• 8010

    Petersgasse 10-12 Graz

    Austria

Research Output 1966 2020

2020

Enzyme Immobilization in Wall-Coated Flow Microreactors

Valikhani, D., Bolivar, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 243-257 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Immobilization
Enzymes
sucrose phosphorylase
Silicon Dioxide
Glass

Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications

Wiltschi, B., Cernava, T., Dennig, A., Galindo, M., Geier, M., Gruber, S., Haberbauer, M., Heidinger, P., Acero, E. H., Kratzer, R., Luley-Goedl, C., Müller, C. A., Pitzer, J., Ribitsch, D., Sauer, M., Schmölzer, K., Schnitzhofer, W., Sensen, C. W., Soh, J., Steiner, K. & 3 othersWinkler, C. K., Winkler, M. & Wriessnegger, T., 22 Jan 2020, In : Biotechnology Advances. p. 107520

Research output: Contribution to journalArticleResearchpeer-review

Enzymes
Recovery
Biocontrol
Bioconversion
Biocatalysts

Intraparticle pH Sensing Within Immobilized Enzymes: Immobilized Yellow Fluorescent Protein as Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Particles

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisan, J. M. & Nidetzky, B., 1 Jan 2020, In : Methods in molecular biology (Clifton, N.J.). 2100, p. 319-333 15 p.

Research output: Contribution to journalArticleResearchpeer-review

Immobilized Enzymes
Proteins
Enzymes
Penicillin Amidase
Proton-Motive Force
Immobilized Enzymes
Molecules
Enzymes
Immobilization
Catalytic Domain
2019

3-Hydroxytyrosol: A whole cell mediated Bioreduction using a type IV Carboxylate Reductase

Horvat, M. & Winkler, M., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

A cell-free in vitro reduction of carboxylate to aldehyde

Schwarz, A., Strohmeier, G., Eiteljörg, I., Kohrt, J., Howard, R. & Winkler, M., Nov 2019.

Research output: Contribution to conferencePosterResearch

Adsorption and desorption of self-assembled L-cysteine monolayers on nanoporous gold monitored by in situ resistometry

Hengge, E., Steyskal, E-M., Bachler, R., Dennig, A., Nidetzky, B. & Würschum, R., 18 Nov 2019, In : Beilstein Journal of Nanotechnology . 10, p. 2275-2279 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
cysteine
Gold
Cysteine
Monolayers
Desorption

A Multi-Enzyme System for the In vitro Reduction of Carboxylic Acids to Aldehydes

Strohmeier, G., Schwarz, A., Eiteljörg, I., Kohrt, J., Howard, R. & Winkler, M., Jul 2019.

Research output: Contribution to conferencePosterResearch

An integrated eco-system for pollution prevention and greening the production chain of small-scale rice-paper production: A case study from Vietnam

Hai, L. T., Thanh Tran, V., Vi, L. Q., Phuong Thao, N. T., Hieu, T. T., Braunegg, S., Schnitzer, H. & Braunegg, G., 9 Oct 2019, In : Journal of cleaner production. 28 p., 118785.

Research output: Contribution to journalArticleResearchpeer-review

Exhaust gases
Ashes
Farms
Wastewater
Pollution

A Parsimonious Mechanism of Sugar Dehydration by Human GDP-Mannose-4,6-dehydratase

Pfeiffer, M., Johansson, C., Krojer, T., Kavanagh, K. L., Oppermann, U. & Nidetzky, B., 5 Apr 2019, In : ACS Catalysis. 9, 4, p. 2962-2968 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Guanosine Diphosphate Mannose
Hydro-Lyases
Dehydration
Sugars
Deoxy Sugars

Characterization of carboxylate reductases (CARs) for the reduction of aromatic carboxylic acids with integrated in vitro cofactor recycling

Weber, D., Patsch, D., Winkler, M., Turner, N. J. & Rother, D., Nov 2019.

