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2015

Action of an AA9 - type lytic polysaccharide monooxygenase on the cellulose surface and its effect on cellulase hydrolytic efficiency

Eibinger, M., Ganner, T., Plank, H. & Nidetzky, B., 2 Aug 2015.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

All-in-one assay for β-D-galactoside sialyltransferases: quantification of productive turnover, error hydrolysis and site selectivity

Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D. & Nidetzky, B., 2015, In : Analytical biochemistry. 483, p. 47-53

Research output: Contribution to journalArticleResearchpeer-review

Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose

Ganner, T., Sattelkow, J., Rosker, S., Eibinger, M., Chernev, B. S., Fitzek, H. M., Kraxner, J., Mayrhofer, C., Aschl, T., Nidetzky, B. & Plank, H., 2015, ASEM Workshop Advanced Electron Microscopy. ., p. 30-30

Research output: Chapter in Book/Report/Conference proceedingConference contributionResearchpeer-review

Artificial Substrates as Key Element towards Single Enzyme Tracking via High Speed Atomic Force Microscopy in Enzymatic Degradation of Cellulose

Ganner, T., Eibinger, M., Rosker, S., Sattelkow, J., Aschl, T., Rattenberger, J., Fitzek, H. M., Nidetzky, B. & Plank, H., 7 May 2015.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

C-Glycosyltransferases: From mechanistic insight to synthetic application in flavonoid glycosylation

Gutmann, A., Bungaruang, L. & Nidetzky, B., 5 Aug 2015.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Comparison of broad-scope assays of nucleotide sugar-dependent glycosyltransferases.

Czabany, T., Bubner, P., Luley, C. & Nidetzky, B., 2015, In : Analytical biochemistry. 490, p. 46-51

Research output: Contribution to journalArticleResearchpeer-review

Complete switch from alpha2,3- to alpha2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign

Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 10 May 2015.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Complete switch from alpha2,3- to alpha2,6-regioselectivity in Pasteurella dagmatis beta-D-galactoside sialyltransferase by active-site redesign

Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 4 May 2015.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 2015, In : Chemical Communications. p. 3083-3086

Research output: Contribution to journalArticleResearchpeer-review

Creating a Water-Soluble Resveratrol-Based Antioxidant by Site-Selective Enzymatic Glucosylation

Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 2015, In : ChemBioChem. 16, 13, p. 1870-1874

Research output: Contribution to journalArticleResearchpeer-review

Antioxidants
Water
Solubility
Glycosylation
Glucosyltransferases

Critical evaluation of different NDP-sugar purification strategies

Lemmerer, M., Schmoelzer, K., Gutmann, A., Lepak, A. & Nidetzky, B., 2015.

Research output: Contribution to conferencePosterResearch

Critical evaluation of different NDP-sugar purification strategies

Lemmerer, M., Schmoelzer, K., Gutmann, A., Lepak, A. & Nidetzky, B., 2015.

Research output: Contribution to conferencePosterResearch

Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

Wildberger, P., Pfeiffer, M., Brecker, L. & Nidetzky, B., 21 Dec 2015, In : Angewandte Chemie / International Edition . 54, 52, p. 15867-15871 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Phosphorylase Phosphatase
Phosphatases
Phosphates
Phosphorylation
Catalysts

Expression of human sialyltransferase in yeast

Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D., Schwab, H. & Nidetzky, B., 21 May 2015.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

FUCOSYLTRANSFERASES: KEY PLAYERS IN THE BIOECHNOLOGICAL SYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES

Petschacher, B., Kuballa, J. & Nidetzky, B., 2015.

Research output: Contribution to conferencePosterResearch

Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts

Trausinger, G., Gruber, C., Krahulec, S., Magnes, C., Nidetzky, B. & Klimacek, M., 2015, In : Biotechnology for Biofuels . 8:157, p. 1-13

Research output: Contribution to journalArticleResearchpeer-review

Mesoporous Silica Materials Labeled for Optical Oxygen Sensing and Their Application to Development of a Silica-Supported Oxidoreductase Biocatalyst

Bolivar, J. M., Schelch, S., Mayr, T. & Nidetzky, B., 2 Oct 2015, In : ACS Catalysis. 5, 10, p. 5984-5993 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Biocatalysts
Silicon Dioxide
Oxidoreductases
Silica
Oxygen

N-​terminally truncated glycosyltransferases for use in post-​translational sialylation

Czabany, T., Engel, A., Greif, M., Jung, C., Luley, C., Malik, S., Mueller, R., Nidetzky, B., Ribitsch, D., Schmoelzer, K., Schwab, H., Sobek, H., Suppmann, B., Thomann, M. & Zitzenbacher, S., 8 Jan 2015, Patent No. WO 2015001034 A1

Research output: PatentResearch

Oxidation of monolignols by members of the berberine bridge enzyme family suggests a role in plant cell wall metabolism

