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2015

(R)-selective hydroxynitrile lyase variants with a cupin fold having improved substrate scope and the use thereof

WIEDNER, ROMANA., STEINER, KERSTIN., Glieder, A. & SCHWAB, HELMUT., 16 Dec 2015, IPC No. C12P 13/ 00 A I, Patent No. EP2955225, Priority date 12 Jun 2014, Priority No. EP20140172216

Research output: PatentResearch

lyases
amino acid substitution

An updated view on horseradish peroxidases: recombinant production and biotechnological applications.

Krainer, F. & Glieder, A., 2015, In : Applied Microbiology and Biotechnology. 99, 4, p. 1611-1625

Research output: Contribution to journalArticleResearchpeer-review

A toolbox of endogenous and heterologous nuclear localization sequences for the methylotrophic yeast Pichia pastoris

Weninger, A., Glieder, A. & Vogl, T., Nov 2015, In : FEMS yeast research. 15, 7, 4 p., fov082.

Research output: Contribution to journalArticleResearchpeer-review

Pichia
Yeasts
Proteins
Eukaryota
CRISPR-Associated Proteins

Bioprospecting for Hydroxynitrile Lyases by Blue Native PAGE Coupled HCN Detection

Lanfranchi, E., Köhler, E-M., Darnhofer, B., Steiner, K., Birner-Grünberger, R., Glieder, A. & Winkler, M., 2015, In : Current biotechnology. 4, 2, p. 111-117

Research output: Contribution to journalArticleResearchpeer-review

Biotransformations for drug metabolite synthesis

Winkler, M., Bachler, T., Geier, M., Hanlon, S. P., Eggimann, F. K., Rodrigues, D., Ferreira Antunes, M., Kittelmann, M., Glieder, A., Wirz, B. & Lütz, S., 2015.

Research output: Contribution to conferencePosterResearch

Compact multi-enzyme pathways in P. pastoris

Geier, M., Fauland, P. C., Vogl, T. & Glieder, A., 2015, In : Chemical Communications. 15, 51;9, p. 1643-1646

Research output: Contribution to journalArticleResearchpeer-review

Cyanohydrin Formation / Henry Reaction

Steiner, K., Glieder, A. & Gruber, M., 2015, Sience of Synthesis. 1 ed. Stuttgart: Thieme, p. 1-5

Research output: Chapter in Book/Report/Conference proceedingChapterResearch

Drug Metabolizing Enzymes: From Heterologous Expression to Drug Metabolite Synthesis

Winkler, M., Bachler, T., Geier, M., Glieder, A., Hanlon, S. P., Eggimann, F. K., Rodrigues, D., Antunes, M., Kittelmann, M., Wirz, B. & Lütz, S., 2015.

Research output: Contribution to conferencePosterResearch

Engineering Pichia pastoris for improved NADH regeneration: A novel chassis strain for whole-cell catalysis

Geier, M., Brandner, C., Strohmeier, G. A., Hall, M., Hartner, F. S. & Glieder, A., 2015, In : Beilstein Journal of Organic Chemistry . 11, p. 1741-8 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Chassis
NAD
Catalysis
Methanol
formaldehyde transketolase

Hydroxynitrile lyase from fern: A Unique Biocatalyst

Lanfranchi, E., Winkler, M., Pavkov-Keller, T., Köhler, E-M., Diepold, M., Darnhofer, B., Steiner, K., Thallinger, G., Birner-Grünberger, R., Van Pelt, S., Gruber, K. & Glieder, A., 21 Sep 2015.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Non-homologous enzyme discovery: the hydroxynitrile lyases case

Lanfranchi, E., Steiner, K., Darnhofer, B., Birner-Grünberger, R., Winkler, M. & Glieder, A., 20 Apr 2015.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Novel DNA and RNA elements

Pitzer, J., Van Hove, B., Love, A. M., Ajikumar, P. K., De Mey, M. & Glieder, A., 1 Jan 2015, Synthetic Biology. Cham: Springer International Publishing AG , p. 65-99 35 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

RNA
DNA
Riboswitch
Synthetic Biology
Walkers

Optimizing cofactor availability for the production of recombinant heme peroxidase in Pichia pastoris.

