Harald Pichler

Assoc.Prof. Dipl.-Ing. Dr.techn.

19962019
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Research Output 1996 2019

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Article
2018

Modification of Membrane Lipid Compositions in single-celled organisms–from Basics to Applications

Pichler, H. & Emmerstorfer-Augustin, A., 13 Sep 2018, In : Methods. 147, p. 50 - 65 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

On the current role of hydratases in biocatalysis

Engleder, M. & Pichler, H., 15 Jul 2018, In : Applied Microbiology and Biotechnology. 102, p. 5841-5858

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Perdeuteration of cholesterol for neutron scattering applicatioins using recombinant Pichia pastoris

Mouline, M., Strohmeier, G., Hirz, M., Thompson, K., Rennie, A., Campbell, R. A., Pichler, H., Maric, S., Forsyth, T. & Haertlein, M., 5 Feb 2018, In : Chemistry and Physics of Lipids. 212, p. 80-87

Research output: Contribution to journalArticleResearchpeer-review

Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca

Engleder, M., Horvat, M., Emmerstorfer-Augustin, A., Wriessnegger, T., Gabriel, S., Strohmeier, G., Weber, H., Müller, M., Kaluzna, I., Mink, D., Schürmann, M. & Pichler, H., 8 Feb 2018, In : PLoS ONE.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

The Impact of Deuteration on Natural and Synthetic Lipids: a Neutron Diffraction Study

Luchini, A., Delhom, R., Demé, B., Laux, V., Mouline, M., Haertlein, M., Pichler, H., Strohmeier, G., Wacklin, H. & Fragneto, G., 1 Aug 2018, In : Colloids and Surfaces / B. p. 126 - 133

Research output: Contribution to journalArticleResearchpeer-review

Whole-cell (+)-ambrein production in the yeast Pichia pastoris

Moser, S., Strohmeier, G., Leitner, E., Plocek, T. J., Vanhessche, K. & Pichler, H., Dec 2018, In : Metabolic Engineering Communications. 7, 9 p., e00077.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
2017

Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine

Waldie, S., Lind, T. K., Browning, K., Mouline, M., Haertlein, M., Forsyth, T., Luchini, A., Strohmeier, G., Pichler, H., Maric, S. & Cárdenas, M., 27 Dec 2017, In : Langmuir. 34, 1, p. 472-479 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipid bilayers
Cholesterol
cholesterol
Lipid Bilayers
Phosphatidylcholines
2016

Enhancing cytochrome P450-mediated conversions in P. pastoris through RAD52 over-expression and optimizing the cultivation conditions

Wriessnegger, T., Moser, S., Emmerstorfer-Augustin, A., Leitner, E., Müller, M., Kaluzna, I., Schürmann, M., Daniel, M. & Pichler, H., 8 Mar 2016, In : Fungal genetics and biology. 89, p. 114-125

Research output: Contribution to journalArticleResearchpeer-review

Screening for improved isoprenoid biosynthesis in microorganisms

Emmerstorfer, A., Moser, S. & Pichler, H., 2016, In : Journal of Biotechnology. 235, p. 112–120

Research output: Contribution to journalArticleResearchpeer-review

2015

Over-expression of ICE2 stabilizes cytochrome P450 reductase levels in Saccharomyces cerevisiae and Pichia pastoris

Emmerstorfer, A., Wimmer-Teubenbacher, M., Wriessnegger, T., Leitner, E., Müller, M., Kaluzna, I., Schürmann, M., Mink, D., Zellnig, G., Schwab, H. & Pichler, H., 2015, In : Biotechnology Journal. 10, p. 623-635

Research output: Contribution to journalArticleResearchpeer-review

Sphingolipids regulate telomere clustering by affecting transcriptional levels of genes involved in telomere homeostasis

Ikeda, A., Muneoka, T., Murakami, S., Hirota, A., Yabuki, Y., Karashima, T., Nakazono, K., Tsuruno, M., Pichler, H., Shirahige, K., Kodama, Y., Shimamoto, T., Mizuta, K. & Funato, K., 2015, In : Journal of cell science. 128, p. 2454-2467

Research output: Contribution to journalArticleResearchpeer-review

Structure-based mechanism of oleate hydratase from Elizabethkingia meningoseptica