Daniel, B., Pavkov-Keller, T., Steiner, B., Dodic, A., Gutmann, A., Nidetzky, B., Sensen, C. W., van der Graaff, E., Wallner, S., Gruber, K. & Macheroux, P., 2015, In : The Journal of Biological Chemistry. 290, 30, p. 18770-18781

Research output: Contribution to journalArticleResearchpeer-review

Phosphoryl transfer from α-D-glucose 1-phosphate catalyzed by Escherichia coli sugar-phosphate phosphatases of two protein superfamily types

Wildberger, P., Pfeiffer, M., Brecker, L., Rechberger, G. N., Birner-Gruenberger, R. & Nidetzky, B., 2015, In : Applied and Environmental Microbiology. 81, 5, p. 1559-1572 14 p.

Research output: Contribution to journalArticleResearchpeer-review

glucose 1-phosphate
Sugar Phosphates
sugar phosphates
phosphatase
sugar

Probing of the reaction pathway of human UDP-xylose synthase with site-directed mutagenesis

Eixelsberger, T., Weber, H. & Nidetzky, B., 2015, In : Carbohydrate Research. 416, p. 1-6

Research output: Contribution to journalArticleResearchpeer-review

Process for the mono- and bi-​sialylation of glycoproteins employing N-​terminally truncated β-​galactoside α-​2,​6-​sialyltransferase mutants

Czabany, T., Engel, A., Greif, M., Jung, C., Luley, C., Malik, S., Mueller, R., Nidetzky, B., Ribitsch, D., Schmoelzer, K., Schwab, H., Sobek, H., Suppmann, B., Thomann, M. & Zitzenbacher, S., 8 Jan 2015, Patent No. WO 2015001033 A1

Research output: PatentResearch

Rules for biocatalyst and reaction engineering to implement effective, NAD(P)H-dependent, whole cell bioreductions

Kratzer, R., Woodley, J. & Nidetzky, B., 2015, In : Biotechnology Advances. 33, 8, p. 1641–1652

Research output: Contribution to journalArticleResearchpeer-review

Saturation Transfer Difference NMR to Study Substrate and Product Binding to Human UDP-Xylose Synthase (hUXS1A) During Catalytic Event

Puchner, C., Eixelsberger, T., Nidetzky, B. & Brecker, L., 2015, In : RSC Advances . 5, p. 86919-86926

Research output: Contribution to journalArticleResearchpeer-review

Structural and biochemical characterization of a monolignol oxidoreductase from A. thaliana implies a link between the berberine bridge enzyme-like family and cell wall metabolism

Daniel, B., Pavkov-Keller, T., Steiner, B., Dordic, A., Gutmann, A., Nidetzky, B., Sensen, C. W., van der Graaff, E., Wallner, S., Gruber, K. & Macheroux, P., 2015.

Research output: Contribution to conferencePosterResearch

Sucrose synthase: a unique glycosyltransferase enroute to biocatalytic process development

Schmoelzer, K., Gutmann, A., Lepak, A., Lemmerer, M. & Nidetzky, B., 2015.

Research output: Contribution to conferencePosterResearch

Systematic strain construction and process development: Xylitol production by Saccharomyces cerevisiae expressing Candida tenuis xylose reductase in wild-type or mutant form

Pratter, S., Eixelsberger, T. & Nidetzky, B., 2015, In : Bioresource Technology. 198, p. 732-738

Research output: Contribution to journalArticleResearchpeer-review

The berberine bridge enzyme-like superfamily: A multi gene family involved in lignification, drought and pathogen response

Daniel, B., Steiner, B., Pavkov-Keller, T., Dordic, A., Gutmann, A., Nidetzky, B., Sensen, C. W., van der Graaff, E., Wallner, S., Gruber, K. & Macheroux, P., 2015.

Research output: Contribution to conferencePosterResearch

The Visualization of Enzymatic Cellulose Degradation Using High Speed Atomic Force Microscopy

Ganner, T., Sattelkow, J., Aschl, T., Kraxner, J., Eibinger, M., Mayrhofer, C., Chernev, B. S., Bubner, P., Nidetzky, B. & Plank, H., 2015.

Research output: Contribution to conferencePosterResearch

Towards efficient flavonoid glycosylation through in situ sugar donor recycling

Gutmann, A., Bungaruang, L. & Nidetzky, B., 2015.

