Continuous Ex Vivo Lactate Monitoring in the Interstitial Fluid

      Project: Research project

      Description

      Monitoring of adipose tissue lactate concentration during exercise was not possible so far. The aim of this study was to evaluate the measurement of subcutaneous lactate concentration during exercise by combining open flow microperfusion, lactate and conductivity sensoring. The lactate sensor was based on the measurement of H2O2 which is produced by the membrane-enclosed enzyme lactate oxidase. The conductivity sensor was used to calculate the recovery of the sampled interstitial fluid. In 5 healthy subjects, the microperfusion catheter was inserted into the adipose tissue and the electronic unit was fixed at the hips. The subjects started with a 6 min warm up at 50 watts. The exercise was then increased by 50 watts every 3 min until exhaustion. The results show that during exercise the lactate concentration in the interstitial fluid can be measured on line and continuously by this method.
      StatusFinished
      Effective start/end date1/01/9631/12/99