Research Output 1981 2019

2019

3-Hydroxytyrosol: A whole cell mediated Bioreduction using a type IV Carboxylate Reductase

Horvat, M. & Winkler, M., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

A functionally-distinct carboxylic acid reductase (PcCAR4) unearthed from a repertoire of type IV CARs in the white-rot fungus Pycnoporus cinnabarinus

Ling, J. G., Mansor, M. H., Murad, M. A., Khalid, R. M., Quay, D. H-X., Winkler, M. & Bakar, F. D. A., 2019, In : Journal of Biotechnology. 2020, 307, p. 55-62

Research output: Contribution to journalArticleResearchpeer-review

Pycnoporus
Fungi
Carboxylic acids
Genes
Enzymes

A Multi-Enzyme System for the In vitro Reduction of Carboxylic Acids to Aldehydes

Strohmeier, G., Schwarz, A., Eiteljörg, I., Kohrt, J., Howard, R. & Winkler, M., Jul 2019.

Research output: Contribution to conferencePosterResearch

Capsaicin Metabolite Synthesis by Fungal Cytochrome P450

Willim, M., Bachler, T., Schweda, B., Migglautsch, A. K., Glieder, A., Breinbauer, R. & Winkler, M., 14 Feb 2019.

Research output: Contribution to conferencePosterResearch

Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol

Horvat, M., Fritsche, S., Kourist, R. & Winkler, M., 19 Mar 2019, In : ChemCatChem. p. 4171-4181

Research output: Contribution to journalArticleResearchpeer-review

carboxylates
Oxidoreductases
preparation
cells
aldehydes

Co-factor demand and regeneration in the enzymatic one-step reduction of carboxylates to aldehydes in cell-free systems

Strohmeier, G., Schwarz, A., Andexer, J. N. & Winkler, M., 28 Oct 2019, (Accepted/In press) In : Journal of Biotechnology. 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Cell-Free System
Aldehydes
Regeneration
Adenosine Triphosphate
Sinorhizobium meliloti

Combined Enzymatic Decarboxylation and Pd-catalyzed Heck Coupling in Continuous Flow

Grabner, B., Schweiger, A. K., Gavric, K., Kourist, R. & Gruber-Wölfler, H., Sep 2019.

Research output: Contribution to conferencePosterResearchpeer-review

Combining Photo-Organo Redox- and Enzyme Catalysis Facilitates Asymmetric C-H Bond Functionalization

Zhang, W., Fernández-Fueyo, E., Hollmann, F., Leemans Martin, L., Pesic, M., Wardenga, R., Höhne, M. & Schmidt, S., 10 Jan 2019, In : European journal of organic chemistry. 2019, 1, p. 80-84 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Rinnofner, C., Kerschbaumer, B., Weber, H., Glieder, A. & Winkler, M., 2019, In : Biocatalysis and Agricultural Biotechnology. 17, p. 525-528 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
ibuprofen
Hydroxylation
Pichia pastoris
Biocatalysts
Pichia

Discovery of carboxylic acid reductase (CAR) from Thermothelomyces thermophilus and its evaluation for vanillin synthesis

Horvat, M., Fiume, G., Fritsche, S. & Winkler, M., 12 Aug 2019, In : Journal of Biotechnology. 304, p. 44-51

Research output: Contribution to journalArticleResearchpeer-review

Carboxylic acids
Substrates
Vanillic Acid
Eugenol
Acids

Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH

Strohmeier, G., Eiteljörg, I., Schwarz, A. & Winkler, M., 26 Apr 2019, In : Chemistry - A European Journal. 25, 24, p. 6119-6123 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Adenosinetriphosphate
Aldehydes
NADP
Carboxylic Acids
Adenosine Triphosphate

Enzyme-catalyzed synthesis of amides from nitriles

Glänzer, M., Grill, B., Schwab, H., Pogorevcnik, U., Reicher, K., Donsbach, K., Pienaar, D. & Winkler, M., Sep 2019.

