Robert Kourist

Univ.-Prof. Dr.rer.nat.

20052022
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Research Output 2005 2019

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2019

Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol

Horvat, M., Fritsche, S., Kourist, R. & Winkler, M., 19 Mar 2019, In : ChemCatChem. p. 4171-4181

Research output: Contribution to journalArticleResearchpeer-review

carboxylates
Oxidoreductases
preparation
cells
aldehydes

Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator

Assil Companioni, L., Schmidt, S., Heidinger, P., Schwab, H. & Kourist, R., 19 Mar 2019, In : ChemSusChem. 12, 11, p. 2361-2365 5 p.

Research output: Contribution to journalArticleResearchpeer-review

biotransformation
Hydrogen
regeneration
hydrogen
Hydrogenase
2018

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

Research output: Contribution to journalArticleResearchpeer-review

decarboxylation
Carboxy-Lyases
fatty acids
Hydrocarbons
Fatty acids

Cloning and characterization of a new delta-specific L-leucine dioxygenase from Anabaena variabilis

Correia Cordeiro, R. S., Enoki, J., Busch, F., Mügge, C. & Kourist, R., 20 Oct 2018, In : Journal of Biotechnology. 284, p. 68-74 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Anabaena variabilis
Dioxygenases
Cloning
Leucine
Amino acids

Raman Microspectroscopic Evidence for the Metabolism of a Tyrosine Kinase Inhibitor, Neratinib, in Cancer Cells

Aljakouch, K., Lechtonen, T., Yosef, H. K., Hammoud, M. K., Alsaidi, W., Kötting, C., Mügge, C., Kourist, R., El-Mashtoly, S. F. & Gerwert, K., 11 Jun 2018, In : Angewandte Chemie / International Edition . 57, 24, p. 7250-7254 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Metabolites
Metabolism
Protein-Tyrosine Kinases
Microscopic examination
Cells
2017

Durch Trennen verbinden

Schmidt, S., Gröger, H. & Kourist, R., Oct 2017, In : Nachrichten aus der Chemie. 65, 10, p. 985-988 4 p.

Research output: Contribution to journalArticleResearch

Enzymatic Oxyfunctionalization Driven by Photosynthetic Water-Splitting in the Cyanobacterium Synechocystis sp. PCC 6803

Böhmer, S., Köninger, K., Gomez-Baraibar, A., Bojarra, S., Mügge, C., Schmidt, S., Nowaczyk, M. & Kourist, R., Aug 2017, In : Catalysts.

Research output: Contribution to journalArticleResearchpeer-review

Overcoming the incompatibility challenge in chemo-enzymatic and multi-catalytic cascade reactions.

Schmidt, S., Castiglione, K. & Kourist, R., 6 Sep 2017, In : Chemistry - A European Journal.

Research output: Contribution to journalArticleResearchpeer-review

Transcriptome Profiling of the Australian arid-land Plant Eremophila serrulata for the Identification of Monoterpene synthases

Kracht, O. N., Ammann, A-C., Stockmann, J., Wibberg, D., Kalinowski, J., Piotrowski, M., Kerr, R., Brück, T. B. & Kourist, R., 2017, In : Phytochemistry. 136, p. 15-22

Research output: Contribution to journalArticleResearchpeer-review

2016

A Multi-Enzymatic Cascade Reaction for the Stereoselective Production of γ-Oxyfunctionalyzed Amino Acids

Enoki, J., Meisborn, J., Müller, A-C. & Kourist, R., 2016, In : Frontiers in Microbiology . 7, p. 425

Research output: Contribution to journalArticleResearchpeer-review

Amino Acids
Geobacillus
Enzymes
Racemases and Epimerases
Dioxygenases

A One-Pot Cascade Reaction Combining an Encapsulated Decarboxylase with a Metathesis Catalyst for the Synthesis of Bio-Based Antioxidants

Gómez Baraibar, Á., Reichert, D., Mügge, C., Seger, S., Gröger, H. & Kourist, R., 14 Nov 2016, In : Angewandte Chemie / International Edition . 55, 47, p. 14823-14827 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Carboxy-Lyases
Antioxidants
Organic solvents
Catalysts
Ruthenium

Arylmalonate decarboxylase-a highly selective bacterial biocatalyst with unknown function

Miyamoto, K. & Kourist, R., Oct 2016, In : Applied Microbiology and Biotechnology. 100, 20, p. 8621-31 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Enzymes
Carboxy-Lyases
Carboxylic Acids
malonate decarboxylase

Arylmalonate decarboxylase-catalyzed asymmetric synthesis of both enantiomers of optically pure flurbiprofen