Research output: Contribution to conferencePosterResearch

Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol

Horvat, M., Fritsche, S., Kourist, R. & Winkler, M., 19 Mar 2019, In : ChemCatChem. p. 4171-4181

Research output: Contribution to journalArticleResearchpeer-review

carboxylates
Oxidoreductases
preparation
cells
aldehydes

Comparison of two new and a known Type IV fungal Carboxylate reductase

Horvat, M., Fritsche, S. & Winkler, M., Nov 2019.

Research output: Contribution to conferencePosterResearch

Open Access
Organic Chemistry
Hexosaminidases
organic chemistry
chemistry
synthesis

Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Rinnofner, C., Kerschbaumer, B., Weber, H., Glieder, A. & Winkler, M., Nov 2019.

Research output: Contribution to conferencePosterResearch

Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Rinnofner, C., Kerschbaumer, B., Weber, H., Glieder, A. & Winkler, M., Apr 2019.

Research output: Contribution to conferencePosterResearch

Deciphering the enzymatic mechanism of sugar ring contraction in UDP-apiose biosynthesis

Savino, S., Borg, A. J. E., Dennig, A., Pfeiffer, M., De Giorgi, F., Weber, H., Dubey, K. D., Rovira, C., Mattevi, A. & Nidetzky, B., 1 Dec 2019, In : Nature Catalysis. 2, 12, p. 1115-1123 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Biosynthesis
Sugars
Xylose
Uridine Diphosphate
Decarboxylation

Decoupling of recombinant protein production from Escherichia coli cell growth enhances functional expression of plant Leloir glycosyltransferases

Lemmerer, M., Mairhofer, J., Lepak, A., Longus, K., Hahn, R. & Nidetzky, B., 1 Jun 2019, In : Biotechnology and Bioengineering. 116, 6, p. 1259-1268 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Recombinant proteins
Glycosyltransferases
Cell growth
Recombinant Proteins
Escherichia coli

Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH

Strohmeier, G., Eiteljörg, I., Schwarz, A. & Winkler, M., 26 Apr 2019, In : Chemistry - a European Journal. 25, 24, p. 6119-6123 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Adenosinetriphosphate
Aldehydes
NADP
Carboxylic Acids
Adenosine Triphosphate

Glycosynthase Principle Transformed into Biocatalytic Process Technology: Lacto- N-triose II Production with Engineered exo-Hexosaminidase

Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 7 Jun 2019, In : ACS Catalysis. 9, 6, p. 5503-5514 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Hexosaminidases
Glucosamine
Glycosylation
Lactose
Trisaccharides

High-Precision rapid sampling device

Klimacek, M., Scharfetter, H. & Hilber, D., 23 Oct 2019, IPC No. EP 3 556 843 A1, Patent No. 18168285.7

Research output: PatentResearch

Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

Jung, J., Braun, J., Czabany, T. & Nidetzky, B., 14 Jan 2019, In : Catalysis Science & Technology. 9, 3, p. 842-853 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Nitriles
Proton transfer
Catalysis
Escherichia coli
Protons

Lacto-: N -tetraose synthesis by wild-type and glycosynthase variants of the β- N -hexosaminidase from Bifidobacterium bifidum

Schmölzer, K., Weingarten, M., Baldenius, K. & Nidetzky, B., 1 Jan 2019, In : Organic and Biomolecular Chemistry. 17, 23, p. 5661-5665 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Hexosaminidases
Human Milk
Lactose
Oligosaccharides
Glycosylation

Nitrile hydratasees for synthesis of amides from nitriles

Grill, B., Schwab, H., Pogorevcnik, U., Glänzer, M., Reicher, K., Donsbach, K. & Winkler, M., Nov 2019.

Research output: Contribution to conferencePosterResearch

Optimizing the heterologous expression of the carboxylic acid reductase from Nocardia otitidiscavium for the reduction of aromatic acids

Weber, D., Patsch, D., Winkler, M., Turner, N. & Rother, D., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

Product solubility control in cellooligosaccharide production by coupled cellobiose and cellodextrin phosphorylase

Zhong, C., Luley-Goedl, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology and Bioengineering. 116, 9, p. 2146-2155 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
cellodextrin phosphorylase
cellobiose phosphorylase
Polymerization
Solubility
Glucose

Random Mutagenesis-Driven Improvement of Carboxylate Reductase Activity Using an Amino Benzamidoxime-Mediated High-Throughput Assay

Schwendenwein, D., Ressmann, A. K., Dörr, M., Höhne, M., Mihovilovic, M. D., Bornscheuer, U. T., Rudroff, F. & Winkler, M., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2544-2549 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Mutagenesis
Carboxylic acids
Assays
Oxidoreductases
Throughput

Random mutagenesis of carboxylate reductase enabled by a substrate independent HT-assay

Rudroff, F., Winkler, M., Schwendenwein, D., Ressmann, A. K., Mihovilovic, M. D., Bornscheuer, U. T. & Dörr, M., Jul 2019.

Research output: Contribution to conferencePosterResearch

Structure elucidation of the carboxylate reductase from Neurospora crassa (NcCAR)

Daniel, B., Pavkov-Keller, T., Leithold, M., Gruber, K., Stolterfoht, H. & Winkler, M., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

Substrate-Independent High-Throughput Assay for the Quantification of Aldehydes

Ressmann, A. K., Schwendenwein, D., Leonhartsberger, S., Mihovilovic, M. D., Bornscheuer, U. T., Winkler, M. & Rudroff, F., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2538-2543 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Aldehydes
Carboxylic acids
Assays
Throughput
Substrates

The influence of feedstock characteristics on enzyme production in Trichoderma reesei: A review on productivity, gene regulation and secretion profiles

Novy, V., Nielsen, F., Seiboth, B. & Nidetzky, B., 1 Jan 2019, In : Biotechnology for Biofuels. 12, 1, 238.

Research output: Contribution to journalReview articleResearchpeer-review

Open Access
Trichoderma
Gene expression
secretion
Feedstocks
Enzymes

The microenvironment in immobilized enzymes: Methods of characterization and its role in determining enzyme performance

Bolivar, J. M. & Nidetzky, B., 24 Sep 2019, In : Molecules . 24, 19, 3460.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Immobilized Enzymes
enzymes
Enzymes
Liquids
Ionic strength

Three-Enzyme Phosphorylase Cascade for Integrated Production of Short-Chain Cellodextrins

Zhong, C. & Nidetzky, B., 1 Jan 2019, In : Biotechnology Journal. 1900349.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Phosphorylases
Sucrose
Enzymes
Polymerization
Phosphates

Towards a new panel of nitrile hydratases

Grill, B., Schwab, H., Glänzer, M., Steinkellner, G., Strohmeier, G., Pogorevcnik, U., Reicher, K., Gruber, K., Donsbach, K. & Winkler, M., 26 Jun 2019.

Research output: Contribution to conferencePosterResearch

2018

A novel assay for the detection of carboxylic acid reductase (CAR) activity in whole cells

Schwendenwein, D., Ressmann, A. K., Mihovilovic, M. D., Bornscheuer, U. T., Dörr, M., Rudroff, F. & Winkler, M., 27 Aug 2018.

Research output: Contribution to conferencePosterResearch

A tailor-made, self-sufficient and recyclable monooxygenase catalyst based on coimmobilized cytochrome P450 BM3 and glucose dehydrogenase

Valikhani, D., Bolivar, J. M., Dennig, A. & Nidetzky, B., 1 Oct 2018, In : Biotechnology and Bioengineering. 115, 10, p. 2416-2425 10 p.