Krainer, F., Capone, S., Jäger, M., Vogl, T., Gerstmann, M. A., Glieder, A., Herwig, C. & Spadiut, O., 2015, In : Microbial cell factories . 14, 4, p. 1-9

Research output: Contribution to journalArticleResearchpeer-review

Preface

Glieder, A. & Kubicek, C. P., 1 Jan 2015, Synthetic Biology. Springer International Publishing AG , p. v-vi

Research output: Chapter in Book/Report/Conference proceedingForeword/postscriptResearch

Recombinant Expression of Trichoderma reesei Cel61A in Pichia pastoris: Optimizing Yield and N-terminal Processing

Tanghe, M., Danneels, B., Camattari, A., Glieder, A., Vandenberghe, I., Devreese, B., Stals, I. & Desmet, T., Dec 2015, In : Molecular biotechnology. 57, 11-12, p. 1010-7 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Trichoderma
Pichia
Enzymes
Mating Factor
Polysaccharides

Restriction site free cloning (RSFC) plasmid family for seamless, sequence independent cloning in Pichia pastoris

Vogl, T., Ahmad, M., Krainer, F., Schwab, H. & Glieder, A., 2015, In : Microbial cell factories . 14, p. 1-15

Research output: Contribution to journalArticleResearchpeer-review

Synthetic biology

Glieder, A., Kubicek, C. P., Mattanovich, D., Wiltschi, B. & Sauer, M., 1 Jan 2015, Springer International Publishing AG . 368 p.

Research output: Book/ReportBookResearchpeer-review

Synthetic Biology
synthetic biology
protein engineering
Protein Engineering
biocompatible materials

The unique hydroxynitrile lyase from fern

Lanfranchi, E., Winkler, M., Pavkov-Keller, T., Köhler, E-M., Diepold, M., Darnhofer, B., Steiner, K., Thallinger, G., Birner-Grünberger, R., van Pelt, S., Gruber, K. & Glieder, A., 2015.

Research output: Contribution to conferencePosterResearch

2016

Access and use of fern hydroxynitrile lyase for biocatalysis

Glieder, A., Lanfranchi, E., Winkler, M., Gruber, K. & Pavkov-Keller, T., 28 Sep 2016.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

A new family of R-selective Hydroxynitrile lyases

Lanfranchi, E., Köhler, E-M., Diepold, M., Pavkov-Keller, T., Gruber, K., Steiner, K., Glieder, A. & Winkler, M., 11 Jul 2016.

Research output: Contribution to conferencePosterResearch

A new family of R-selective Hydroxynitrile lyases

Lanfranchi, E., Eva-Maria, K., Diepold, M., Pavkov-Keller, T., Gruber, K., Steiner, K., Glieder, A. & Winkler, M., 16 Nov 2016.

Research output: Contribution to conferencePosterResearch

A Toolbox of Diverse Promoters Related to Methanol Utilization: Functionally Verified Parts for Heterologous Pathway Expression in Pichia pastoris

Vogl, T., Sturmberger, L., Kickenweiz, T., Wasmayer, R., Schmid, C., Hatzl, A-M., Gerstmann, M. A., Pitzer, J., Wagner, M., Thallinger, G. G., Geier, M. & Glieder, A., 19 Feb 2016, In : ACS Synthetic Biology . 5, 2, p. 172-86 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Pichia
Methanol
alcohol oxidase
Genes
Biosynthetic Pathways

Biotechnological advances towards an enhanced peroxidase production in Pichia pastoris

Krainer, F. W., Gerstmann, M. A., Darnhofer, B., Birner-Gruenberger, R. & Glieder, A., 10 Sep 2016, In : Journal of Biotechnology. 233, p. 181-9 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Pichia
Horseradish Peroxidase
Peroxidase
Enzymes
Methanol

Combinatorial optimization of CRISPR/Cas9 expression enables precision genome engineering in the methylotrophic yeast Pichia pastoris

Weninger, A., Hatzl, A-M., Schmid, C., Vogl, T. & Glieder, A., 10 Oct 2016, In : Journal of Biotechnology. 235, p. 139-49 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Clustered Regularly Interspaced Short Palindromic Repeats
Pichia
Guide RNA
RNA Polymerase II
Yeasts

