Bernd Nidetzky

2019

Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, In : Catalysis Science & Technology. 9, 3, p. 842-853 12 p.

Research output: Contribution to journal › Article › Research › peer-review

Open Access

Nitriles

Proton transfer

Catalysis

Escherichia coli

Protons

2018

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

Valikhani, D., Bolivar, J. M., Dennig, A. & Nidetzky, B., 1 Oct 2018, In : Biotechnology and Bioengineering. 115, 10, p. 2416-2425 10 p.

Research output: Contribution to journal › Article › Research › peer-review

Glucose 1-Dehydrogenase

Mixed Function Oxygenases

Cytochrome P-450 Enzyme System

Bacillus megaterium

Glucose

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisán, J. M. & Nidetzky, B., 28 Feb 2018, In : ACS Applied Materials & Interfaces. 10, 8, p. 6858-6868 11 p.

Research output: Contribution to journal › Article › Research › peer-review

Optical sensors

Proteins

pH sensors

Penicillin Amidase

Immobilized Enzymes

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

Research output: Contribution to journal › Article › Research › peer-review

decarboxylation

Carboxy-Lyases

fatty acids

Hydrocarbons

Fatty acids

Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from Lipomyces starkeyi

Rother, C., Gutmann, A., Gudiminchi, R., Weber, H., Lepak, A. & Nidetzky, B., 16 Mar 2018, In : ChemBioChem. 19, 6, p. 596-603 8 p.

Research output: Contribution to journal › Article › Research › peer-review

Lipomyces

Glucose-6-Phosphate

Phosphotransferases

Adenosine Diphosphate

Adenosine Triphosphate

Demystifying the Flow: Biocatalytic Reaction Intensification in Microstructured Enzyme Reactors

Bolivar, J. M., Valikhani, D. & Nidetzky, B., 9 Aug 2018, In : Biotechnology Journal.

Research output: Contribution to journal › Article › Research › peer-review

Immobilized Enzymes

sucrose phosphorylase

Enzymes

Biocatalysis

Biotransformation

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

Jung, J. & Nidetzky, B., 1 Jan 2018, In : Journal of Biological Chemistry. 293, 10, p. 3720-3733 14 p.

Research output: Contribution to journal › Article › Research › peer-review

Nitriles

Imines

Escherichia coli

Amines

Oxidoreductases

Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

Schmölzer, K., Lemmerer, M. & Nidetzky, B., 1 Mar 2018, In : Biotechnology and Bioengineering. 115, 3, p. 545-556 12 p.

Research output: Contribution to journal › Article › Research › peer-review

Glycosyltransferases

Glucosides

Polyphenols

Phloretin

Glycosylation

l-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: Aeration and carbon source influence yields and productivities

Novy, V., Brunner, B. & Nidetzky, B., 11 Apr 2018, In : Microbial cell factories . 17, 1, 59.

Research output: Contribution to journal › Article › Research › peer-review

Xylose

Lactic acid

Yeast

Glucose

Saccharomyces cerevisiae

Modeling the activity burst in the initial phase of cellulose hydrolysis by the processive cellobiohydrolase Cel7A

Petrášek, Z., Eibinger, M. & Nidetzky, B., 4 Dec 2018, In : Biotechnology and Bioengineering.

Research output: Contribution to journal › Article › Research › peer-review

Cellulose 1,4-beta-Cellobiosidase

Cellulose

Hydrolysis

Cellulases

Enzymes

New flavanol O-glycosides in grape and wine

Zerbib, M., Mazauric, J. P., Meudec, E., Le Guernevé, C., Lepak, A., Nidetzky, B., Cheynier, V., Terrier, N. & Saucier, C., 15 Nov 2018, In : Food chemistry. 266, p. 441-448 8 p.

Research output: Contribution to journal › Article › Research › peer-review

Wine

Catechin

Vitis

flavanols

Glycosides

Process intensification for O₂-dependent enzymatic transformations in continuous single-phase pressurized flow

Bolivar, J. M., Mannsberger, A., Thomsen, M. S., Tekautz, G. & Nidetzky, B., 4 Dec 2018, In : Biotechnology and Bioengineering.

Research output: Contribution to journal › Article › Research › peer-review

Oxidoreductases

Biotransformation

Catalase

Pressure

Glucose Oxidase

Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

Benítez-Mateos, A. I., Nidetzky, B., Bolivar, J. M. & López-Gallego, F., 21 Feb 2018, In : ChemCatChem. 10, 4, p. 654-665 12 p.

