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Research Output 1966 2020

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2020

Enzyme Immobilization in Wall-Coated Flow Microreactors

Valikhani, D., Bolivar, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 243-257 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Immobilization
Enzymes
sucrose phosphorylase
Silicon Dioxide
Glass

Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisan, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 319-333 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Immobilized Enzymes
Proteins
Enzymes
Penicillin Amidase
Proton-Motive Force
2019

Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry

Hengge, E., Steyskal, E-M., Bachler, R., Dennig, A., Nidetzky, B. & Würschum, R., 18 Nov 2019, In : Beilstein Journal of Nanotechnology . 10, p. 2275-2279 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
cysteine
Gold
Cysteine
Monolayers
Desorption

An integrated eco-system for pollution prevention and greening the production chain of small-scale rice-paper production: A case study from Vietnam

Hai, L. T., Thanh Tran, V., Vi, L. Q., Phuong Thao, N. T., Hieu, T. T., Braunegg, S., Schnitzer, H. & Braunegg, G., 9 Oct 2019, In : Journal of cleaner production. 28 p., 118785.

Research output: Contribution to journalArticleResearchpeer-review

Exhaust gases
Ashes
Farms
Wastewater
Pollution

A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase

Pfeiffer, M., Johansson, C., Krojer, T., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 5 Apr 2019, In : ACS Catalysis. 9, 4, p. 2962-2968 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Guanosine Diphosphate Mannose
Hydro-Lyases
Dehydration
Sugars
Deoxy Sugars

Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol

Horvat, M., Fritsche, S., Kourist, R. & Winkler, M., 19 Mar 2019, In : ChemCatChem. p. 4171-4181

Research output: Contribution to journalArticleResearchpeer-review

carboxylates
Oxidoreductases
preparation
cells
aldehydes

Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis

Savino, S., Borg, A. J. E., Dennig, A., Pfeiffer, M., De Giorgi, F., Weber, H., Dubey, K. D., Rovira, C., Mattevi, A. & Nidetzky, B., 1 Dec 2019, In : Nature Catalysis. 2, 12, p. 1115-1123 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Biosynthesis
Sugars
Xylose
Uridine Diphosphate
Decarboxylation

Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

Lemmerer, M., Mairhofer, J., Lepak, A., Longus, K., Hahn, R. & Nidetzky, B., 1 Jun 2019, In : Biotechnology and Bioengineering. 116, 6, p. 1259-1268 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Recombinant proteins
Glycosyltransferases
Cell growth
Recombinant Proteins
Escherichia coli

Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH

Strohmeier, G., Eiteljörg, I., Schwarz, A. & Winkler, M., 26 Apr 2019, In : Chemistry - a European Journal. 25, 24, p. 6119-6123 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Adenosinetriphosphate
Aldehydes
NADP
Carboxylic Acids
Adenosine Triphosphate

Glycosynthase Principle Transformed into Biocatalytic Process Technology: Lacto- N-triose II Production with Engineered exo-Hexosaminidase

Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 7 Jun 2019, In : ACS Catalysis. 9, 6, p. 5503-5514 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Hexosaminidases
Glucosamine
Glycosylation
Lactose
Trisaccharides

Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, In : Catalysis Science & Technology. 9, 3, p. 842-853 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Nitriles
Proton transfer
Catalysis
Escherichia coli
Protons

Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, p. 5661-5665 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Hexosaminidases
Human Milk
Lactose
Oligosaccharides
Glycosylation

Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase

Zhong, C., Luley-Goedl, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology and Bioengineering. 116, 9, p. 2146-2155 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
cellodextrin phosphorylase
cellobiose phosphorylase
Polymerization
Solubility
Glucose

Random Mutagenesis-Driven Improvement of Carboxylate Reductase Activity Using an Amino Benzamidoxime-Mediated High-Throughput Assay

Schwendenwein, D., Ressmann, A. K., Dörr, M., Höhne, M., Mihovilovic, M. D., Bornscheuer, U. T., Rudroff, F. & Winkler, M., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2544-2549 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Mutagenesis
Carboxylic acids
Assays
Oxidoreductases
Throughput

Substrate-Independent High-Throughput Assay for the Quantification of Aldehydes

Ressmann, A. K., Schwendenwein, D., Leonhartsberger, S., Mihovilovic, M. D., Bornscheuer, U. T., Winkler, M. & Rudroff, F., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2538-2543 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Aldehydes
Carboxylic acids
Assays
Throughput
Substrates

The microenvironment in immobilized enzymes: Methods of characterization and its role in determining enzyme performance

Bolivar, J. M. & Nidetzky, B., 24 Sep 2019, In : Molecules . 24, 19, 3460.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Immobilized Enzymes
enzymes
Enzymes
Liquids
Ionic strength

Three-Enzyme Phosphorylase Cascade for Integrated Production of Short-Chain Cellodextrins

Zhong, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology Journal. 1900349.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Phosphorylases
Sucrose
Enzymes
Polymerization
Phosphates
2018

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

Valikhani, D., Bolivar, J. M., Dennig, A. & Nidetzky, B., 1 Oct 2018, In : Biotechnology and Bioengineering. 115, 10, p. 2416-2425 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucose 1-Dehydrogenase
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Bacillus megaterium
Glucose

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisán, J. M. & Nidetzky, B., 28 Feb 2018, In : ACS Applied Materials & Interfaces. 10, 8, p. 6858-6868 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Optical sensors
Proteins
pH sensors
Penicillin Amidase
Immobilized Enzymes

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

Research output: Contribution to journalArticleResearchpeer-review

decarboxylation
Carboxy-Lyases
fatty acids
Hydrocarbons
Fatty acids

Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from Lipomyces starkeyi

Rother, C., Gutmann, A., Gudiminchi, R., Weber, H., Lepak, A. & Nidetzky, B., 16 Mar 2018, In : ChemBioChem. 19, 6, p. 596-603 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Glucose-6-Phosphate
Phosphotransferases
Adenosine Diphosphate
Adenosine Triphosphate

Demystifying the Flow: Biocatalytic Reaction Intensification in Microstructured Enzyme Reactors

Bolivar, J. M., Valikhani, D. & Nidetzky, B., 9 Aug 2018, In : Biotechnology Journal.

Research output: Contribution to journalArticleResearchpeer-review

Immobilized Enzymes
sucrose phosphorylase
Enzymes
Biocatalysis
Biotransformation

Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

Liu, Y., Gong, R., Liu, X., Zhang, P., Zhang, Q., Cai, Y-S., Deng, Z., Winkler, M., Wu, J. & Chen, W., 2018, In : Microbial cell factories . 17, 10 p., 131.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

Jung, J. & Nidetzky, B., 1 Jan 2018, In : Journal of Biological Chemistry. 293, 10, p. 3720-3733 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Nitriles
Imines
Escherichia coli
Amines
Oxidoreductases

Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

Schmölzer, K., Lemmerer, M. & Nidetzky, B., 1 Mar 2018, In : Biotechnology and Bioengineering. 115, 3, p. 545-556 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Glycosyltransferases
Glucosides
Polyphenols
Phloretin
Glycosylation
adhesives
sulfates
drugs
aerodynamics
powders

l-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: Aeration and carbon source influence yields and productivities

Novy, V., Brunner, B. & Nidetzky, B., 11 Apr 2018, In : Microbial cell factories . 17, 1, 59.

Research output: Contribution to journalArticleResearchpeer-review

Xylose
Lactic acid
Yeast
Glucose
Saccharomyces cerevisiae
Cellulose 1,4-beta-Cellobiosidase
Cellulose
Hydrolysis
Cellulases
Enzymes

New flavanol O-glycosides in grape and wine

Zerbib, M., Mazauric, J. P., Meudec, E., Le Guernevé, C., Lepak, A., Nidetzky, B., Cheynier, V., Terrier, N. & Saucier, C., 15 Nov 2018, In : Food chemistry. 266, p. 441-448 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Wine
Catechin
Vitis
flavanols
Glycosides

Pressurized CO2 as a carboxylating agent for the biocatalytic: Ortho -carboxylation of resorcinol

Plasch, K., Hofer, G., Keller, W., Hay, S., Heyes, D. J., Dennig, A., Glueck, S. M. & Faber, K., 1 Jan 2018, In : Green chemistry. 20, 8, p. 1754-1759 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Carboxylation
Enzymes
enzyme
Benzoic Acid
Carboxy-Lyases

Process intensification for O2-dependent enzymatic transformations in continuous single-phase pressurized flow

Bolivar, J. M., Mannsberger, A., Thomsen, M. S., Tekautz, G. & Nidetzky, B., 4 Dec 2018, In : Biotechnology and Bioengineering.

Research output: Contribution to journalArticleResearchpeer-review

Oxidoreductases
Biotransformation
Catalase
Pressure
Glucose Oxidase

Single-Particle Studies to Advance the Characterization of Heterogeneous Biocatalysts

Benítez-Mateos, A. I., Nidetzky, B., Bolivar, J. M. & López-Gallego, F., 21 Feb 2018, In : ChemCatChem. 10, 4, p. 654-665 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Biocatalysts
Immobilized Enzymes
Enzymes
enzymes
Structural integrity

Understanding the silica-based sol-gel encapsulation mechanism of Thermomyces lanuginosus lipase: The role of polyethylenimine

Escobar, S., Bernal, C., Bolivar, J. M., Nidetzky, B., López-Gallego, F. & Mesa, M., 1 Apr 2018, In : Molecular Catalysis. p. 106-113 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Polyethyleneimine
Lipases
Lipase
Encapsulation
Silicon Dioxide

β-Glucosyl Fluoride as Reverse Reaction Donor Substrate and Mechanistic Probe of Inverting Sugar Nucleotide-Dependent Glycosyltransferases

Lepak, A., Gutmann, A. & Nidetzky, B., 5 Oct 2018, In : ACS Catalysis. 8, 10, p. 9148-9153 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Flavonoids
Glycosyltransferases
Diphosphates
Uridine
Nucleotides
2017

An ortho C-methylation/O-glycosylation motif on a hydroxy-coumarin scaffold, selectively installed by biocatalysis

Gutmann, A., Schiller, M., Gruber-Khadjawi, M. & Nidetzky, B., 5 Sep 2017, In : Organic & biomolecular chemistry. 15, p. 7917 7924 p.

Research output: Contribution to journalArticleResearchpeer-review

A Spring in Performance: Silica Nanosprings Boost Enzyme Immobilization in Microfluidic Channels

Valikhani, D., Bolivar, J. M., Viefhues, M., McIlroy, D. N., Vrouwe, E. X. & Nidetzky, B., 11 Oct 2017, In : ACS Applied Materials & Interfaces. 9, 40, p. 34641-34649 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Enzyme immobilization
Microfluidics
Silicon Dioxide
Enzymes
Silica

Biocatalytic Cascade of Polyphosphate Kinase and Sucrose Synthase for Synthesis of Nucleotide-Activated Derivatives of Glucose

Kulmer, S. T., Gutmann, A., Lemmerer, M. & Nidetzky, B., 2017, In : Advanced Synthesis & Catalysis. 359, p. 292-301

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Sugar (sucrose)
Nucleotides
Nucleosides
Glucose
Diphosphates

Diffusion of Single-Pass Transmembrane Receptors: From the Plasma Membrane into Giant Liposomes

Worch, R., Petrášek, Z., Schwille, P. & Weidemann, T., 1 Aug 2017, In : Journal of Membrane Biology. 250, 4, p. 393-406 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Liposomes
Cell Membrane
Unilamellar Liposomes
Membrane Proteins
Blood Proteins

Erratum: Enzyme discovery beyond homology: a unique hydroxynitrile lyase in the Bet v1 superfamily

Lanfranchi, E., Pavkov-Keller, T., Koehler, E-M., Diepold, M., Steiner, K., Darnhofer, B., Hartler, J., Bergh, T. V. D., Joosten, H-J., Gruber-Khadjawi, M., Thallinger, G. G., Birner-Gruenberger, R., Gruber, K., Winkler, M. & Glieder, A., 26 May 2017, In : Scientific reports. 7, 46834.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
mandelonitrile lyase
Enzymes

Glycosyltransferase cascades for natural product glycosylation: Use of plant instead of bacterial sucrose synthases improves the UDP-glucose recycling from sucrose and UDP

Gutmann, A., Lepak, A., Diricks, M., Desmet, T. & Nidetzky, B., 2017, In : Biotechnology Journal.