Gaßmeyer, S. K., Wetzig, J., Mügge, C., Assmann, M., Enoki, J., Hilterhaus, L., Zuhse, R., Miyamoto, K., Liese, A. & Kourist, R., 2016, In : ChemCatChem. 8, p. 916-921 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Identification of amino acid networks governing catalysis in the closed complex of class I terpene synthases

Schrepfer, P., Buettner, A., Goerner, C., Hertel, M., van Rijn, J., Wallrapp, F., Eisenreich, W., Sieber, V., Kourist, R. & Brück, T., 23 Feb 2016, In : Proceedings of the National Academy of Sciences of the United States of America. 113, 8, p. E958-67

Research output: Contribution to journalArticleResearchpeer-review

Catalysis
Terpenes
Amino Acids
Computer Simulation
Catalytic Domain

Lichtgetriebene Ganzzellbiotransformation mit rekombinanten Cyanobakterien

Nowaczyk, M. & Kourist, R., 2016, In : Biospektrum. 22, 7, p. 765-767 3 p.

Research output: Contribution to journalArticleResearch

Light-driven Enzymatic Decarboxylation

Köninger, K., Grote, M., Zachos, I., Hollmann, F. & Kourist, R., 22 May 2016, In : Journal of visualized experiments . 111

Research output: Contribution to journalArticleResearchpeer-review

Decarboxylation
Hydrogen Peroxide
Fatty Acids
Fatty acids
Enzymes

Recombinant Cyanobacteria for the Asymmetric Reduction of C=C Bonds Fueled by the Biocatalytic Oxidation of Water

Köninger, K., Gómez Baraibar, Á., Mügge, C., Paul, C. E., Hollmann, F., Nowaczyk, M. M. & Kourist, R., 25 Apr 2016, In : Angewandte Chemie / International Edition . 55, 18, p. 5582-5 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Oxidoreductases
Oxidation
Photosynthesis
Water
NADP

Semiempirical QM/MM calculations reveal a step-wise proton transfer and an unusual thiolate pocket in the mechanism of the racemizing mutant G74C of arylmalonate decarboxylase

Busch, F., Hülsemann, N., Enoki, J., Miyamoto, K., Bocola, M. & Kourist, R., 2016, In : Catalysis Science & Technology. 6, p. 4937-4944 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Sequence-Based Screening for Rare Enzymes: New Insights into the World of AMDases Reveal a Conserved Motif and 58 Novel Enzymes Clustering in Eight Distinct Families

Maimanakos, J., Chow, J., Gaßmeyer, S. K., Güllert, S., Busch, F., Kourist, R. & Streit, W. R., 2016, In : Frontiers in Microbiology . 7, p. 1332

Research output: Contribution to journalArticleResearchpeer-review

Cluster Analysis
Enzymes
aspartate racemase
Genes
glutamate racemase
2015

A combined bioinformatics and functional metagenomics approach to discovering lipolytic biocatalysts

Masuch, T., Kusnezowa, A., Nilewski, S., Bautista, J. T., Kourist, R. & Leichert, L. I., 2015, In : Frontiers in Microbiology . 6

Research output: Contribution to journalArticleResearchpeer-review

Draft Genome Sequence of Bordetella bronchiseptica KU1201, the First Isolation Source of Arylmalonate Decarboxylase

Yoshida, S., Enoki, J., Hemmi, R., Kourist, R., Kawakami, N. & Miyamoto, K., 7 May 2015, In : Genome Announcements. 3, 3

Research output: Contribution to journalArticleResearchpeer-review

Bordetella bronchiseptica
Genome
Bacteria
Proteins
malonate decarboxylase

Engineered hydrophobic pocket of (S)-selective arylmalonate decarboxylase variant by simultaneous saturation mutagenesis to improve catalytic performance

Yoshida, S., Enoki, J., Kourist, R. & Miyamoto, K., 2015, In : Bioscience, Biotechnology and Biochemistry. 79, 12, p. 1965-1971 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Expression of enantioselective enzymes in cyanobacteria

Bartsch, M., Gaßmeyer, S. K., Igarashi, K., Miyamoto, M., Rögner, M., Nowaczyc, M. & Kourist, R., 2015, In : Microbial cell factories . 14, p. 53 1 p.

