Research Output 1981 2019

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2019

A functionally-distinct carboxylic acid reductase (PcCAR4) unearthed from a repertoire of type IV CARs in the white-rot fungus Pycnoporus cinnabarinus

Ling, J. G., Mansor, M. H., Murad, M. A., Khalid, R. M., Quay, D. H-X., Winkler, M. & Bakar, F. D. A., 2019, In : Journal of Biotechnology. 2020, 307, p. 55-62

Research output: Contribution to journalArticleResearchpeer-review

Pycnoporus
Fungi
Carboxylic acids
Genes
Enzymes

Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol

Horvat, M., Fritsche, S., Kourist, R. & Winkler, M., 19 Mar 2019, In : ChemCatChem. p. 4171-4181

Research output: Contribution to journalArticleResearchpeer-review

carboxylates
Oxidoreductases
preparation
cells
aldehydes

Co-factor demand and regeneration in the enzymatic one-step reduction of carboxylates to aldehydes in cell-free systems

Strohmeier, G., Schwarz, A., Andexer, J. N. & Winkler, M., 28 Oct 2019, (Accepted/In press) In : Journal of Biotechnology. 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Cell-Free System
Aldehydes
Regeneration
Adenosine Triphosphate
Sinorhizobium meliloti

Combining Photo-Organo Redox- and Enzyme Catalysis Facilitates Asymmetric C-H Bond Functionalization

Zhang, W., Fernández-Fueyo, E., Hollmann, F., Leemans Martin, L., Pesic, M., Wardenga, R., Höhne, M. & Schmidt, S., 10 Jan 2019, In : European journal of organic chemistry. 2019, 1, p. 80-84 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Rinnofner, C., Kerschbaumer, B., Weber, H., Glieder, A. & Winkler, M., 2019, In : Biocatalysis and Agricultural Biotechnology. 17, p. 525-528 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
ibuprofen
Hydroxylation
Pichia pastoris
Biocatalysts
Pichia

Discovery of carboxylic acid reductase (CAR) from Thermothelomyces thermophilus and its evaluation for vanillin synthesis

Horvat, M., Fiume, G., Fritsche, S. & Winkler, M., 12 Aug 2019, In : Journal of Biotechnology. 304, p. 44-51

Research output: Contribution to journalArticleResearchpeer-review

Carboxylic acids
Substrates
Vanillic Acid
Eugenol
Acids

Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH

Strohmeier, G., Eiteljörg, I., Schwarz, A. & Winkler, M., 26 Apr 2019, In : Chemistry - A European Journal. 25, 24, p. 6119-6123 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Adenosinetriphosphate
Aldehydes
NADP
Carboxylic Acids
Adenosine Triphosphate

Exploring the biocatalytic potential of a cytochrome P450 from Thermothelomyces thermophila

Fürst, M. J. L. J., Kerschbaumer, B., Rinnofner, C., Migglautsch, A. K., Winkler, M. & Fraaije, M. W., 2019, (Accepted/In press) In : Advanced Synthesis & Catalysis. p. 2487 2496 p.

Research output: Contribution to journalArticleResearchpeer-review

Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator

Assil Companioni, L., Schmidt, S., Heidinger, P., Schwab, H. & Kourist, R., 19 Mar 2019, In : ChemSusChem. 12, 11, p. 2361-2365 5 p.

Research output: Contribution to journalArticleResearchpeer-review

biotransformation
Hydrogen
regeneration
hydrogen
Hydrogenase

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Fischer, J. E., Hatzl, A-M., Weninger, A., Schmid, C. & Glieder, A., 2019, In : Journal of visualized experiments . 2019, 143, e58589.

