydrophobic Compounds

  Pichler, H., 2015

  Research output: Thesis › Habilitation

• ON THE REACTION MECHANISM OF THE ELIZABETHKINGIA MENINGOSEPTICA OLEATE HYDRATASE

  Engleder, M., Pavkov-Keller, T., Emmerstorfer, A., Hromic, A., Schrempf, S., Steinkellner, G., Wriessnegger, T., Leitner, E., Strohmeier, G., Kaluzna, I., Mink, D., Schürmann, M., Wallner, S., Macheroux, P., Gruber, K. & Pichler, H., 2015.

  Research output: Contribution to conference › Poster

• Over-expression of ICE2 stabilizes cytochrome P450 reductase levels in Saccharomyces cerevisiae and Pichia pastoris

  Emmerstorfer, A., Wimmer-Teubenbacher, M., Wriessnegger, T., Leitner, E., Müller, M., Kaluzna, I., Schürmann, M., Mink, D., Zellnig, G., Schwab, H. & Pichler, H., 2015, In : Biotechnology Journal. 10, p. 623-635

  Research output: Contribution to journal › Article

• Sphingolipids regulate telomere clustering by affecting transcriptional levels of genes involved in telomere homeostasis

  Ikeda, A., Muneoka, T., Murakami, S., Hirota, A., Yabuki, Y., Karashima, T., Nakazono, K., Tsuruno, M., Pichler, H., Shirahige, K., Kodama, Y., Shimamoto, T., Mizuta, K. & Funato, K., 2015, In : Journal of cell science. 128, p. 2454-2467

  Research output: Contribution to journal › Article