Research output: Contribution to conferencePosterResearch

Expanding the potential of phenolic acid decarboxylase from Bacillus subtilis by use of non-conventional solvents

Schweiger, A. K., Ríos-Lombardía, N., Winkler, C. K., Schmidt, S., Morís, F., Kroutil, W., González-Sabín, J. & Kourist, R., Jul 2019.

Research output: Contribution to conferencePosterResearch

Exploring the biocatalytic potential of a cytochrome P450 from Thermothelomyces thermophila

Fürst, M. J. L. J., Kerschbaumer, B., Rinnofner, C., Migglautsch, A. K., Winkler, M. & Fraaije, M. W., 2019, (Accepted/In press) In : Advanced Synthesis & Catalysis. p. 2487 2496 p.

Research output: Contribution to journalArticleResearchpeer-review

From One-pot to One-flow: A chemo-enzymatic tandem reaction in continuous flow

Grabner, B., Schweiger, A. K., Gavric, K., Kourist, R. & Gruber-Wölfler, H., Sep 2019.

Research output: Contribution to conferencePosterResearch

Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator

Assil Companioni, L., Schmidt, S., Heidinger, P., Schwab, H. & Kourist, R., 19 Mar 2019, In : ChemSusChem. 12, 11, p. 2361-2365 5 p.

Research output: Contribution to journalArticleResearchpeer-review

biotransformation
Hydrogen
regeneration
hydrogen
Hydrogenase

Membrane protein production in yeast: Modification of yeast membranes for human membrane protein production

Emmerstorfer-Augustin, A., Wriessnegger, T., Hirz, M., Zellnig, G. & Pichler, H., 9 Feb 2019, Recombinant Protein Production in Yeast: Methods and Protocols. Springer, (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies

Fischer, J. E., Hatzl, A-M., Weninger, A., Schmid, C. & Glieder, A., 2019, In : Journal of visualized experiments . 2019, 143, e58589.

Research output: Contribution to journalArticleResearchpeer-review

Pichia
Methanol
Technology
Bioreactors
Carbon

Optimizing the heterologous expression of the carboxylic acid reductase from Nocardia otitidiscavium for the reduction of aromatic acids

Weber, D., Patsch, D., Winkler, M., Turner, N. & Rother, D., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

Photo-Biocatalysis: Biotransformations in the Presence of Light

Schmermund, L., Jurkaš, V., Özgen, F. F., Barone, G. D., Büchsenschütz, H. C., Winkler, C. K., Schmidt, S., Kourist, R. & Kroutil, W., 2019, In : ACS Catalysis. 9, 5, p. 4115-4144 30 p.

Research output: Contribution to journalReview articleResearchpeer-review

Chemical reactions
Enzymes
Biotransformation
Biocatalysis
Biocatalysts

Random Mutagenesis-Driven Improvement of Carboxylate Reductase Activity Using an Amino Benzamidoxime-Mediated High-Throughput Assay

Schwendenwein, D., Ressmann, A. K., Dörr, M., Höhne, M., Mihovilovic, M. D., Bornscheuer, U. T., Rudroff, F. & Winkler, M., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2544-2549 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Mutagenesis
Carboxylic acids
Assays
Oxidoreductases
Throughput

Random mutagenesis of carboxylate reductase enabled by a substrate independent HT-assay

Rudroff, F., Winkler, M., Schwendenwein, D., Ressmann, A. K., Mihovilovic, M. D., Bornscheuer, U. T. & Dörr, M., Jul 2019.

Research output: Contribution to conferencePosterResearch

Recombinant lipoxygenases (LOX) and hydroperoxide lyases (HPL) for the synthesis of green leaf volatiles

Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., 8 Oct 2019, (Accepted/In press) In : Journal of agricultural and food chemistry. 59 p.