Research output: Contribution to journalArticleResearchpeer-review

Pichia
Methanol
Technology
Bioreactors
Carbon

Random Mutagenesis-Driven Improvement of Carboxylate Reductase Activity Using an Amino Benzamidoxime-Mediated High-Throughput Assay

Schwendenwein, D., Ressmann, A. K., Dörr, M., Höhne, M., Mihovilovic, M. D., Bornscheuer, U. T., Rudroff, F. & Winkler, M., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2544-2549 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Mutagenesis
Carboxylic acids
Assays
Oxidoreductases
Throughput

Substrate-Independent High-Throughput Assay for the Quantification of Aldehydes

Ressmann, A. K., Schwendenwein, D., Leonhartsberger, S., Mihovilovic, M. D., Bornscheuer, U. T., Winkler, M. & Rudroff, F., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2538-2543 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Aldehydes
Carboxylic acids
Assays
Throughput
Substrates
2018

Aliphatic hydroxylation and epoxidation of capsaicin by Cytochrome P450 CYP505X

Migglautsch, A. K., Willim, M., Schweda, B., Glieder, A., Breinbauer, R. & Winkler, M., 31 Aug 2018, In : Tetrahedron. 74, p. 6199-6204

Research output: Contribution to journalArticleResearchpeer-review

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

Research output: Contribution to journalArticleResearchpeer-review

decarboxylation
Carboxy-Lyases
fatty acids
Hydrocarbons
Fatty acids

Biocatalytic oxidation reactions - a Chemist's perspective

Dong, J., Fernández-Fueyo, E., Hollmann, F., Paul, C., Pasic, M., Schmidt, S., Wang, Y., Younes, S. & Zhang, W., Jul 2018, In : Angewandte Chemie / International Edition . 57, 30, p. 9238-9261

Research output: Contribution to journalArticleResearchpeer-review

Oxidation
Oxidoreductases
Enzymes
Halogenation
Redox reactions

Cloning and characterization of a new delta-specific L-leucine dioxygenase from Anabaena variabilis

Correia Cordeiro, R. S., Enoki, J., Busch, F., Mügge, C. & Kourist, R., 20 Oct 2018, In : Journal of Biotechnology. 284, p. 68-74 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Anabaena variabilis
Dioxygenases
Cloning
Leucine
Amino acids

Construction of a cellulose-metabolizing Komagataella phaffii (Pichia pastoris) by co-expressing glucanases and β-glucosidase

Kickenweiz, T., Glieder, A. & Wu, J. C., 2018, In : Applied Microbiology and Biotechnology. 102, 3, p. 1297-1306

Research output: Contribution to journalArticleResearchpeer-review

Glucosidases
Pichia
Cellulose
Cellobiose
Cellulases

Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

Liu, Y., Gong, R., Liu, X., Zhang, P., Zhang, Q., Cai, Y-S., Deng, Z., Winkler, M., Wu, J. & Chen, W., 2018, In : Microbial cell factories . 17, 10 p., 131.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Identification of key residues for enzymatic carboxylate reduction

Stolterfoht, H., Steinkellner, G., Schwendenwein, D., Pavkov-Keller, T., Gruber, K. & Winkler, M., 12 Feb 2018, In : Frontiers in Microbiology . 9, 250.

Research output: Contribution to journalArticleResearchpeer-review

Modification of Membrane Lipid Compositions in single-celled organisms–from Basics to Applications

Pichler, H. & Emmerstorfer-Augustin, A., 13 Sep 2018, In : Methods. 147, p. 50 - 65 16 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

On the current role of hydratases in biocatalysis

Engleder, M. & Pichler, H., 15 Jul 2018, In : Applied Microbiology and Biotechnology. 102, p. 5841-5858

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Parallelized biocatalytic scanning probe lithography for the additive fabrication of conjugated polymer structures

Hosford, J., Valles, M., Krainer, F. W., Glieder, A. & Wong, L. S., 2018, In : Nanoscale. 10, 15, p. 7185-7193 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Conjugated polymers
Lithography
Scanning
Fabrication
Polymers

Perdeuteration of cholesterol for neutron scattering applicatioins using recombinant Pichia pastoris

Mouline, M., Strohmeier, G., Hirz, M., Thompson, K., Rennie, A., Campbell, R. A., Pichler, H., Maric, S., Forsyth, T. & Haertlein, M., 5 Feb 2018, In : Chemistry and physics of lipids. 212, p. 80-87