Engleder, M., Pavkov-Keller, T., Emmerstorfer, A., Altijana, H., Schrempf, S., Steinkellner, G., Wriessnegger, T., Leitner, E., Strohmeier, G., Kaluzna, I., Daniel, M., Martin, S., Wallner, S., Macheroux, P., Gruber, K. & Pichler, H., 2015, In : ChemBioChem. 16, p. 1730-1734

Research output: Contribution to journalArticleResearchpeer-review

2014

Overexpression of membrane proteins from higher eukaryotes in yeasts

Emmerstorfer, A., Wriessnegger, T., Hirz, M. & Pichler, H., 2014, In : Applied Microbiology and Biotechnology. 98, 18, p. 7671-7698

Research output: Contribution to journalArticleResearchpeer-review

Pichia pastoris Aft1 - a novel transcription factor, enhancing recombinant protein secretion

Ruth, C., Buchetics, M., Vidimce, V., Kotz, D., Naschberger, S., Mattanovich, D., Pichler, H. & Gasser, B., 2014, In : Microbial cell factories . 13, p. 120-120

Research output: Contribution to journalArticleResearchpeer-review

Production of the sesquiterpenoid (+)-nootkatone by metabolic engineering of Pichia pastors

Wriessnegger, T., Augustin, P. A., Engleder, M., Leitner, E., Müller, M., Kaluzna, I., Schürmann, M., Mink, D., Zellnig, G., Schwab, H. & Pichler, H., 2014, In : Metabolic engineering. 24, p. 18-29

Research output: Contribution to journalArticleResearchpeer-review

Protein expression in Pichia pastoris: recent achievements and perspectives for heterologous protein production

Mudassar, A., Hirz, M., Pichler, H. & Schwab, H., 2014, In : Applied Microbiology and Biotechnology. 98, p. 5301-5317

Research output: Contribution to journalArticleResearchpeer-review

2013

Enzymatic aerobic alkene cleavage catalyzed by a Mn3+-dependent proteinase A homologue

Rajagopalan, A., Schober, M., Emmerstorfer, A., Hammerer, L., Migglautsch, A., Seisser, B., Glück, S. M., Niehaus, F., Eck, J., Pichler, H., Gruber, K. & Kroutil, W., 2013, In : ChemBioChem. 14, p. 2427-2430

Research output: Contribution to journalArticleResearchpeer-review

Yeast metabolic engineering - targeting sterol metabolism and terpenoid formation

Wriessnegger, T. & Pichler, H., 2013, In : Progress in lipid research. 52, p. 277-293

Research output: Contribution to journalArticleResearchpeer-review

2012

Production of human cytochrome P450 2D6 drug metabolites with recombinant microbes--a comparative study

Geier, M., Braun, A., Emmerstorfer, A., Pichler, H. & Glieder, A., 2012, In : Biotechnology Journal. 7, 11, p. 1346-1358 13 p.

Research output: Contribution to journalArticleResearchpeer-review

Cytochrome P-450 CYP2D6
Enzymes
Pharmaceutical Preparations
Pichia
Biotransformation
2011

Application of Designed Enzymes in Organic Synthesis

Strohmeier, G., Pichler, H., May, O. & Gruber, M., 2011, In : Chemical Reviews. 111, p. 4141-4164

Research output: Contribution to journalArticleResearchpeer-review

A stable yeast strain efficiently producing cholesterol instead of ergosterol is functional for tryptophan uptake, but not weak organic acid resistance

Souza, C. M., Schwabe, T. M., Pichler, H., Ploier, B., Leitner, E., Guan, X. L., Wenk, M. W., Riezman, I. & Riezman, H., 2011, In : Metabolic engineering. 13, p. 555-569

Research output: Contribution to journalArticleResearchpeer-review

High-quality genome sequence of Pichia pastoris CBS7435

Küberl, A., Schneider, J., Thallinger, G., Anderl, I., Wibberg, D., Hajek, T., Jaenicke, S., Brinkrolf, K., Goesmann, A., Szczepanowski, R., Pühler, A., Schwab, H., Glieder, A. & Pichler, H., 2011, In : Journal of Biotechnology. 154, p. 312-320