Research output: Contribution to journalReview articleResearchpeer-review

Lipoxygenases
lyases
hydroperoxides
lipoxygenase
synthesis

Recombinant lipoxygenases and hydroperoxide lyases for the synthesis of green leaf volatiles

Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., 2019, (Accepted/In press) In : Journal of agricultural and food chemistry.

Research output: Contribution to journalReview articleResearchpeer-review

Structure elucidation of the carboxylate reductase from Neurospora crassa (NcCAR)

Daniel, B., Pavkov-Keller, T., Leithold, M., Gruber, K., Stolterfoht, H. & Winkler, M., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

Substrate-Independent High-Throughput Assay for the Quantification of Aldehydes

Ressmann, A. K., Schwendenwein, D., Leonhartsberger, S., Mihovilovic, M. D., Bornscheuer, U. T., Winkler, M. & Rudroff, F., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2538-2543 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Aldehydes
Carboxylic acids
Assays
Throughput
Substrates

Towards a new panel of nitrile hydratases

Grill, B., Schwab, H., Glänzer, M., Steinkellner, G., Strohmeier, G., Pogorevcnik, U., Reicher, K., Gruber, K., Donsbach, K. & Winkler, M., 26 Jun 2019.

Research output: Contribution to conferencePosterResearch

Valorization of Fatty Acids by Pichia pastoris: A Fresh Approach for Green Leaf Volatile Synthesis

Hashem, C., Steinberger, S., Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., 12 Nov 2019.

Research output: Contribution to conferencePosterResearch

2018

A light-driven enzyme cascade reaction for the synthesis of lactones

Büchsenschütz, H. C., Schultes, F., Schmidt, S. & Kourist, R., 9 Apr 2018.

Research output: Contribution to conferencePosterResearch

A light-driven enzyme cascade reaction for the synthesis of lactones.

Büchsenschütz, H. C., Schultes, F., Schmidt, S. & Kourist, R., 26 Aug 2018.

Research output: Contribution to conferencePosterResearch

Aliphatic hydroxylation and epoxidation of capsaicin by Cytochrome P450 CYP505X

Migglautsch, A. K., Willim, M., Schweda, B., Glieder, A., Breinbauer, R. & Winkler, M., 31 Aug 2018, In : Tetrahedron. 74, p. 6199-6204

Research output: Contribution to journalArticleResearchpeer-review

Alternative Binding Mode impairs Enantioselectivity of Arylmalonate Decarboxylase

Schweiger, A. K., Enoki, J., Mügge, C. & Kourist, R., Aug 2018.

Research output: Contribution to conferencePosterResearch

A novel assay for the detection of carboxylic acid reductase (CAR) activity in whole cells

Schwendenwein, D., Ressmann, A. K., Mihovilovic, M. D., Bornscheuer, U. T., Dörr, M., Rudroff, F. & Winkler, M., 27 Aug 2018.

Research output: Contribution to conferencePosterResearch

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

Research output: Contribution to journalArticleResearchpeer-review

decarboxylation
Carboxy-Lyases
fatty acids
Hydrocarbons
Fatty acids

Biocatalytic oxidation reactions - a Chemist's perspective

Dong, J., Fernández-Fueyo, E., Hollmann, F., Paul, C., Pasic, M., Schmidt, S., Wang, Y., Younes, S. & Zhang, W., Jul 2018, In : Angewandte Chemie / International Edition . 57, 30, p. 9238-9261

Research output: Contribution to journalArticleResearchpeer-review

Oxidation
Oxidoreductases
Enzymes
Halogenation
Redox reactions

Carboxylic acid reductase enzymes (CARs)

Winkler, M., 2018, In : Current opinion in chemical biology. 43, p. 23-29

Research output: Contribution to journalReview articleResearchpeer-review

Cloning and characterization of a new delta-specific L-leucine dioxygenase from Anabaena variabilis

Correia Cordeiro, R. S., Enoki, J., Busch, F., Mügge, C. & Kourist, R., 20 Oct 2018, In : Journal of Biotechnology. 284, p. 68-74 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Anabaena variabilis
Dioxygenases
Cloning
Leucine
Amino acids

Combinatorial optimization strategies to increase Human P450 catalytic Activity in whole cell

Weninger, A., Alkofer, A., Schmid, C., Bitter, J., Volpini de Maestri, A., Pranjic, M., Winkler, M. & Glieder, A., Sep 2018.