Research output: Contribution to journalArticleResearchpeer-review

Genomics and Transcriptomics Analyses of the Oil-Accumulating Basidiomycete Yeast Trichosporon oleaginosus: Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems: Insights into Substrate Utilization and Alternative Evolutionary Trajectories of Fungal Mating Systems

Kourist, R., Bracharz, F., Lorenzen, J., Kracht, O. N., Chovatia, M., Daum, C., Deshpande, S., Lipzen, A., Nolan, M., Ohm, R. A., Grigoriev, I. V., Sun, S., Heitman, J., Brück, T. & Nowrousian, M., 21 Jul 2015, In : mBio. 6, 4, p. e00918

Research output: Contribution to journalArticleResearchpeer-review

Trichosporon
Basidiomycota
Genomics
Oils
Yeasts

Photobiocatalytic decarboxylation for olefin synthesis

Zachos, I., Gaßmeyer, S. K., Bauer, D., Sieber, V., Hollmann, F. & Kourist, R., 2015, In : Chemical communications. 51, p. 1918-1921 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Photosynthetic production of enantioselective biocatalysts

Bartsch, M., Gassmeyer, S. K., Köninger, K., Igarashi, K., Liauw, P., Dyczmons-Nowaczyk, N., Miyamoto, K., Nowaczyk, M. M. & Kourist, R., 15 Apr 2015, In : Microbial cell factories . 14, p. 53

Research output: Contribution to journalArticleResearchpeer-review

Biocatalysts
Enzymes
Synechocystis
Biofuels
Cyanobacteria

STD-NMR-Based Protein Engineering of the Unique Arylpropionate-Racemase AMDase G74C

Gaßmeyer, S. K., Yoshikawa, H., Enoki, J., Hülsemann, N., Stoll, R., Miyamoto, K. & Kourist, R., 23 Jun 2015, In : ChemBioChem. 16, 3, p. 1943-1949

Research output: Contribution to journalArticleResearchpeer-review

Racemases and Epimerases
Protein Engineering
Nuclear magnetic resonance
Amino Acids
Substrates
2014

Enzymatic decarboxylation—an emerging reaction for chemicals production from renewable resources

Kourist, R., Guterl, J-K., Miyamoto, K. & Sieber, V., 2014, In : ChemCatChem. 6, 3, p. 689-701 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipases as Sustainable Biocatalysts for the Sustainable Industrial Production of Fine Chemicals and Cosmetics

Kourist, R., Hollmann, F. & Nguyen, G. S., 2014, In : JSM Biotechnology & Biomedical Engineering. 2, p. 1029 1 p.

Research output: Contribution to journalArticleResearchpeer-review

Production of macrocyclic sesqui‐and diterpenes in heterologous microbial hosts: A systems approach to harness Nature’s molecular diversity

Brück, T., Kourist, R. & Loll, B., 2014, In : ChemCatChem. 6, 5, p. 1142-1165 24 p.

Research output: Contribution to journalArticleResearchpeer-review

2013

Thermally driven asymmetric domino reaction catalyzed by a thermostable esterase and its variants

Wada, R., Kumon, T., Kourist, R., Ohta, H., Uemura, D., Yoshida, S. & Miyamoto, K., 2013, In : Tetrahedron letters. 54, 15, p. 1921-1923 3 p.

Research output: Contribution to journalArticleResearchpeer-review

2012

Creation of a lipase highly selective for trans fatty acids by protein engineering

Brundiek, H. B., Evitt, A. S., Kourist, R. & Bornscheuer, U. T., 9 Jan 2012, In : Angewandte Chemie / International Edition . 51, 2, p. 412-4 3 p.

Research output: Contribution to journalArticleResearchpeer-review

Saturated fatty acids
Trans Fatty Acids
Vegetable oils
Lipases
Lipase

Pseudomonas putida esterase contains a GGG(A)X-motif confering activity for the kinetic resolution of tertiary alcohols

Rehdorf, J., Behrens, G. A., Nguyen, G-S., Kourist, R. & Bornscheuer, U. T., Feb 2012, In : Applied Microbiology and Biotechnology. 93, 3, p. 1119-26 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Pseudomonas putida
Esterases
Acetates
Carboxylic Ester Hydrolases
Alcohols

The short form of the recombinant CAL-A-type lipase UM03410 from the smut fungus Ustilago maydis exhibits an inherent trans-fatty acid selectivity

Brundiek, H., Saß, S., Evitt, A., Kourist, R. & Bornscheuer, U. T., Apr 2012, In : Applied Microbiology and Biotechnology. 94, 1, p. 141-50 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Ustilago
Trans Fatty Acids
Lipase
Candida
Fungi
2011

Biocatalytic strategies for the asymmetric synthesis of profens – recent trends and developments

Kourist, R., Domínguez de María, P. & Miyamoto, K., 2011, In : Green chemistry. p. 2607-2618 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Biocatalytic synthesis of optically active tertiary alcohols

Kourist, R. & Bornscheuer, U. T., 2011, In : Applied Microbiology and Biotechnology. 91, 3, p. 505-17 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Alcohols
Biocatalysis
Acetylesterase
Hydro-Lyases
Thiamine Pyrophosphate

Comparative analysis of tertiary alcohol esterase activity in bacterial strains isolated from enrichment cultures and from screening strain libraries.

Herter, S., Nguyen, G-S., Thompson, M. L., Steffen-Munsberg, F., Schauer, F., Bornscheuer, U. T. & Kourist, R., 2011, In : Applied Microbiology and Biotechnology. 90, p. 929-939 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Esterases
Libraries
Alcohols
Esters
Hydrolysis

Dramatically improved catalytic activity of an artificial (S)-selective arylmalonate decarboxylase by structure-guided directed evolution.

Miyauchi, Y., Kourist, R., Uemura, D. & Miyamoto, K., 2011, In : Chemical communications. 47, p. 7503-7505 3 p.

Research output: Contribution to journalArticleResearchpeer-review

Naproxen
malonate decarboxylase

Engineering the promiscuous racemase activity of an arylmalonate decarboxylase

Kourist, R., Miyauchi, Y., Uemura, D. & Miyamoto, K., 10 Jan 2011, In : Chemistry - A European Journal. 17, 2, p. 557-63 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Racemases and Epimerases
Substrates
Carboxylic Acids
Carboxylic acids
Bearings (structural)

Identification of novel esterases for the synthesis of sterically demanding chiral alcohols by sequence-structure guided genome mining

Nguyen, G-S., Thompson, M. L., Grogan, G., Bornscheuer, U. T. & Kourist, R., 2011, In : Journal of molecular catalysis / B. 70, p. 88-94 7 p.

Research output: Contribution to journalArticleResearchpeer-review

One-step enzyme extraction and immobilization for biocatalysis applications.

Cassimjee, K. E., Kourist, R., Lindberg, D., Wittrup Larsen, M., Thanh, N. H., Widersten, M., Bornscheuer, U. T. & Berglund, P., 2011, In : Molecular biotechnology. 6, p. 463-469 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Biocatalysis
Immobilization
Lipases
Enzymes
Lipase

Protein engineering of arylmalonate decarboxylase G74C, an artificial racemase

Kourist, R. & Miyamoto, K. ., 2011, In : Biotechnology International. 23, p. 6-9 4 p.

Research output: Contribution to journalArticleResearchpeer-review

2010

An Enzymatic Toolbox for the Kinetic Resolution of 2-(Pyridin- x -yl)but-3-yn-2-ols and Tertiary Cyanohydrins

Nguyen, G-S., Kourist, R., Paravidino, M., Hummel, A., Rehdorf, J., Orru, R. V. A., Hanefeld, U. & Bornscheuer, U. T., 2010, In : European journal of organic chemistry. p. 2753-2758 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Enantioselective kinetic resolution of phenylalkyl carboxylic acids using metagenome-derived esterases.

Fernández-Álvaro, E., Kourist, R., Winter, J., Böttcher, D., Liebeton, K., Naumer, C., Eck, J., Leggewie, C., Jaeger, K-E., Streit, W. & Bornscheuer, U. T., 2010, In : Microbial Biotechnology. 3, p. 59-64 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Metagenome
Esterases
Carboxylic Acids
Carboxylic acids
Kinetics

Formation of chiral tertiary homoallylic alcohols via Evans aldol reaction or enzymatic resolution and their influence on the Sharpless asymmetric dihydroxylation

Theurer, M., Fischer, P., Baro, A., Nguyen, G. S., Kourist, R., Bornscheuer, U. & Laschat, S., 2010, In : Tetrahedron. 66, p. 3814-3823 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Protein engineering and discovery of lipases

Kourist, R., Brundiek, H. & Bornscheuer, U. T., 2010, In : European journal of lipid science and technology. 112, p. 64-74 11 p.

Research output: Contribution to journalArticleResearchpeer-review

The alpha/beta-hydrolase fold 3DM database (ABHDB) as a tool for protein engineering

Kourist, R., Jochens, H., Bartsch, S., Kuipers, R., Padhi, S. K., Gall, M., Böttcher, D., Joosten, H. J. & Bornscheuer, U. T., 2010, In : ChemBioChem. 11, p. 1635-1643 9 p.

Research output: Contribution to journalArticleResearchpeer-review

The role of the GGGX motif in determining the activity and enantioselectivity of pig liver esterase towards tertiary alcohols

Gall, M., Kourist, R., Schmidt, M. & Bornscheuer, U. T., 2010, In : Biocatalysis and Biotransformation. 28, p. 201-208 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Enantioselectivity
Esterases
Liver
Alcohols
Swine
2009

Directed evolution and rational design

Kourist, R., Höhne, M. & Bornscheuer, U. T., 2009, In : Chemie in unserer Zeit. 43, p. 132-142 11 p.

Research output: Contribution to journalArticleResearchpeer-review