COMPACT AND OPTIMIZED METABOLIC PATHWAY DESIGN IN PICHIA PASTORIS

Geier, M., Glieder, A. & Fauland, P., 6 May 2016, IPC No. C12N 15/ 09 A I, Patent No. WO2016066711, Priority date 28 Oct 2014, Priority No. EP20140190702

Research output: PatentResearch

Pichia
Metabolic Networks and Pathways
Yeasts
Gene Expression

Concurrent optimization strategies for high level protein production: Protein, strain and expression engineering

Weninger, A., Alkofer, A., Wimmer, R., Höppel, E., Eichmann, J. P-R., Schmid, C., Winkler, M. & Glieder, A., 22 Sep 2016.

Research output: Contribution to conferencePosterResearch

Expression of fungal cytochrome P450 variants in E. coli and their validation for API metabolite synthesis

Bachler, T., Eichmann, J. P-R., Weninger, A., Hammer, K., Tunjic, S., Glieder, A., Breinbauer, R. & Winkler, M., 12 Sep 2016.

Research output: Contribution to conferencePosterResearch

NEW HYDROXYNITRILE LYASES

Glieder, A., Lanfranchi, E., Steiner, K., Pavkov-Keller, T., Diepold, M. & Winkler, M., Feb 2016, Patent No. WO2016020528 (A1)

Research output: PatentResearch

Pichia pastoris mutants as host strains for efficient secretion of recombinant Branched Chain Aminotransferase (BCAT)

Weinhandl, K., Ballach, M., Winkler, M., Mudassar, A., Glieder, A., Birner-Gruenberger, R., Fotheringham, I. & Escalettes, F., 2016, In : Journal of Biotechnology. 235, p. 84-91 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Glycosylation
Pichia
Amino acids
Proteins
Amino Acids

Polypeptides having xylanase activity with a high conversion rate of xylose-containing polysaccharides

REISINGER, CHRISTOPH., Gamauf, C., KRAUS, MICHAEL., UNTERSTRASSER, ISABEL., MITROVIC, ALEKSANDRA. & Glieder, A., 9 Mar 2016, IPC No. C12N 9/ 24 A I, Patent No. EP2993230, Priority date 2 Sep 2014, Priority No. EP20140003017

Research output: PatentResearch

Xylose
Polysaccharides
Nucleic acid sequences
Peptides
Patents and inventions

Recombinant production of a peroxidase-protein G fusion protein in Pichia pastoris

Krainer, F., Darnhofer, B., Birner-Gruenberger, R. & Glieder, A., 10 Feb 2016, In : Journal of Biotechnology. 219, p. 24-27 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Pichia
Horseradish Peroxidase
Peroxidase
Proteins
Isoenzymes

Refined Pichia pastoris reference genome sequence

Sturmberger, L., Chappell, T., Geier, M., Krainer, F., Day, K. J., Vide, U., Trstenjak, S., Schiefer, A., Richardson, T., Soriaga, L., Darnhofer, B., Birner-Gruenberger, R., Glick, B. S., Tolstorukov, I., Cregg, J., Madden, K. & Glieder, A., 10 Oct 2016, In : Journal of Biotechnology. 235, p. 121-131 11 p.

Research output: Contribution to journalReview articleResearchpeer-review

Pichia
Genes
Genome
Chromosomes
Open Reading Frames

Scent of bitter almond and a pinch of –omics: discovering a novel hydroxynitrile lyase

Lanfranchi, E., Pavkov-Keller, T., Steiner, K., Gruber, K., Winkler, M. & Glieder, A., 11 Oct 2016.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Synergism of proteomics and mRNA sequencing for enzyme discovery

Sturmberger, L., Wallace, P. W., Glieder, A. & Birner-Gruenberger, R., 10 Oct 2016, In : Journal of Biotechnology. 235, p. 132-8 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Proteomics
Messenger RNA
Enzymes
Protein Databases
Technology

Synthetic biology for organic syntheses

Wiltschi, B. & Glieder, A., 2016, Microwave-Assisted Polymerization. 46 ed. Royal Society of Chemistry, Vol. 2016-January. p. 165-179 15 p. (RSC Green Chemistry; vol. 2016-January, no. 46).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Synthetic Biology
Synthetic Chemistry Techniques
Biosynthesis
Biological systems
Acids

Towards API Metabolite Synthesis by Fungal Cytochrome P450 variants

Bachler, T., Eichmann, J. P-R., Weninger, A., Glieder, A., Breinbauer, R. & Winkler, M., 16 Nov 2016.

