Bernd Nidetzky

Univ.-Prof. Dipl.-Ing. Dr.techn.

1966 …2023
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Research Output 1966 2019

2019

Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry

Hengge, E., Steyskal, E-M., Bachler, R., Dennig, A., Nidetzky, B. & Würschum, R., 18 Nov 2019, In : Beilstein Journal of Nanotechnology . 10, p. 2275-2279 5 p.

Research output: Contribution to journalLetterResearchpeer-review

Open Access
cysteine
Gold
Cysteine
Monolayers
Desorption

Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, In : Catalysis Science & Technology. 9, 3, p. 842-853 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Nitriles
Proton transfer
Catalysis
Escherichia coli
Protons
2018

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

Valikhani, D., Bolivar, J. M., Dennig, A. & Nidetzky, B., 1 Oct 2018, In : Biotechnology and Bioengineering. 115, 10, p. 2416-2425 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucose 1-Dehydrogenase
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Bacillus megaterium
Glucose

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisán, J. M. & Nidetzky, B., 28 Feb 2018, In : ACS Applied Materials & Interfaces. 10, 8, p. 6858-6868 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Optical sensors
Proteins
pH sensors
Penicillin Amidase
Immobilized Enzymes

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

Research output: Contribution to journalArticleResearchpeer-review

decarboxylation
Carboxy-Lyases
fatty acids
Hydrocarbons
Fatty acids

Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from Lipomyces starkeyi

Rother, C., Gutmann, A., Gudiminchi, R., Weber, H., Lepak, A. & Nidetzky, B., 16 Mar 2018, In : ChemBioChem. 19, 6, p. 596-603 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Glucose-6-Phosphate
Phosphotransferases
Adenosine Diphosphate
Adenosine Triphosphate

Demystifying the Flow: Biocatalytic Reaction Intensification in Microstructured Enzyme Reactors

Bolivar, J. M., Valikhani, D. & Nidetzky, B., 9 Aug 2018, In : Biotechnology Journal.

Research output: Contribution to journalArticleResearchpeer-review

Immobilized Enzymes
sucrose phosphorylase
Enzymes
Biocatalysis
Biotransformation

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

Jung, J. & Nidetzky, B., 1 Jan 2018, In : Journal of Biological Chemistry. 293, 10, p. 3720-3733 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Nitriles
Imines
Escherichia coli
Amines
Oxidoreductases

Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

Schmölzer, K., Lemmerer, M. & Nidetzky, B., 1 Mar 2018, In : Biotechnology and Bioengineering. 115, 3, p. 545-556 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Glycosyltransferases
Glucosides
Polyphenols
Phloretin
Glycosylation

Humane Enzyme für die Organische Synthese

Winkler, M., Geier, M., Hanlon, S. P., Nidetzky, B. & Glieder, A., 2018, In : Angewandte Chemie. 130, p. 13592-13610

Research output: Contribution to journalReview articleResearchpeer-review

Human Enzymes for Organic Synthesis

Winkler, M., Geier, M., Hanlon, S. P., Nidetzky, B. & Glieder, A., 2018, In : Angewandte Chemie / International Edition . 57, 41, p. 13406-13423 18 p.

Research output: Contribution to journalReview articleResearchpeer-review

Enzymes
Pharmaceutical Preparations
Xenobiotics
Metabolites
Metabolism

Leloir Glycosyltransferases as Biocatalysts for Chemical Production

Nidetzky, B., Gutmann, A. & Zhong, C., 6 Jul 2018, In : ACS Catalysis. 8, 7, p. 6283-6300 18 p.

Research output: Contribution to journalReview articleResearchpeer-review

Glycosylation
Glycosyltransferases
Biocatalysts
Enzymes
Nucleotides

l-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: Aeration and carbon source influence yields and productivities

Novy, V., Brunner, B. & Nidetzky, B., 11 Apr 2018, In : Microbial cell factories . 17, 1, 59.