Research output: Contribution to journalArticleResearchpeer-review

2009

Functional interactions between sphingolipids and sterols in biological membranes regulating cell physiology

Guan, X., Souza, C. M., Pichler, H., Dewhurst, G., Schaad, O., Kajiwara, K., Wakabayashi, H., Ivanova, T., Castillon, G. A., Piccolis, M., Abe, F., Loewith, R., Funato, K., Wenk, M. R. & Riezman, H., 2009, In : Molecular biology of the cell. 20, p. 2083-2095

Research output: Contribution to journalArticleResearchpeer-review

Tuning microbial hosts for membrane protein production

Freigaßner, M. E., Pichler, H. & Glieder, A., 2009, In : Microbial cell factories . 8, p. 69-.

Research output: Contribution to journalArticleResearchpeer-review

2008

Alternative pig liver esterase (APLE) - Cloning, identification and functional expression in Pichia pastoris of a versatile new biocatalyst

Hermann, M., Kietzmann, M. U., Ivancic, M., Zenzmaier, C., Luiten, R., Skranc, W., Wubbolts, M., Winkler, M., Birner-Grünberger, R., Pichler, H. & Schwab, H., 2008, In : Journal of Biotechnology. 133, p. 301-310

Research output: Contribution to journalArticleResearchpeer-review

Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the ER

Kajiwara, K., Watanabe, R., Pichler, H., Ihara, K., Murakami, S., Riezman, H. & Funato, K., 2008, In : Molecular biology of the cell. 19, p. 2069-2082

Research output: Contribution to journalArticleResearchpeer-review

2007

Lipid requirements for endocytosis in yeast

Souza, C. M. & Pichler, H., 2007, In : Biochimica et Biophysica Acta. 1771, p. 442-454

Research output: Contribution to journalArticleResearchpeer-review

2005

Multiple lipid transport pathways to the plasma membrane in yeast

Schnabl, M., Daum, G. & Pichler, H., 2005, In : Biochimica et Biophysica Acta. 1687, p. 130-140

Research output: Contribution to journalArticleResearchpeer-review

2004

Where sterols are required for endocytosis

Pichler, H. & Riezman, H., 2004, In : Biochimica et Biophysica Acta. 1666, p. 51-61

Research output: Contribution to journalArticleResearchpeer-review

2003

Subcellular localization of yeast Sec14 homologues and their involvement in regulation of phospholipid turnover

Schnabl, M., Oskolkova, O., Holic, R., Brezna, B., Pichler, H., Zagorsek, M., Kohlwein, S. D., Paltauf, F., Daum, G. & Griac, P., 2003, In : European journal of biochemistry. 270, p. 3133-3145

Research output: Contribution to journalArticleResearchpeer-review

2002

Multiple functions of sterols in yeast endocytosis

Heese-Peck, A., Pichler, H., Zanolari, B., Watanabe, R., Daum, G. & Riezman, H., 2002, In : Molecular biology of the cell. 13, p. 2664-2680

Research output: Contribution to journalArticleResearchpeer-review

2001

A subfraction of the yeast endoplasmic reticulum associates with the plasma membrane and has a high capacity to synthesize lipids

Pichler, H., Gaigg, B., Hrastnik, C., Achleitner, G., Kohlwein, S-D., Zellnig, G., Perktold, A. & Daum, G., 2001, In : European journal of biochemistry. 268, 8, p. 2351-2361

Research output: Contribution to journalArticleResearchpeer-review

1999

PDR16 and PDR17, two homologous genes of Saccharomyces cerevisiae, affect lipid biosynthesis and resistance to multiple drugs

Van den Hazel, H. B., Pichler, H., do Valle Matta, M. A., Leitner, E., Goffeau, A. & Daum, G., 1999, In : The Journal of Biological Chemistry. 274, p. 1934-1941

Research output: Contribution to journalArticleResearchpeer-review

Specific sterols required for the internalization step of endocytosis in yeast

Munn, A. L., Heese-Peck, A., Stevenson, B. J., Pichler, H. & Riezman, H., 1999, In : Molecular biology of the cell. 10, p. 3943-3957

Research output: Contribution to journalArticleResearchpeer-review