Research output: Contribution to journalArticleResearchpeer-review

Phosphorylation by the stress-activated MAPK Slt2 down-regulates the yeast TOR complex 2

Leskoske, K. L., Roelants, F. M., Emmerstorfer-Augustin, A., Augustin, C. M., Si, E. P., Hill, J. M. & Thorner, J., 1 Dec 2018, In : Genes and Development. 32, 23-24, p. 1576-1590 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Mitogen-Activated Protein Kinases
Down-Regulation
Yeasts
Phosphorylation
Alleles

Phylogenomics uncovers early hybridization and adaptive loci shaping the radiation of Lake Tanganyika cichlid fishes

Irisarri, I., Singh, P., Koblmüller, S., Torres-Dowdall, J., Henning, F., Franchini, P., Fischer, C., Lemmon, A. R., Lemmon, E. M., Thallinger, G. G., Sturmbauer, C. & Meyer, A., 8 Aug 2018, In : Nature Communications. 9, 1, 3159.

Research output: Contribution to journalArticleResearchpeer-review

Cichlids
Tanzania
fishes
loci
Lakes

Production of Hydroxynitrile Lyase from Davallia tyermannii (DtHNL) in Komagataella phaffii and Its Immobilization as a CLEA to Generate a Robust Biocatalyst

Lanfranchi, E., Grill, B., Raghoebar, Z., Van Pelt, S., Sheldon, R. A., Steiner, K., Glieder, A. & Winkler, M., 2018, In : ChemBioChem. 19, 4, p. 312-316 10.1002/cbic.201700419.

Research output: Contribution to journalArticleResearchpeer-review

mandelonitrile lyase
Immobilization
Enzymes
Ferns
Agrochemicals

Raman Microspectroscopic Evidence for the Metabolism of a Tyrosine Kinase Inhibitor, Neratinib, in Cancer Cells

Aljakouch, K., Lechtonen, T., Yosef, H. K., Hammoud, M. K., Alsaidi, W., Kötting, C., Mügge, C., Kourist, R., El-Mashtoly, S. F. & Gerwert, K., 11 Jun 2018, In : Angewandte Chemie / International Edition . 57, 24, p. 7250-7254 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Metabolites
Metabolism
Protein-Tyrosine Kinases
Microscopic examination
Cells

Recombinant expression, purification and biochemical characterization of kievitone hydratase from Nectria haematococca

Engleder, M., Horvat, M., Emmerstorfer-Augustin, A., Wriessnegger, T., Gabriel, S., Strohmeier, G., Weber, H., Müller, M., Kaluzna, I., Mink, D., Schürmann, M. & Pichler, H., 8 Feb 2018, In : PLoS ONE.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

The Extreme Structural Plasticity in the CYP153 Subfamily of P450s Directs Development of Designer Hydroxylases

Fiorentini, F., Hatzl, A-M., Schmidt, S., Savino, S., Glieder, A. & Mattevi, A., 2018, In : Biochemistry. 57, 48, p. 6701-6714

Research output: Contribution to journalArticleResearchpeer-review

Mixed Function Oxygenases
Plasticity
Hydroxylation
lauric acid
Substrates

The Impact of Deuteration on Natural and Synthetic Lipids: a Neutron Diffraction Study

Luchini, A., Delhom, R., Demé, B., Laux, V., Mouline, M., Haertlein, M., Pichler, H., Strohmeier, G., Wacklin, H. & Fragneto, G., 1 Aug 2018, In : Colloids and Surfaces / B. p. 126 - 133

Research output: Contribution to journalArticleResearchpeer-review

Whole-cell (+)-ambrein production in the yeast Pichia pastoris

Moser, S., Strohmeier, G., Leitner, E., Plocek, T. J., Vanhessche, K. & Pichler, H., Dec 2018, In : Metabolic Engineering Communications. 7, 9 p., e00077.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
2017

An alternative approach towards poly-epsilon-caprolactone through a chemoenzymatic synthesis: combined hydrogenation, bio-oxidations and polymerization without the isolation of intermediates

Wedde, S., Rommelmann, P., Scherkus, C., Schmidt, S., Bornscheuer, U. T., Liese, A. & Gröger, H., 2017, In : Green chemistry. 19, 5

Research output: Contribution to journalArticleResearchpeer-review

Cloning and upscale production of monoamine oxidase N (MAO-N D5) by Pichia pastoris

Markošová, K., Camattari, A., Rosenberg, M., Glieder, A., Turner, N. J. & Rebroš, M., 2017, In : Biotechnology Letters. https://doi.org/10.1007/s10529-017-2450-y.