Research output: Contribution to conferencePosterResearch

2017

Chemicals made by Pichia pastoris

Glieder, A., 1 Nov 2017.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris

Markošová, K., Camattari, A., Rosenberg, M., Glieder, A., Turner, N. J. & Rebroš, M., 2017, In : Biotechnology Letters. https://doi.org/10.1007/s10529-017-2450-y.

Research output: Contribution to journalArticleResearchpeer-review

Pichia
Monoamine Oxidase
Organism Cloning
Enzymes
Clone Cells

Combinatorial optimization strategies to increase Human P450 catalytic Activity in whole cell Biotransformations

Weninger, A., Alkofer, A., Schmid, C., Pranjic, M., Winkler, M. & Glieder, A., Jul 2017.

Research output: Contribution to conferencePosterResearch

Concurrent optimization strategies for high level protein production: Process, strain and expression engineering

Weninger, A., Alkofer, A., Schmid, C., Höppel, E., Winkler, M. & Glieder, A., Jun 2017.

Research output: Contribution to conferencePosterResearch

Cytochrome P450 CYP505 mutants for biooxidations of active pharmaceutical ingredients

Bachler, T., Weninger, A., Glieder, A. & Winkler, M., 21 Nov 2017.

Research output: Contribution to conferencePosterResearch

Cytochrome P450 CYP505 mutants for biooxidations of active pharmaceutical ingredients

Bachler, T., Weninger, A., Glieder, A. & Winkler, M., 5 Sep 2017.

Research output: Contribution to conferencePosterResearch

Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Lanfranchi, E., Pavkov-Keller, T., Koehler, E-M., Diepold, M., Steiner, K., Darnhofer, B., Hartler, J., Van Den Bergh, T., Joosten, H-J., Gruber-Khadjawi, M., Thallinger, G. G., Birner-Gruenberger, R., Gruber, K., Winkler, M. & Glieder, A., 10 May 2017, In : Scientific reports. 7, 46738.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
mandelonitrile lyase
Enzymes
Biocatalysis
Ferns
Crystallography

Erratum: Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Lanfranchi, E., Pavkov-Keller, T., Koehler, E-M., Diepold, M., Steiner, K., Darnhofer, B., Hartler, J., Bergh, T. V. D., Joosten, H-J., Gruber-Khadjawi, M., Thallinger, G. G., Birner-Gruenberger, R., Gruber, K., Winkler, M. & Glieder, A., 26 May 2017, In : Scientific reports. 7, 46834.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
mandelonitrile lyase
Enzymes

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Weninger, A., Fischer, J., Raschmanová, H., Kniely, C., Vogl, T. & Glieder, A., 1 Nov 2017, In : Journal of Cellular Biochemistry.

Research output: Contribution to journalArticleResearchpeer-review

Clustered Regularly Interspaced Short Palindromic Repeats
Pichia
Homologous Recombination
Guide RNA
Genes

Heme Enzymes for Biocatalysis

Glieder, A., 6 Oct 2017.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Pichia pastoris Alcohol Oxidase 1 (AOX1) Core Promoter Engineering by High Resolution Systematic Mutagenesis

Portela, R. M. C., Vogl, T., Ebner, K., Oliveira, R. & Glieder, A., 10 Nov 2017, In : Biotechnology Journal. 10.1002/biot.201700340.

Research output: Contribution to journalArticleResearchpeer-review

alcohol oxidase
Pichia
5' Untranslated Regions
Mutagenesis
Mutation

Protein expression: Yeast or E. coli

Glieder, A., 6 Oct 2017.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Substrate specificity of CYP505X from Aspergillus fumigatus

Bachler, T., Alkofer, A., Gralewska, P., Weninger, A., Glieder, A., Breinbauer, R. & Winkler, M., 11 Jul 2017.

Research output: Contribution to conferencePosterResearch