Research output: Contribution to journalArticleResearchpeer-review

Xylose
Lactic acid
Yeast
Glucose
Saccharomyces cerevisiae
Cellulose 1,4-beta-Cellobiosidase
Cellulose
Hydrolysis
Cellulases
Enzymes

New flavanol O-glycosides in grape and wine

Zerbib, M., Mazauric, J. P., Meudec, E., Le Guernevé, C., Lepak, A., Nidetzky, B., Cheynier, V., Terrier, N. & Saucier, C., 15 Nov 2018, In : Food chemistry. 266, p. 441-448 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Catechin
Vitis
flavanols
Glycosides

Process intensification for O2-dependent enzymatic transformations in continuous single-phase pressurized flow

Bolivar, J. M., Mannsberger, A., Thomsen, M. S., Tekautz, G. & Nidetzky, B., 4 Dec 2018, In : Biotechnology and Bioengineering.

Research output: Contribution to journalArticleResearchpeer-review

Oxidoreductases
Biotransformation
Catalase
Pressure
Glucose Oxidase

Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

Benítez-Mateos, A. I., Nidetzky, B., Bolivar, J. M. & López-Gallego, F., 21 Feb 2018, In : ChemCatChem. 10, 4, p. 654-665 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Biocatalysts
Immobilized Enzymes
Enzymes
enzymes
Structural integrity

Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Escobar, S., Bernal, C., Bolivar, J. M., Nidetzky, B., López-Gallego, F. & Mesa, M., 1 Apr 2018, In : Molecular Catalysis. p. 106-113 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Polyethyleneimine
Lipases
Lipase
Encapsulation
Silicon Dioxide

β-Glucosyl Fluoride as Reverse Reaction Donor Substrate and Mechanistic Probe of Inverting Sugar Nucleotide-Dependent Glycosyltransferases

Lepak, A., Gutmann, A. & Nidetzky, B., 5 Oct 2018, In : ACS Catalysis. 8, 10, p. 9148-9153 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Flavonoids
Glycosyltransferases
Diphosphates
Uridine
Nucleotides
2017

An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

Gutmann, A., Schiller, M., Gruber-Khadjawi, M. & Nidetzky, B., 5 Sep 2017, In : Organic & biomolecular chemistry. 15, p. 7917 7924 p.

Research output: Contribution to journalArticleResearchpeer-review

A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

Valikhani, D., Bolivar, J. M., Viefhues, M., McIlroy, D. N., Vrouwe, E. X. & Nidetzky, B., 11 Oct 2017, In : ACS Applied Materials & Interfaces. 9, 40, p. 34641-34649 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Enzyme immobilization
Microfluidics
Silicon Dioxide
Enzymes
Silica

Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose

Kulmer, S. T., Gutmann, A., Lemmerer, M. & Nidetzky, B., 2017, In : Advanced Synthesis & Catalysis. 359, p. 292-301

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Sugar (sucrose)
Nucleotides
Nucleosides
Glucose
Diphosphates

Debottlenecking enzymatic production of fucosylated HMOs with prokaryotic a1,2-fucosyltransferases

Petschacher, B., Gierlinger, K., Brunner, L., Kuballa, J. & Nidetzky, B., 23 Apr 2017.

Research output: Contribution to conferencePosterResearchpeer-review

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

Research output: Contribution to journalArticleResearchpeer-review

Uridine Diphosphate Glucose
Glycosyltransferases
Uridine Diphosphate
Recycling
Biological Products

Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation

Leis, D., Lauß, B., Macher-Ambrosch, R., Andreas, P., Nidetzky, B. & Kratzer, R., 2017, In : Journal of Biotechnology. 257, p. 110-117

Research output: Contribution to journalArticleResearchpeer-review

Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie / International Edition . 56, p. 2503 –2507

Research output: Contribution to journalArticleResearchpeer-review

Xylose
Uridine Diphosphate
Isotopes
Enzymes
Sugars

Multivalency Effects on the Immobilization of Sucrose Phosphorylase in Flow Microchannels and Their Use in the Development of a High-Performance Biocatalytic Microreactor

Valikhani, D., Bolivar, J. M., Pfeiffer, M. & Nidetzky, B., 9 Jan 2017, In : ChemCatChem. 9, 1, p. 161-166 6 p.