Research output: Contribution to journalArticleResearchpeer-review

Pichia
Monoamine Oxidase
Organism Cloning
Enzymes
Clone Cells

Crystal Structure and Catalytic Mechanism of CouO, a Versatile C-Methyltransferase from Streptomyces rishiriensis

Pavkov-Keller, T., Steiner, K., Faber, M., Tengg, M., Schwab, H., Gruber-Khadjawi, M. & Gruber, K., 2017, In : PLoS ONE. 12, p. 1-14 e0171056.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Durch Trennen verbinden

Schmidt, S., Gröger, H. & Kourist, R., Oct 2017, In : Nachrichten aus der Chemie. 65, 10, p. 985-988 4 p.

Research output: Contribution to journalArticleResearch

Enzymatic Oxyfunctionalization Driven by Photosynthetic Water-Splitting in the Cyanobacterium Synechocystis sp. PCC 6803

Böhmer, S., Köninger, K., Gomez-Baraibar, A., Bojarra, S., Mügge, C., Schmidt, S., Nowaczyk, M. & Kourist, R., Aug 2017, In : Catalysts. 7, 8, 8 p., 240.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
water splitting
regeneration
Fueling
Oxygenation
Niacinamide

Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Lanfranchi, E., Pavkov-Keller, T., Koehler, E-M., Diepold, M., Steiner, K., Darnhofer, B., Hartler, J., Van Den Bergh, T., Joosten, H-J., Gruber-Khadjawi, M., Thallinger, G. G., Birner-Gruenberger, R., Gruber, K., Winkler, M. & Glieder, A., 10 May 2017, In : Scientific reports. 7, 46738.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
mandelonitrile lyase
Enzymes
Biocatalysis
Ferns
Crystallography

Erratum: Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Lanfranchi, E., Pavkov-Keller, T., Koehler, E-M., Diepold, M., Steiner, K., Darnhofer, B., Hartler, J., Bergh, T. V. D., Joosten, H-J., Gruber-Khadjawi, M., Thallinger, G. G., Birner-Gruenberger, R., Gruber, K., Winkler, M. & Glieder, A., 26 May 2017, In : Scientific reports. 7, 46834.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
mandelonitrile lyase
Enzymes

Expanding the CRISPR/Cas9 toolkit for Pichia pastoris with efficient donor integration and alternative resistance markers

Weninger, A., Fischer, J., Raschmanová, H., Kniely, C., Vogl, T. & Glieder, A., 1 Nov 2017, In : Journal of Cellular Biochemistry.

Research output: Contribution to journalArticleResearchpeer-review

Clustered Regularly Interspaced Short Palindromic Repeats
Pichia
Homologous Recombination
Guide RNA
Genes

Four distinct types of E.C. 1.2.1.30 enzymes can catalyze the reduction of carboxylic acids to aldehydes

Stolterfoht, H., Schwendenwein, D., Sensen, C. W., Rudroff, F. & Winkler, M., 2017, In : Journal of Biotechnology. 257, p. 222-232

Research output: Contribution to journalArticleResearchpeer-review

Human germ/stem cell-specific gene TEX19 influences cancer cell proliferation and cancer prognosis

Planells-Palop, V., Hazazi, A., Feichtinger, J., Jezkova, J., Thallinger, G., Alsiwiehri, N. O., Almutairi, M., Parry, L., Wakeman, J. A. & McFarlane, R. J., 26 Apr 2017, In : Molecular Cancer. 16, 1, 84.