Research output: Contribution to conferencePosterResearch

Tunable Semicrystalline Thin Film Cellulose Substrate for High- Resolution, In-Situ AFM Characterization of Enzymatic Cellulose Degradation

Ganner, T., Rosker, S., Eibinger, M., Kraxner, J., Sattelkow, J., Rattenberger, J., Fitzek, H. M., Chernev, B. S., Grogger, W., Nidetzky, B. & Plank, H., 2015, In : ACS Applied Materials & Interfaces. 7, 50, p. 27900-27909

Research output: Contribution to journalArticleResearchpeer-review

2016

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

Bolivar, J. M., Eisl, I. & Nidetzky, B., 1 Jan 2016, In : Catalysis Today. 259, p. 66-80 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Immobilized Enzymes
Biocatalysts
Enzymes
Catalyst supports
Proteins
Fucosyltransferases
Oligosaccharides
Human Milk
galactoside 3-fucosyltransferase
Guanosine Diphosphate Fucose

Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution

Eibinger, M., Zahel, T., Ganner, T., Plank, H. & Nidetzky, B., 8 Mar 2016, In : Biotechnology for Biofuels . 9, 56

Research output: Contribution to journalArticleResearchpeer-review

CREATING A WATER-SOLUBLE RESVERATROL-BASED ANTIOXIDANT THROUGH SITE-SELECTIVE ENZYMATIC GLUCOSYLATION

Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 14 Jul 2016.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Development of a fully integrated falling film microreactor for gas-liquid-solid biotransformation with surface immobilized O2-dependent enzyme

Bolivar Bolivar, J. M., Krämer, C. E. M., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering.

Research output: Contribution to journalArticleResearchpeer-review

Biotransformation
Enzymes
Gases
Immobilization
Oxidoreductases

Direct-Write Fabrication of Cellulose Nano-Structures via Focused Electron Beam Induced Nanosynthesis

Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2 Sep 2016, In : Scientific reports. p. 1-11 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Downstream Processing of Nucleoside-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption

Lemmerer, M., Schmoelzer, K., Gutmann, A. & Nidetzky, B., 2016, In : Advanced Synthesis & Catalysis. 358, p. 3113-3122

Research output: Contribution to journalArticleResearchpeer-review

Sugar (sucrose)
Nucleosides
Sugars
Chromatography
Anions

Exploiting the biocatalytic potential of glycosyltransferases through in situ sugar donor recycling

Gutmann, A., Bungaruang, L., Lepak, A. & Nidetzky, B., 14 Nov 2016.

Research output: Contribution to conferencePosterResearch

Glycosyltransferases
Diphosphates
Uridine
Sugars
Recycling

Fabrication of Cellulose Structures via Focused Electron Beam Induced Conversion: Approaching the Nanoscale

Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2016.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Functional characterization of thenative swollenin from Trichoderma reesei:study of its possible role as C1 factor ofenzymatic lignocellulose conversion

Eibinger, M., Sigl, K., Sattelkow, J., Ganner, T., Ramoni, J., Seiboth, B., Plank, H. & Nidetzky, B., 2016, In : Biotechnology for Biofuels . p. 9:178

Research output: Contribution to journalArticleResearchpeer-review

Improving the Water Solubility of Polyphenols through Site-Selective Enzymatic Glucosylation

Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 12 Jun 2016.

Research output: Contribution to conferencePosterResearch

Kinetic analysis and probing with substrate analogues of the reaction pathway of the nitrile reductase QueF from Escherichia coli

Jung, J., Czabany, T., Wilding, B., Klempier, N. & Nidetzky, B., 2016, In : The Journal of Biological Chemistry. 291, 49, p. 25411-25426 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Nitriles
Escherichia coli
Oxidoreductases
Kinetics
Substrates

Let the substrate flow, not the enzyme: Practical immobilization of d-amino acid oxidase in a glass microreactor for effective biocatalytic conversions

Bolivar Bolivar, J. M., Tribulato, M. A., Petrasek, Z. & Nidetzky, B., 1 Nov 2016, In : Biotechnology and Bioengineering. 113, 11, p. 2342-2349 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Enzyme immobilization
Immobilization
Glass
Amino acids
Oxidoreductases

Prokaryotic fucosyltransferases for biotechnological production of fucosylated human milk oligosaccharides

Petschacher, B., Gierlinger, K., Brunner, L., Rinner, G., Kuballa, J. & Nidetzky, B., 13 Sep 2016.

Research output: Contribution to conferencePosterResearchpeer-review

Prokaryotic fucosyltransferases for biotechnological production of fucosylated human milk oligosaccharides

Petschacher, B., Gierlinger, K., Brunner, L., Rinner, G., Kuballa, J. & Nidetzky, B., 14 Nov 2016.

Research output: Contribution to conferencePosterResearch

Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1

Dewitte, G., Walmagh, M., Diricks, M., Lepak, A., Gutmann, A., Nidetzky, B. & Desmet, T., 1 Jun 2016, In : Journal of Biotechnology. 233, p. 49-55

Research output: Contribution to journalArticleResearchpeer-review

Stevia
Glycosylation
Glycosyltransferases
Uridine Diphosphate
Glycosides

Solubility Enhancement by Site-Selective Polyphenol Glucosylation

Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 11 Feb 2016.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch