Margit Winkler

Priv.-Doz. Dipl.-Ing. Dr.techn.

19992019
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Research Output 2001 2019

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2019

A functionally-distinct carboxylic acid reductase (PcCAR4) unearthed from a repertoire of type IV CARs in the white-rot fungus Pycnoporus cinnabarinus

Ling, J. G., Mansor, M. H., Murad, M. A., Khalid, R. M., Quay, D. H-X., Winkler, M. & Bakar, F. D. A., 2019, In : Journal of Biotechnology. 2020, 307, p. 55-62

Research output: Contribution to journalArticleResearchpeer-review

Pycnoporus
Fungi
Carboxylic acids
Genes
Enzymes

Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol

Horvat, M., Fritsche, S., Kourist, R. & Winkler, M., 19 Mar 2019, In : ChemCatChem. p. 4171-4181

Research output: Contribution to journalArticleResearchpeer-review

carboxylates
Oxidoreductases
preparation
cells
aldehydes

Co-factor demand and regeneration in the enzymatic one-step reduction of carboxylates to aldehydes in cell-free systems

Strohmeier, G., Schwarz, A., Andexer, J. N. & Winkler, M., 28 Oct 2019, (Accepted/In press) In : Journal of Biotechnology. 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Cell-Free System
Aldehydes
Regeneration
Adenosine Triphosphate
Sinorhizobium meliloti

Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Rinnofner, C., Kerschbaumer, B., Weber, H., Glieder, A. & Winkler, M., 2019, In : Biocatalysis and Agricultural Biotechnology. 17, p. 525-528 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
ibuprofen
Hydroxylation
Pichia pastoris
Biocatalysts
Pichia

Discovery of carboxylic acid reductase (CAR) from Thermothelomyces thermophilus and its evaluation for vanillin synthesis

Horvat, M., Fiume, G., Fritsche, S. & Winkler, M., 12 Aug 2019, In : Journal of Biotechnology. 304, p. 44-51

Research output: Contribution to journalArticleResearchpeer-review

Carboxylic acids
Substrates
Vanillic Acid
Eugenol
Acids

Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH

Strohmeier, G., Eiteljörg, I., Schwarz, A. & Winkler, M., 26 Apr 2019, In : Chemistry - A European Journal. 25, 24, p. 6119-6123 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Adenosinetriphosphate
Aldehydes
NADP
Carboxylic Acids
Adenosine Triphosphate

Exploring the biocatalytic potential of a cytochrome P450 from Thermothelomyces thermophila

Fürst, M. J. L. J., Kerschbaumer, B., Rinnofner, C., Migglautsch, A. K., Winkler, M. & Fraaije, M. W., 2019, (Accepted/In press) In : Advanced Synthesis & Catalysis. p. 2487 2496 p.

Research output: Contribution to journalArticleResearchpeer-review

Random Mutagenesis-Driven Improvement of Carboxylate Reductase Activity Using an Amino Benzamidoxime-Mediated High-Throughput Assay

Schwendenwein, D., Ressmann, A. K., Dörr, M., Höhne, M., Mihovilovic, M. D., Bornscheuer, U. T., Rudroff, F. & Winkler, M., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2544-2549 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Mutagenesis
Carboxylic acids
Assays
Oxidoreductases
Throughput

Substrate-Independent High-Throughput Assay for the Quantification of Aldehydes

Ressmann, A. K., Schwendenwein, D., Leonhartsberger, S., Mihovilovic, M. D., Bornscheuer, U. T., Winkler, M. & Rudroff, F., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2538-2543 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Aldehydes
Carboxylic acids
Assays
Throughput
Substrates
2018

Aliphatic hydroxylation and epoxidation of capsaicin by Cytochrome P450 CYP505X

Migglautsch, A. K., Willim, M., Schweda, B., Glieder, A., Breinbauer, R. & Winkler, M., 31 Aug 2018, In : Tetrahedron. 74, p. 6199-6204

Research output: Contribution to journalArticleResearchpeer-review

Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

Liu, Y., Gong, R., Liu, X., Zhang, P., Zhang, Q., Cai, Y-S., Deng, Z., Winkler, M., Wu, J. & Chen, W., 2018, In : Microbial cell factories . 17, 10 p., 131.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Identification of key residues for enzymatic carboxylate reduction

Stolterfoht, H., Steinkellner, G., Schwendenwein, D., Pavkov-Keller, T., Gruber, K. & Winkler, M., 12 Feb 2018, In : Frontiers in Microbiology . 9, 250.

Research output: Contribution to journalArticleResearchpeer-review

Production of Hydroxynitrile Lyase from Davallia tyermannii (DtHNL) in Komagataella phaffii and Its Immobilization as a CLEA to Generate a Robust Biocatalyst

Lanfranchi, E., Grill, B., Raghoebar, Z., Van Pelt, S., Sheldon, R. A., Steiner, K., Glieder, A. & Winkler, M., 2018, In : ChemBioChem. 19, 4, p. 312-316 10.1002/cbic.201700419.

Research output: Contribution to journalArticleResearchpeer-review

mandelonitrile lyase
Immobilization
Enzymes
Ferns
Agrochemicals
2017

Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Lanfranchi, E., Pavkov-Keller, T., Koehler, E-M., Diepold, M., Steiner, K., Darnhofer, B., Hartler, J., Van Den Bergh, T., Joosten, H-J., Gruber-Khadjawi, M., Thallinger, G. G., Birner-Gruenberger, R., Gruber, K., Winkler, M. & Glieder, A., 10 May 2017, In : Scientific reports. 7, 46738.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
mandelonitrile lyase
Enzymes
Biocatalysis
Ferns
Crystallography

Erratum: Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Lanfranchi, E., Pavkov-Keller, T., Koehler, E-M., Diepold, M., Steiner, K., Darnhofer, B., Hartler, J., Bergh, T. V. D., Joosten, H-J., Gruber-Khadjawi, M., Thallinger, G. G., Birner-Gruenberger, R., Gruber, K., Winkler, M. & Glieder, A., 26 May 2017, In : Scientific reports. 7, 46834.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
mandelonitrile lyase
Enzymes

Four distinct types of E.C. 1.2.1.30 enzymes can catalyze the reduction of carboxylic acids to aldehydes

Stolterfoht, H., Schwendenwein, D., Sensen, C. W., Rudroff, F. & Winkler, M., 2017, In : Journal of Biotechnology. 257, p. 222-232

Research output: Contribution to journalArticleResearchpeer-review

IN VIVO SYNTHESIS OF POLYHYDROXYLATED COMPOUNDS FROM A ‘HIDDEN RESERVOIR’ OF TOXIC ALDEHYDE SPECIES

Bayer, T., Wiesinger, T., Milker, S., Winkler, M., Mihovilovic, M. D. & Rudroff, F., 2017, In : ChemCatChem. 9, p. 2919-2923

Research output: Contribution to journalArticleResearchpeer-review

2016

Biocatalytic Characterization of Human FMO5: Unearthing Baeyer-Villiger Reactions in Humans

Fiorentini, F., Geier, M., Binda, C., Winkler, M., Faber, K., Hall, M. & Matteiv, A., 2016, In : ACS Chemical Biology. p. 1039 1048 p.

Research output: Contribution to journalArticleResearchpeer-review

Human xanthine oxidase recombinant in E. coli: A whole cell catalyst for preparative drug metabolite synthesis

Ferreira Antunes, M., Eggimann, F. K., Kittelmann, M., Lütz, S., Hanlon, S. P., Wirz, B., Bachler, T. & Winkler, M., 2016, In : Journal of Biotechnology. 235, p. 3-10

Research output: Contribution to journalArticleResearchpeer-review

Pichia pastoris mutants as host strains for efficient secretion of recombinant Branched Chain Aminotransferase (BCAT)

Weinhandl, K., Ballach, M., Winkler, M., Mudassar, A., Glieder, A., Birner-Gruenberger, R., Fotheringham, I. & Escalettes, F., 2016, In : Journal of Biotechnology. 235, p. 84-91 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Glycosylation
Pichia
Amino acids
Proteins
Amino Acids

Selective Enzymatic Transformation to Aldehydes in vivo by Fungal Carboxylate Reductase from Neurospora crassa

Schwendenwein, D., Fiume, G., Weber, H., Rudroff, F. & Winkler, M., 2016, In : Advanced Synthesis & Catalysis. 358, 21, p. 3414-3421

Research output: Contribution to journalArticleResearchpeer-review

TRAMETES VERSICOLOR CARBOXYLATE REDUCTASE UNCOVERED

Winkler, M. & Winkler, C. K., Mar 2016, In : Monatshefte für Chemie = Chemical monthly. 147, 3, p. 575-578

Research output: Contribution to journalArticleResearchpeer-review

2015

Bioprospecting for Hydroxynitrile Lyases by Blue Native PAGE Coupled HCN Detection

Lanfranchi, E., Köhler, E-M., Darnhofer, B., Steiner, K., Birner-Grünberger, R., Glieder, A. & Winkler, M., 2015, In : Current biotechnology. 4, 2, p. 111-117

Research output: Contribution to journalArticleResearchpeer-review

Drug Metabolite Synthesis with Human Enzymes

Winkler, M., 2015, In : International pharmaceutical industry. 7, 3, p. 32-36

Research output: Contribution to journalArticleResearch

FLUORINATED AMINO ACIDS – A RARITY IN NATURE YET A PROSPECT FOR PROTEIN ENGINEERING

Odar, C., Winkler, M. & Wiltschi, B., 2015, In : Biotechnology Journal. 10, 3, p. 427-446

Research output: Contribution to journalArticleResearchpeer-review

Human FMO2-based microbial whole-cell catalysts for drug metabolite synthesis

Geier, M., Bachler, T., Hanlon, S. P., Eggimann, F. K., Kittelmann, M., Weber, H., Lütz, S., Wirz, B. & Winkler, M., 2015, In : Microbial cell factories . 14, 82, p. 1-10

Research output: Contribution to journalArticleResearchpeer-review

The Nitrile forming Enzyme 7-Cyano-7-deazaguanine Synthase from Geobacillus kaustophilus: A reverse Nitrilase?