Research output: Contribution to journal › Article › Research › peer-review

Biocatalysts

Immobilized Enzymes

Enzymes

enzymes

Structural integrity

Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Escobar, S., Bernal, C., Bolivar, J. M., Nidetzky, B., López-Gallego, F. & Mesa, M., 1 Apr 2018, In : Molecular Catalysis. p. 106-113 8 p.

Research output: Contribution to journal › Article › Research › peer-review

Polyethyleneimine

Lipases

Lipase

Encapsulation

Silicon Dioxide

β-Glucosyl Fluoride as Reverse Reaction Donor Substrate and Mechanistic Probe of Inverting Sugar Nucleotide-Dependent Glycosyltransferases

Lepak, A., Gutmann, A. & Nidetzky, B., 5 Oct 2018, In : ACS Catalysis. 8, 10, p. 9148-9153 6 p.

Research output: Contribution to journal › Article › Research › peer-review

Flavonoids

Glycosyltransferases

Diphosphates

Uridine

Nucleotides

2017

An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

Gutmann, A., Schiller, M., Gruber-Khadjawi, M. & Nidetzky, B., 5 Sep 2017, In : Organic & biomolecular chemistry. 15, p. 7917 7924 p.

Research output: Contribution to journal › Article › Research › peer-review

A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

Valikhani, D., Bolivar, J. M., Viefhues, M., McIlroy, D. N., Vrouwe, E. X. & Nidetzky, B., 11 Oct 2017, In : ACS Applied Materials & Interfaces. 9, 40, p. 34641-34649 9 p.

Research output: Contribution to journal › Article › Research › peer-review

Enzyme immobilization

Microfluidics

Silicon Dioxide

Enzymes

Silica

Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose

Kulmer, S. T., Gutmann, A., Lemmerer, M. & Nidetzky, B., 2017, In : Advanced Synthesis & Catalysis. 359, p. 292-301

Research output: Contribution to journal › Article › Research › peer-review

Open Access

Sugar (sucrose)

Nucleotides

Nucleosides

Glucose

Diphosphates

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

Research output: Contribution to journal › Article › Research › peer-review

Uridine Diphosphate Glucose

Glycosyltransferases

Uridine Diphosphate

Recycling

Biological Products

Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation

Leis, D., Lauß, B., Macher-Ambrosch, R., Andreas, P., Nidetzky, B. & Kratzer, R., 2017, In : Journal of Biotechnology. 257, p. 110-117

Research output: Contribution to journal › Article › Research › peer-review

Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie / International Edition . 56, p. 2503 –2507

Research output: Contribution to journal › Article › Research › peer-review

Xylose

Uridine Diphosphate

Isotopes

Enzymes

Sugars

Multivalency Effects on the Immobilization of Sucrose Phosphorylase in Flow Microchannels and Their Use in the Development of a High-Performance Biocatalytic Microreactor

Valikhani, D., Bolivar, J. M., Pfeiffer, M. & Nidetzky, B., 9 Jan 2017, In : ChemCatChem. 9, 1, p. 161-166 6 p.

Research output: Contribution to journal › Article › Research › peer-review

sucrose phosphorylase

sucrose

Sugar (sucrose)

microchannels

Microchannels

Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

Bolivar, J. M., Luley-Goedl, C., Leitner, E., Sawangwan, T. & Nidetzky, B., 10 Sep 2017, In : Journal of Biotechnology. 257, p. 131-138 8 p.

Research output: Contribution to journal › Article › Research › peer-review

sucrose phosphorylase

Sugar (sucrose)

Glycerol

Catalyst supports

Enzymes

Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie. 129, p. 2544 –2548

Research output: Contribution to journal › Article › Research › peer-review

Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

Diricks, M., Gutmann, A., Debacker, S., Dewitte, G., Nidetzky, B. & Desmet, T., 2017, In : Protein engineering, design and selection. 30, 3, p. 141-148

Research output: Contribution to journal › Article › Research › peer-review

Uridine Diphosphate

Sugar (sucrose)

Nucleotides

Administrative data processing

Diphosphates

Single-molecule study of oxidative enzymaticdeconstruction of cellulose

Eibinger, M., Sattelkow, J., Ganner, T., Plank, H. & Nidetzky, B., 2017, In : Nature Communications . p. 8:894 7 p.

Research output: Contribution to journal › Article › Research

Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors

Viefhues, M., Sun, S., Valikhani, D., Nidetzky, B., Vrouwe, E. X., Mayr, T. & Bolivar, J. M., 10 May 2017, In : Journal of Micromechanics and Microengineering. 27, 6, 065012.