Research output: Contribution to journalArticleResearchpeer-review

sucrose phosphorylase
sucrose
Sugar (sucrose)
microchannels
Microchannels

Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

Bolivar, J. M., Luley-Goedl, C., Leitner, E., Sawangwan, T. & Nidetzky, B., 10 Sep 2017, In : Journal of Biotechnology. 257, p. 131-138 8 p.

Research output: Contribution to journalArticleResearchpeer-review

sucrose phosphorylase
Sugar (sucrose)
Glycerol
Catalyst supports
Enzymes

Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie. 129, p. 2544 –2548

Research output: Contribution to journalArticleResearchpeer-review

Hypergravity
Xylose
Genetic Engineering
Yeast
Fermentation

Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

Diricks, M., Gutmann, A., Debacker, S., Dewitte, G., Nidetzky, B. & Desmet, T., 2017, In : Protein engineering, design and selection. 30, 3, p. 141-148

Research output: Contribution to journalArticleResearchpeer-review

Uridine Diphosphate
Sugar (sucrose)
Nucleotides
Administrative data processing
Diphosphates

Single-molecule study of oxidative enzymaticdeconstruction of cellulose

Eibinger, M., Sattelkow, J., Ganner, T., Plank, H. & Nidetzky, B., 2017, In : Nature Communications . p. 8:894 7 p.

Research output: Contribution to journalArticleResearch

Tailor-made resealable micro(bio)reactors providing easy integration of in situ sensors

Viefhues, M., Sun, S., Valikhani, D., Nidetzky, B., Vrouwe, E. X., Mayr, T. & Bolivar, J. M., 10 May 2017, In : Journal of Micromechanics and Microengineering. 27, 6, 065012.

Research output: Contribution to journalArticleResearchpeer-review

Oxygen sensors
Immobilized Enzymes
Glucose Oxidase
Glucose oxidase
Optical sensors

Toward “homolactic” fermentation of glucose and xylose by engineered Saccharomyces cerevisiae harboring a kinetically efficient l-lactate dehydrogenase within pdc1-pdc5 deletion background

Novy, V., Brunner, B., Müller, G. & Nidetzky, B., 1 Jan 2017, In : Biotechnology and Bioengineering. 114, 1, p. 163-171 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Xylose
L-Lactate Dehydrogenase
Yeast
Fermentation
Glucose

Walking a Fine Line with Sucrose Phosphorylase: Efficient Single-Step Biocatalytic Production of l-Ascorbic Acid 2-Glucoside from Sucrose

Gudiminchi, R. K. & Nidetzky, B., 18 Jul 2017, In : ChemBioChem. 18, 14, p. 1387-1390 4 p.

Research output: Contribution to journalArticleResearchpeer-review

sucrose phosphorylase
Ascorbic Acid
Sucrose
Cosmetics
Protonation
2016

Advanced characterization of immobilized enzymes as heterogeneous biocatalysts

Bolivar, J. M., Eisl, I. & Nidetzky, B., 1 Jan 2016, In : Catalysis Today. 259, p. 66-80 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Immobilized Enzymes
Biocatalysts
Enzymes
Catalyst supports
Proteins
Fucosyltransferases
Oligosaccharides
Human Milk
galactoside 3-fucosyltransferase
Guanosine Diphosphate Fucose

Cellular automata modeling depicts degradation of cellulosic material by a cellulase system with single-molecule resolution

Eibinger, M., Zahel, T., Ganner, T., Plank, H. & Nidetzky, B., 8 Mar 2016, In : Biotechnology for Biofuels . 9, 56

Research output: Contribution to journalArticleResearchpeer-review

CREATING A WATER-SOLUBLE RESVERATROL-BASED ANTIOXIDANT THROUGH SITE-SELECTIVE ENZYMATIC GLUCOSYLATION

Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 14 Jul 2016.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Development of a fully integrated falling film microreactor for gas-liquid-solid biotransformation with surface immobilized O2-dependent enzyme

Bolivar Bolivar, J. M., Krämer, C. E. M., Ungerböck, B., Mayr, T. & Nidetzky, B., 2016, In : Biotechnology and Bioengineering.