Winkler, M., Dokulil, K., Weber, H., Pavkov-Keller, T. & Wilding, B., 2015, In : ChemBioChem. 16, p. 2373-2378

Research output: Contribution to journalArticleResearchpeer-review

2014

BIOCATALYTIC REDUCTION OF CARBOXYLIC ACIDS

Napora, K., Strohmeier, G. & Winkler, M., 2014, In : Biotechnology Journal. 9, p. 822-843

Research output: Contribution to journalArticleResearchpeer-review

Carbon source dependent promoters in yeasts

Weinhandl, K., Winkler, M., Glieder, A. & Camattari, A., 2014, In : Microbial cell factories . 13, p. 5-21

Research output: Contribution to journalArticleResearchpeer-review

PRODUCTION OF RECOMBINANT HUMAN ALDEHYDE OXIDASE IN E. COLI AND OPTIMIZATION OF ITS APPLICATION FOR PREPARATIVE SYNTHESIS OF OXIDIZED DRUG METABOLITES

Rodrigues, D., Kittelmann, M., Eggimann, F., Bachler, T., Abad, S., Camattari, A., Glieder, A., Winkler, M. & Lütz, S., 2014, In : ChemCatChem. 6, 4, p. 1028-1042

Research output: Contribution to journalArticleResearchpeer-review

Whole cell carboxylate reduction for the synthesis of 3-hydroxytyrosol

Napora, K., Robins, K., Osorio-Lozada, A. & Winkler, M., 2014, In : ChemCatChem. 6, 4, p. 1089-1095

Research output: Contribution to journalArticleResearchpeer-review

2013

Enantiocomplementary Yarrowia lipolytica Oxidoreductases: Alcohol Dehydrogenase 2 and Short Chain Dehydrogenase/Reductase

Napora, K., Strohmeier, G., Sonavane, M. N., Avi, M., Robins, K. & Winkler, M., 2013, In : Biomolecules. 3, p. 449-460

Research output: Contribution to journalArticleResearchpeer-review

RECENT DEVELOPMENTS IN HYDROXYNITRILE LYASES FOR INDUSTRIAL BIOTECHNOLOGY

Lanfranchi, E., Steiner, K., Glieder, A., Hajnal, I., Sheldon, R. A., van Pelt, S. & Winkler, M., 2013, In : Recent patents on biotechnology. 7, 3, p. 197-206

Research output: Contribution to journalArticleResearchpeer-review

Targeting the Substrate Binding Site of E. coli Nitrile Reductase QueF by Modeling, Substrate and Enzyme Engineering

Wilding, B., Winkler, M., Petschacher, B., Kratzer, R., Egger, S., Steinkellner, G., Lyskowski, A. F., Nidetzky, B., Gruber, K. & Klempier, N., 2013, In : Chemistry - A European Journal. 19, 22, p. 7007-7012

Research output: Contribution to journalArticleResearchpeer-review

YARROWIA LIPOLYTICA DEHYDROGENASE/ REDUCTASE: AN ENZYME TOLERANT FOR LIPOPHILIC COMPOUNDS AND CARBOHYDRATE SUBSTRATES

Napora, K., Wrodnigg, T., Kosmus, P., Thonhofer, M., Robins, K. & Winkler, M., 2013, In : Bioorganic & Medicinal Chemistry Letters. 23, p. 3393-3395

Research output: Contribution to journalArticleResearchpeer-review

2012

A NOVEL MULTI-ENZYMATIC ASSAY FOR TRANSAMINASE ACTIVITY: HIGH THROUGHPUT SCREENING APPLICATIONS IN PICHIA PASTORIS

Weinhandl, K., Winkler, M., Glieder, A. & Camattari, A., 2012, In : Tetrahedron. 68, p. 7586-7590

Research output: Contribution to journalArticleResearchpeer-review

Expression of recombinant human flavin monooxygenase and moclobemide-N-oxide synthesis on multi-mg scale

Hanlon, S. P., Camattari, A., Abad, S., Glieder, A., Wirz, B., Kittelmann, M., Lütz, S. & Winkler, M., 2012, In : Chemical Communications. 48, 48, p. 6001-6003 3 p.