Research output: Contribution to journal › Article › Research › peer-review

Oxygen sensors

Immobilized Enzymes

Glucose Oxidase

Glucose oxidase

Optical sensors

Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l-lactate dehydrogenase within pdc1-pdc5 deletion background

Novy, V., Brunner, B., Müller, G. & Nidetzky, B., 1 Jan 2017, In : Biotechnology and Bioengineering. 114, 1, p. 163-171 9 p.

Research output: Contribution to journal › Article › Research › peer-review

Xylose

L-Lactate Dehydrogenase

Yeast

Fermentation

Glucose

Walking a Fine Line with Sucrose Phosphorylase: Efficient Single-Step Biocatalytic Production of l-Ascorbic Acid 2-Glucoside from Sucrose

Gudiminchi, R. K. & Nidetzky, B., 18 Jul 2017, In : ChemBioChem. 18, 14, p. 1387-1390 4 p.

Research output: Contribution to journal › Article › Research › peer-review

sucrose phosphorylase

Ascorbic Acid

Sucrose

Cosmetics

Protonation

2016

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

Bolivar, J. M., Eisl, I. & Nidetzky, B., 1 Jan 2016, In : Catalysis Today. 259, p. 66-80 15 p.

Research output: Contribution to journal › Article › Research › peer-review

Immobilized Enzymes

Biocatalysts

Enzymes

Catalyst supports

Proteins

Biotechnological production of fucosylated human milk oligosaccharides: Prokaryotic fucosyltransferases and their use in biocatalytic cascades or whole cell conversion systems.

Petschacher, B. & Nidetzky, B., 1 Apr 2016, In : Journal of Biotechnology. 235, p. 61-83 23 p.

Research output: Contribution to journal › Article › Research › peer-review

Fucosyltransferases

Oligosaccharides

Human Milk

galactoside 3-fucosyltransferase

Guanosine Diphosphate Fucose

Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution

Eibinger, M., Zahel, T., Ganner, T., Plank, H. & Nidetzky, B., 8 Mar 2016, In : Biotechnology for Biofuels . 9, 56

Research output: Contribution to journal › Article › Research › peer-review

Development of a fully integrated falling film microreactor for gas-liquid-solid biotransformation with surface immobilized O₂-dependent enzyme

Bolivar Bolivar, J. M., Krämer, C. E. M., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering.

Research output: Contribution to journal › Article › Research › peer-review

Biotransformation

Enzymes

Gases

Immobilization

Oxidoreductases

Direct-Write Fabrication of Cellulose Nano-Structures via Focused Electron Beam Induced Nanosynthesis

Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2 Sep 2016, In : Scientific reports. p. 1-11 11 p.

Research output: Contribution to journal › Article › Research › peer-review

Downstream Processing of Nucleoside-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption

Lemmerer, M., Schmoelzer, K., Gutmann, A. & Nidetzky, B., 2016, In : Advanced Synthesis & Catalysis. 358, p. 3113-3122

Research output: Contribution to journal › Article › Research › peer-review

Sugar (sucrose)

Nucleosides

Sugars

Chromatography

Anions

Functional characterization of thenative swollenin from Trichoderma reesei:study of its possible role as C1 factor ofenzymatic lignocellulose conversion

Eibinger, M., Sigl, K., Sattelkow, J., Ganner, T., Ramoni, J., Seiboth, B., Plank, H. & Nidetzky, B., 2016, In : Biotechnology for Biofuels . p. 9:178

Research output: Contribution to journal › Article › Research › peer-review

Kinetic analysis and probing with substrate analogues of the reaction pathway of the nitrile reductase QueF from Escherichia coli

Jung, J., Czabany, T., Wilding, B., Klempier, N. & Nidetzky, B., 2016, In : The Journal of Biological Chemistry. 291, 49, p. 25411-25426 16 p.

Research output: Contribution to journal › Article › Research › peer-review

Nitriles

Escherichia coli

Oxidoreductases

Kinetics

Substrates

Let the substrate flow, not the enzyme: Practical immobilization of d-amino acid oxidase in a glass microreactor for effective biocatalytic conversions

Bolivar Bolivar, J. M., Tribulato, M. A., Petrasek, Z. & Nidetzky, B., 1 Nov 2016, In : Biotechnology and Bioengineering. 113, 11, p. 2342-2349 8 p.