Research output: Contribution to conferencePosterResearch

Construction of a cellulose-metabolizing Komagataella phaffii (Pichia pastoris) by co-expressing glucanases and β-glucosidase

Kickenweiz, T., Glieder, A. & Wu, J. C., 2018, In : Applied Microbiology and Biotechnology. 102, 3, p. 1297-1306

Research output: Contribution to journalArticleResearchpeer-review

Glucosidases
Pichia
Cellulose
Cellobiose
Cellulases

Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

Liu, Y., Gong, R., Liu, X., Zhang, P., Zhang, Q., Cai, Y-S., Deng, Z., Winkler, M., Wu, J. & Chen, W., 2018, In : Microbial cell factories . 17, 10 p., 131.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Enzymatic Decarboxylation as a Tool for the Enzymatic Defunctionalization of Hydrophobic Bio-based Organic Acids

Kourist, R., Schweiger, A. & Büchsenschütz, H., 2018, Lipid Modification by Enzymes and Engineered Microbes. Bornscheuer, U. T. (ed.). AOCS Press, p. 89-118 30 p.

Research output: Chapter in Book/Report/Conference proceedingChapterResearch

Erratum to: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization (Nature Communications, (2018), 9, 1, (3589), 10.1038/s41467-018-05915-w)

Vogl, T., Kickenweiz, T., Pitzer, J., Sturmberger, L., Weninger, A., Biggs, B. W., Köhler, E. M., Baumschlager, A., Fischer, J. E., Hyden, P., Wagner, M., Baumann, M., Borth, N., Geier, M., Ajikumar, P. K. & Glieder, A., 1 Dec 2018, In : Nature Communications. 9, 1, 4566.

Research output: Contribution to journalComment/debateResearchpeer-review

files
genes
Publications
Genes
communication

Fatty Alcohols: enzymatically from Free fatty Acids

Horvat, M., Zabielska, J., Kourist, R. & Winkler, M., 3 Jul 2018.

Research output: Contribution to conferencePosterResearch

Fragrance synthesis via a chemo- & multi enzyme cascade

Rudroff, F., Winkler, M., Entner, M., Schwendenwein, D., Ressmann, A. K. & Mihovilovic, M. D., Jul 2018.

Research output: Contribution to conferencePosterResearch

Humane Enzyme für die Organische Synthese

Winkler, M., Geier, M., Hanlon, S. P., Nidetzky, B. & Glieder, A., 2018, In : Angewandte Chemie. 130, p. 13592-13610

Research output: Contribution to journalReview articleResearchpeer-review

Human Enzymes for Organic Synthesis

Winkler, M., Geier, M., Hanlon, S. P., Nidetzky, B. & Glieder, A., 2018, In : Angewandte Chemie / International Edition . 57, 41, p. 13406-13423 18 p.

Research output: Contribution to journalReview articleResearchpeer-review

Enzymes
Pharmaceutical Preparations
Xenobiotics
Metabolites
Metabolism

Hybrid CARs (carboxylate reductases)

Stolterfoht, H., Gradischnig, D. & Winkler, M., 10 Oct 2018.

Research output: Contribution to conferencePosterResearch

Identification of key residues for enzymatic carboxylate reduction

Stolterfoht, H., Steinkellner, G., Schwendenwein, D., Pavkov-Keller, T., Gruber, K. & Winkler, M., 12 Feb 2018, In : Frontiers in Microbiology . 9, 250.

Research output: Contribution to journalArticleResearchpeer-review