Research output: Contribution to journalArticleResearchpeer-review

Moclobemide
Mixed Function Oxygenases
Oxides
Drug Compounding
Escherichia coli

NITRILE REDUCTASE FROM GEOBACILLUS KAUSTOPHILUS: A POTENTIAL CATALYST FOR A NEW NITRILE BIOTRANSFORMATION REACTION

Wilding, B., Winkler, M., Kratzer, R., Petschacher, B., Glieder, A. & Klempier, N., 2012, In : Advanced Synthesis & Catalysis. 354, 11-12, p. 2191-2198

Research output: Contribution to journalArticleResearchpeer-review

Organ Function in Shake Flasks: Recombinant Human Enzymes for the Pharmaceutical Industry

Winkler, M., 2012, In : TU Graz research. 7, p. 32-35

Research output: Contribution to journalArticleResearch

2011

HIGH LEVEL EXPRESSION OF RHODOTORULA GRACILIS D-AMINO ACID OXIDASE IN PICHIA PASTORIS

Abad, S., Nahalka, J., Winkler, M., Bergler, G., Speight, R., Glieder, A. & Nidetzky, B., 2011, In : Biotechnology Letters. 33, 3, p. 557-563

Research output: Contribution to journalArticleResearchpeer-review

Old Yellow Enzyme catalyzed dehydrogenation of saturated ketones

Schittmayer, M., Glieder, A., Uhl, M. K., Winkler, A., Zach, S., Schrittwieser, J., Kroutil, W., Macheroux, P., Gruber, K., Kambourakis, S., Rozzell, J. D. & Winkler, M., 2011, In : Advanced Synthesis & Catalysis. 252, 2-3, p. 268-274

Research output: Contribution to journalArticleResearchpeer-review

2010

Directed evolution of Alcaligenes faecalis nitrilase

Schreiner, U., Hecher, B., Obrowsky, S., Waich, K., Klempier, N., Steinkellner, G., Gruber, K., Rozzell, J. D., Glieder, A. & Winkler, M., 2010, In : Enzyme and microbial technology. 47, p. 140-146

Research output: Contribution to journalArticleResearchpeer-review

IMPROVED FITNESS OF ARABIDOPSIS THALIANA NITRILASE 2

Schreiner, U., Steinkellner, G., Glieder, A., Rozzell, J. D. & Winkler, M., 2010, In : ChemCatChem. 2, 3, p. 263-267

Research output: Contribution to journalArticleResearchpeer-review

Monooxygenases as biocatalysts: classification, mechanistic aspects and biotechnological applications

Torres Pazmiño, D. E., Winkler, M., Glieder, A. & Fraaije, M. W., 2010, In : Journal of Biotechnology. 146, p. 9-24

Research output: Contribution to journalArticleResearchpeer-review

2009

A diversified library of bacterial und fungal bifunctional cytochrome P450 enzymes for drug metabolite synthesis

Weis, R., Winkler, M., Schittmayer, M., Kambourakis, S., Vink, M., Rozzell, D. & Glieder, A., 2009, In : Advanced Synthesis & Catalysis. 13, 351, p. 2140-2146

Research output: Contribution to journalArticleResearchpeer-review

BIOCATALYTIC APPLICATION OF NITRILASES FROM FUSARIUM SOLANI O1 AND ASPERGILLUS NIGER K10

Winkler, M., Kaplan, O., Vejvoda, V., Klempier, N. & Martinkova, L., 2009, In : Journal of molecular catalysis / B. 59, p. 243-247

Research output: Contribution to journalArticleResearchpeer-review

ENANTIOSEPARATION OF NONPROTEINOGENIC AMINO ACIDS

Winkler, M. & Klempier, N., 2009, In : Analytical and Bioanalytical Chemistry. 393, p. 1789-1796

Research output: Contribution to journalArticleResearchpeer-review

Enantioseparation of Non-proteinogenic Amino Acids

Klempier, N. & Winkler, M., 2009, In : Analytical and Bioanalytical Chemistry. 393, 6, p. 1789-1796

Research output: Contribution to journalArticleResearchpeer-review

Synthesis and Vanilloid Receptor (TRPV1) Activity of the Enantiomers of α-Fluorinated Capsaicin

Winkler, M., Moraux, T., Khairy, H. A., Scott, R. H., Slawin, A. M. Z. & O'Hagan, D., 2009, In : ChemBioChem. 10, 5, p. 823-828

Research output: Contribution to journalArticleResearchpeer-review

The Fluorinase Enzyme as a Synthetic Tool for the Synthesis of new Fluorine-18 Labelled Sugars for Positron Emission Tomography

Onega, M., Winkler, M. & O'Hagan, D., 2009, In : Future medicinal chemistry. 1, 5, p. 865-873

Research output: Contribution to journalArticleResearchpeer-review