Research output: Contribution to journal › Article › Research › peer-review

Enzyme immobilization

Immobilization

Glass

Amino acids

Oxidoreductases

Screening of recombinant glycosyltransferases reveals the broad acceptor specificity of stevia UGT-76G1

Dewitte, G., Walmagh, M., Diricks, M., Lepak, A., Gutmann, A., Nidetzky, B. & Desmet, T., 1 Jun 2016, In : Journal of Biotechnology. 233, p. 49-55

Research output: Contribution to journal › Article › Research › peer-review

Stevia

Glycosylation

Glycosyltransferases

Uridine Diphosphate

Glycosides

Special Issue on acib, Dedicated to the Occasion of Prof. Dr. Helmut Schwab's 65th Birthday

Nidetzky, B. & Sensen, C. W., 14 Jul 2016, In : Journal of Biotechnology.

Research output: Contribution to journal › Article › Research

Sucrose synthase: A unique glycosyltransferase for biocatalytic glycosylation process development

Schmoelzer, K., Gutmann, A., Diricks, M., Desmet, T. & Nidetzky, B., 2016, In : Biotechnology Advances. 34, 2, p. 88-111

Research output: Contribution to journal › Article › Research › peer-review

Glycosylation

Glycosyltransferases

Diphosphates

Sugar (sucrose)

Nucleosides

The micromorphology of Trichoderma reesei analyzed in cultivations on lactose and solid lignocellulosic substrate, and its relationship with cellulase production

Novy, V., Schmid, M., Eibinger, M., Petrasek, Z. & Nidetzky, B., 9 Aug 2016, In : Biotechnology for Biofuels. 9, 1, 169.

Research output: Contribution to journal › Article › Research › peer-review

Trichoderma

micromorphology

Cellulase

Lactose

Enzymes

Unlocking the Potential of Leloir Glycosyltransferases for Applied Biocatalysis: Efficient Synthesis of Uridine 5′-Diphosphate-Glucose by Sucrose Synthase

Gutmann, A. & Nidetzky, B., 25 Oct 2016, In : Advanced Synthesis & Catalysis. 358, p. 3600-3609

Research output: Contribution to journal › Article › Research › peer-review

Glycosyltransferases

Diphosphates

Uridine

Sugar (sucrose)

Glucose

β-Cyclodextrin Improves Solubility and Enzymatic C-Glucosylation of the Flavonoid Phloretin

Bungaruang, L., Gutmann, A. & Nidetzky, B., 2016, In : Advanced Synthesis & Catalysis. 358, 3, p. 486-493

Research output: Contribution to journal › Article › Research › peer-review

Phloretin

Flavonoids

Cyclodextrins

Solubility

Complexation

2015

All-in-one assay for β-D-galactoside sialyltransferases: quantification of productive turnover, error hydrolysis and site selectivity

Czabany, T., Schmoelzer, K., Luley, C., Ribitsch, D. & Nidetzky, B., 2015, In : Analytical biochemistry. 483, p. 47-53

Research output: Contribution to journal › Article › Research › peer-review

Comparison of broad-scope assays of nucleotide sugar-dependent glycosyltransferases.

Czabany, T., Bubner, P., Luley, C. & Nidetzky, B., 2015, In : Analytical biochemistry. 490, p. 46-51

Research output: Contribution to journal › Article › Research › peer-review

Complete switch from α-2,3- to α-2,6-regioselectivity in Pasteurella dagmatis β-D-galactoside sialyltransferase by active-site redesign

Schmoelzer, K., Czabany, T., Luley, C., Pavkov-Keller, T., Ribitsch, D., Schwab, H., Gruber, K., Weber, H. & Nidetzky, B., 2015, In : Chemical Communications. p. 3083-3086

Research output: Contribution to journal › Article › Research › peer-review

Creating a Water-Soluble Resveratrol-Based Antioxidant by Site-Selective Enzymatic Glucosylation

Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 2015, In : ChemBioChem. 16, 13, p. 1870-1874

Research output: Contribution to journal › Article › Research › peer-review

Antioxidants

Water

Solubility

Glycosylation

Glucosyltransferases

Diastereoselective Synthesis of Glycosyl Phosphates by Using a Phosphorylase-Phosphatase Combination Catalyst

Wildberger, P., Pfeiffer, M., Brecker, L. & Nidetzky, B., 21 Dec 2015, In : Angewandte Chemie / International Edition . 54, 52, p. 15867-15871 5 p.

Research output: Contribution to journal › Article › Research › peer-review

Phosphorylase Phosphatase

Phosphatases

Phosphates

Phosphorylation

Catalysts

Identification of novel metabolic interactions controlling carbon flux from xylose to ethanol in natural and recombinant yeasts

Trausinger, G., Gruber, C., Krahulec, S., Magnes, C., Nidetzky, B. & Klimacek, M., 2015, In : Biotechnology for Biofuels . 8:157, p. 1-13

Research output: Contribution to journal › Article › Research › peer-review