Research output: Contribution to journalArticleResearchpeer-review

Glucose 1-Dehydrogenase
Mixed Function Oxygenases
Cytochrome P-450 Enzyme System
Bacillus megaterium
Glucose

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisán, J. M. & Nidetzky, B., 28 Feb 2018, In : ACS Applied Materials & Interfaces. 10, 8, p. 6858-6868 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Optical sensors
Proteins
pH sensors
Penicillin Amidase
Immobilized Enzymes

Bio-based α,ω functionalized hydrocarbons from multi-step reaction sequences with bio- and metallo-catalysts based on the fatty acid decarboxylase OleTJE

Bojarra, S., Reichert, D., Grote, M., Gómez Baraibar, Á., Dennig, A., Nidetzky, B., Mügge, C. & Kourist, R., Mar 2018, In : ChemCatChem. 10, 5, p. 1192-1201

Research output: Contribution to journalArticleResearchpeer-review

decarboxylation
Carboxy-Lyases
fatty acids
Hydrocarbons
Fatty acids

Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from Lipomyces starkeyi

Rother, C., Gutmann, A., Gudiminchi, R., Weber, H., Lepak, A. & Nidetzky, B., 16 Mar 2018, In : ChemBioChem. 19, 6, p. 596-603 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Glucose-6-Phosphate
Phosphotransferases
Adenosine Diphosphate
Adenosine Triphosphate

Demystifying the Flow: Biocatalytic Reaction Intensification in Microstructured Enzyme Reactors

Bolivar, J. M., Valikhani, D. & Nidetzky, B., 9 Aug 2018, In : Biotechnology Journal.

Research output: Contribution to journalArticleResearchpeer-review

Immobilized Enzymes
sucrose phosphorylase
Enzymes
Biocatalysis
Biotransformation

Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

Liu, Y., Gong, R., Liu, X., Zhang, P., Zhang, Q., Cai, Y-S., Deng, Z., Winkler, M., Wu, J. & Chen, W., 2018, In : Microbial cell factories . 17, 10 p., 131.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Evidence of a sequestered imine intermediate during reduction of nitrile to amine by the nitrile reductase QueF from Escherichia coli

Jung, J. & Nidetzky, B., 1 Jan 2018, In : Journal of Biological Chemistry. 293, 10, p. 3720-3733 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Nitriles
Imines
Escherichia coli
Amines
Oxidoreductases

Fatty Alcohols: enzymatically from Free fatty Acids

Horvat, M., Zabielska, J., Kourist, R. & Winkler, M., 3 Jul 2018.

Research output: Contribution to conferencePosterResearch

Fragrance synthesis via a chemo- & multi enzyme cascade

Rudroff, F., Winkler, M., Entner, M., Schwendenwein, D., Ressmann, A. K. & Mihovilovic, M. D., Jul 2018.

Research output: Contribution to conferencePosterResearch

Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

Schmölzer, K., Lemmerer, M. & Nidetzky, B., 1 Mar 2018, In : Biotechnology and Bioengineering. 115, 3, p. 545-556 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Glycosyltransferases
Glucosides
Polyphenols
Phloretin
Glycosylation

Humane Enzyme für die Organische Synthese

Winkler, M., Geier, M., Hanlon, S. P., Nidetzky, B. & Glieder, A., 2018, In : Angewandte Chemie. 130, p. 13592-13610

Research output: Contribution to journalReview articleResearchpeer-review

Human Enzymes for Organic Synthesis

Winkler, M., Geier, M., Hanlon, S. P., Nidetzky, B. & Glieder, A., 2018, In : Angewandte Chemie / International Edition . 57, 41, p. 13406-13423 18 p.

Research output: Contribution to journalReview articleResearchpeer-review

Enzymes
Pharmaceutical Preparations
Xenobiotics
Metabolites
Metabolism
adhesives
sulfates
drugs
aerodynamics
powders

Leloir Glycosyltransferases as Biocatalysts for Chemical Production

Nidetzky, B., Gutmann, A. & Zhong, C., 6 Jul 2018, In : ACS Catalysis. 8, 7, p. 6283-6300 18 p.

Research output: Contribution to journalReview articleResearchpeer-review

Glycosylation
Glycosyltransferases
Biocatalysts
Enzymes
Nucleotides