Research output: Contribution to journalArticleResearchpeer-review

Uridine Diphosphate Glucose
Glycosyltransferases
Uridine Diphosphate
Recycling
Biological Products

Integrated process design for biocatalytic synthesis by a Leloir Glycosyltransferase: UDP-glucose production with sucrose synthase

Gutmann, A., 2017, In : Biotechnology and Bioengineering. p. 924-928

Research output: Contribution to journalArticleResearchpeer-review

Uridine Diphosphate Glucose
Glycosyltransferases
Sugar (sucrose)
Biotransformation
Glucose

Integration of whole-cell reaction and product isolation: Highly hydrophobic solvents promote in situ substrate supply and simplify extractive product isolation

Leis, D., Lauß, B., Macher-Ambrosch, R., Andreas, P., Nidetzky, B. & Kratzer, R., 2017, In : Journal of Biotechnology. 257, p. 110-117

Research output: Contribution to journalArticleResearchpeer-review

IN VIVO SYNTHESIS OF POLYHYDROXYLATED COMPOUNDS FROM A ‘HIDDEN RESERVOIR’ OF TOXIC ALDEHYDE SPECIES

Bayer, T., Wiesinger, T., Milker, S., Winkler, M., Mihovilovic, M. D. & Rudroff, F., 2017, In : ChemCatChem. 9, p. 2919-2923

Research output: Contribution to journalArticleResearchpeer-review

Isotope Probing of the UDP-Apiose/UDP-Xylose Synthase Reaction: Evidence of a Mechanism via a Coupled Oxidation and Aldol Cleavage

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie / International Edition . 56, p. 2503 –2507

Research output: Contribution to journalArticleResearchpeer-review

Xylose
Uridine Diphosphate
Isotopes
Enzymes
Sugars

Multivalency Effects on the Immobilization of Sucrose Phosphorylase in Flow Microchannels and Their Use in the Development of a High-Performance Biocatalytic Microreactor

Valikhani, D., Bolivar, J. M., Pfeiffer, M. & Nidetzky, B., 9 Jan 2017, In : ChemCatChem. 9, 1, p. 161-166 6 p.

Research output: Contribution to journalArticleResearchpeer-review

sucrose phosphorylase
sucrose
Sugar (sucrose)
microchannels
Microchannels

Production of glucosyl glycerol by immobilized sucrose phosphorylase: Options for enzyme fixation on a solid support and application in microscale flow format

Bolivar, J. M., Luley-Goedl, C., Leitner, E., Sawangwan, T. & Nidetzky, B., 10 Sep 2017, In : Journal of Biotechnology. 257, p. 131-138 8 p.

Research output: Contribution to journalArticleResearchpeer-review

sucrose phosphorylase
Sugar (sucrose)
Glycerol
Catalyst supports
Enzymes

Reaktion von UDP-Apiose/UDP-Xylose-Synthase mit isotopenmarkierten Substraten: Hinweise auf einen Mechanismus mit gekoppelter Oxidation und Aldolspaltung

Eixelsberger, T., Horvat, D., Gutmann, A., Weber, H. & Nidetzky, B., 2017, In : Angewandte Chemie. 129, p. 2544 –2548

Research output: Contribution to journalArticleResearchpeer-review

Sequence determinants of nucleotide binding in Sucrose Synthase: improving the affinity of a bacterial Sucrose Synthase for UDP by introducing plant residues

Diricks, M., Gutmann, A., Debacker, S., Dewitte, G., Nidetzky, B. & Desmet, T., 2017, In : Protein engineering, design and selection. 30, 3, p. 141-148

Research output: Contribution to journalArticleResearchpeer-review

Uridine Diphosphate
Sugar (sucrose)
Nucleotides
Administrative data processing
Diphosphates

Single-molecule study of oxidative enzymaticdeconstruction of cellulose

Eibinger, M., Sattelkow, J., Ganner, T., Plank, H. & Nidetzky, B., 2017, In : Nature Communications . p. 8:894 7 p.

Research output: Contribution to journalArticleResearch