Research output: Contribution to journalArticleResearchpeer-review

Germ Cells
Stem Cells
Cell Proliferation
Genes
Neoplasms

IN VIVO SYNTHESIS OF POLYHYDROXYLATED COMPOUNDS FROM A ‘HIDDEN RESERVOIR’ OF TOXIC ALDEHYDE SPECIES

Bayer, T., Wiesinger, T., Milker, S., Winkler, M., Mihovilovic, M. D. & Rudroff, F., 2017, In : ChemCatChem. 9, p. 2919-2923

Research output: Contribution to journalArticleResearchpeer-review

Localization of Cholesterol within Supported Lipid Bilayers Made of a Natural Extract of Tailor-Deuterated Phosphatidylcholine

Waldie, S., Lind, T. K., Browning, K., Mouline, M., Haertlein, M., Forsyth, T., Luchini, A., Strohmeier, G., Pichler, H., Maric, S. & Cárdenas, M., 27 Dec 2017, In : Langmuir. 34, 1, p. 472-479 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipid bilayers
Cholesterol
cholesterol
Lipid Bilayers
Phosphatidylcholines

NR4A3 Suppresses Lymphomagenesis through Induction of Proapoptotic Genes

Deutsch, A. J. A., Rinner, B., Pichler, M., Prochazka, K., Pansy, K., Bischof, M., Fechter, K., Hatzl, S., Feichtinger, J., Wenzl, K., Frisch, M-T., Stiegelbauer, V., Prokesch, A., Krogsdam, A., Sill, H., Thallinger, G. G., Greinix, H. T., Wang, C., Beham-Schmid, C. & Neumeister, P., 1 May 2017, In : Cancer Research. 77, 9, p. 2375-2386

Research output: Contribution to journalArticleResearchpeer-review

Lymphoma
Genes
Neoplasms
Nuclear Receptor Subfamily 4, Group A, Member 1
Puma

Overcoming the incompatibility challenge in chemo-enzymatic and multi-catalytic cascade reactions.

Schmidt, S., Castiglione, K. & Kourist, R., 6 Sep 2017, In : Chemistry - A European Journal.

Research output: Contribution to journalArticleResearchpeer-review

Photobiocatalytic alcohol oxidation using LED light sources

Rauch, M., Schmidt, S., Arends, I. W. C. E., Oppelt, K., Kara, S. & Hollmann, F., 2017, In : Green chemistry. 19, 2

Research output: Contribution to journalArticleResearchpeer-review

Pichia pastoris Alcohol Oxidase 1 (AOX1) Core Promoter Engineering by High Resolution Systematic Mutagenesis

Portela, R. M. C., Vogl, T., Ebner, K., Oliveira, R. & Glieder, A., 10 Nov 2017, In : Biotechnology Journal. 10.1002/biot.201700340.

Research output: Contribution to journalArticleResearchpeer-review

alcohol oxidase
Pichia
5' Untranslated Regions
Mutagenesis
Mutation

Process Development through Solvent Engineering in the Biocatalytic Synthesis of the Heterocyclic Bulk Chemical ε-Caprolactone

Reimer, A., Wedde, S., Staudt, S., Schmidt, S., Höffer, D., Hummel, W., Kragl, U., Bornscheuer, U. T. & Gröger, H., 2017, In : Journal of heterocyclic chemistry. 54, 1, p. 391-396 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Synthetic Core Promoters as Universal Parts for Fine-Tuning Expression in Different Yeast Species

Portela, R. M. C., Vogl, T., Kniely, C., Fischer, J. E., Oliveira, R. & Glieder, A., 17 Mar 2017, In : ACS Synthetic Biology . 6, 3, p. 471-484 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Synthetic Biology
Gene expression
Yeast
Tuning
Yeasts

Towards environmentally acceptable synthesis of chiral alpha-hydroxy ketones via oxidase-lyase cascades

Schmidt, S., Pedroso de Almeida, T., Rother, D. & Hollmann, F., 2017, In : Green chemistry. 19, 5

Research output: Contribution to journalArticleResearchpeer-review