Research output: Contribution to journalArticleResearchpeer-review

Biotransformation
Enzymes
Gases
Immobilization
Oxidoreductases

Direct-Write Fabrication of Cellulose Nano-Structures via Focused Electron Beam Induced Nanosynthesis

Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2 Sep 2016, In : Scientific reports. p. 1-11 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Downstream Processing of Nucleoside-Diphospho-Sugars from Sucrose Synthase Reaction Mixtures at Decreased Solvent Consumption

Lemmerer, M., Schmoelzer, K., Gutmann, A. & Nidetzky, B., 2016, In : Advanced Synthesis & Catalysis. 358, p. 3113-3122

Research output: Contribution to journalArticleResearchpeer-review

Sugar (sucrose)
Nucleosides
Sugars
Chromatography
Anions

Exploiting the biocatalytic potential of glycosyltransferases through in situ sugar donor recycling

Gutmann, A., Bungaruang, L., Lepak, A. & Nidetzky, B., 14 Nov 2016.

Research output: Contribution to conferencePosterResearch

Glycosyltransferases
Diphosphates
Uridine
Sugars
Recycling

Fabrication of Cellulose Structures via Focused Electron Beam Induced Conversion: Approaching the Nanoscale

Ganner, T., Sattelkow, J., Rumpf, B., Eibinger, M., Reishofer, D., Winkler, R., Nidetzky, B., Spirk, S. & Plank, H., 2016.

Research output: Contribution to conference(Old data) Lecture or PresentationResearch

Functional characterization of thenative swollenin from Trichoderma reesei:study of its possible role as C1 factor ofenzymatic lignocellulose conversion

Eibinger, M., Sigl, K., Sattelkow, J., Ganner, T., Ramoni, J., Seiboth, B., Plank, H. & Nidetzky, B., 2016, In : Biotechnology for Biofuels . p. 9:178

Research output: Contribution to journalArticleResearchpeer-review

Improving the Water Solubility of Polyphenols through Site-Selective Enzymatic Glucosylation

Lepak, A., Gutmann, A., Kulmer, S. T. & Nidetzky, B., 12 Jun 2016.

Research output: Contribution to conferencePosterResearch

Kinetic analysis and probing with substrate analogues of the reaction pathway of the nitrile reductase QueF from Escherichia coli

Jung, J., Czabany, T., Wilding, B., Klempier, N. & Nidetzky, B., 2016, In : The Journal of Biological Chemistry. 291, 49, p. 25411-25426 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Nitriles
Escherichia coli
Oxidoreductases
Kinetics
Substrates

Let the substrate flow, not the enzyme: Practical immobilization of d-amino acid oxidase in a glass microreactor for effective biocatalytic conversions

Bolivar Bolivar, J. M., Tribulato, M. A., Petrasek, Z. & Nidetzky, B., 1 Nov 2016, In : Biotechnology and Bioengineering. 113, 11, p. 2342-2349 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Enzyme immobilization
Immobilization
Glass
Amino acids
Oxidoreductases

Prokaryotic fucosyltransferases for biotechnological production of fucosylated human milk oligosaccharides

Petschacher, B., Gierlinger, K., Brunner, L., Rinner, G., Kuballa, J. & Nidetzky, B., 14 Nov 2016.

Research output: Contribution to conferencePosterResearch

Prokaryotic fucosyltransferases for biotechnological production of fucosylated human milk oligosaccharides

Petschacher, B., Gierlinger, K., Brunner, L., Rinner, G., Kuballa, J. & Nidetzky, B., 13 Sep 2016.

Research output: Contribution to conferencePosterResearchpeer-review