FWF - Funktionsselektive - Funktion-Selective Protein Scavenger Nanolayers

Project: Research project

Description

Aim of the work is to provide reliable and cheap glycosidase detecting systems for high-throughput as well as
micro-array/chip applications for a wide range of diagnostic purposes in biochemistry, medicine and "functional
proteomics". Selected REVERSIBLE glycosidase inhibitors carrying a fluorescent tag will be chemically
synthesised, biologically evaluted, screened for applicability in various simple anylytical test systems and
ultimately connected to a chip surface. Exposure to pure enzymes, mixtures or "general" biological matrices result
in binding events which can directly be measured by fluorescence spectrometry analysis. From this, a "glycosidase
atlas" will later be available to identify glycosidases by their unique, finger-print-like patterns of interaction with
the chip-bound inhibitors. After clear "proof of concept", the method can be applied to special diagnostic purposes
and problems. For example, "viral enzyme chips" (influenza, herpes, HIV), "hereditary metabolic disease chips"
(brain metabolism, post translational glycoprotein processing) or "cancer-associated enzyme chips" can be
envisaged to be based on this approach. Relying on the application of reversible inhibitors for detection, snap-shots
for time-resolved studies/diagnostics will also be available. Furthermore, on the same basis, the chip may be used
several times (as opposed to "one-way" devices), allowing for additional diagnostic options as well as "costcutting"
in "low-budget" situations (poor income systems such as NGOs or under-developed countries and
societies).
StatusFinished
Effective start/end date1/04/061/04/09