ACIB – Austrian Center of Industrial Biotechnology (98730)

  • 8010

    Krenngasse 37 Graz

    Austria

Research Output 1990 2019

2019

3-Hydroxytyrosol: A whole cell mediated Bioreduction using a type IV Carboxylate Reductase

Horvat, M. & Winkler, M., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

A functionally-distinct carboxylic acid reductase (PcCAR4) unearthed from a repertoire of type IV CARs in the white-rot fungus Pycnoporus cinnabarinus

Ling, J. G., Mansor, M. H., Murad, M. A., Khalid, R. M., Quay, D. H-X., Winkler, M. & Bakar, F. D. A., 2019, In : Journal of Biotechnology. 2020, 307, p. 55-62

Research output: Contribution to journalArticleResearchpeer-review

Pycnoporus
Fungi
Carboxylic acids
Genes
Enzymes

A Multi-Enzyme System for the In vitro Reduction of Carboxylic Acids to Aldehydes

Strohmeier, G., Schwarz, A., Eiteljörg, I., Kohrt, J., Howard, R. & Winkler, M., Jul 2019.

Research output: Contribution to conferencePosterResearch

Capsaicin Metabolite Synthesis by Fungal Cytochrome P450

Willim, M., Bachler, T., Schweda, B., Migglautsch, A. K., Glieder, A., Breinbauer, R. & Winkler, M., 14 Feb 2019.

Research output: Contribution to conferencePosterResearch

Characterization of Type IV Carboxylate Reductases (CARs) for Whole Cell-Mediated Preparation of 3-Hydroxytyrosol

Horvat, M., Fritsche, S., Kourist, R. & Winkler, M., 19 Mar 2019, In : ChemCatChem. p. 4171-4181

Research output: Contribution to journalArticleResearchpeer-review

carboxylates
Oxidoreductases
preparation
cells
aldehydes

Co-factor demand and regeneration in the enzymatic one-step reduction of carboxylates to aldehydes in cell-free systems

Strohmeier, G., Schwarz, A., Andexer, J. N. & Winkler, M., 28 Oct 2019, (Accepted/In press) In : Journal of Biotechnology. 14 p.

Research output: Contribution to journalArticleResearchpeer-review

Cell-Free System
Aldehydes
Regeneration
Adenosine Triphosphate
Sinorhizobium meliloti

Cytochrome P450 mediated hydroxylation of ibuprofen using Pichia pastoris as biocatalyst

Rinnofner, C., Kerschbaumer, B., Weber, H., Glieder, A. & Winkler, M., 2019, In : Biocatalysis and Agricultural Biotechnology. 17, p. 525-528 4 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
ibuprofen
Hydroxylation
Pichia pastoris
Biocatalysts
Pichia

Discovery of carboxylic acid reductase (CAR) from Thermothelomyces thermophilus and its evaluation for vanillin synthesis

Horvat, M., Fiume, G., Fritsche, S. & Winkler, M., 12 Aug 2019, In : Journal of Biotechnology. 304, p. 44-51

Research output: Contribution to journalArticleResearchpeer-review

Carboxylic acids
Substrates
Vanillic Acid
Eugenol
Acids

Disease incidence in sugar beet fields is correlated with microbial diversity and distinct biological markers

Kusstatscher, P., Cernava, T., Harms, K., Maier, J., Eigner, H., Berg, G. & Zachow, C., 4 Mar 2019, In : Phytobiomes Journal.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
sugar beet
disease incidence
biomarkers
beets
root rot

Enzymatic One-Step Reduction of Carboxylates to Aldehydes with Cell-Free Regeneration of ATP and NADPH

Strohmeier, G., Eiteljörg, I., Schwarz, A. & Winkler, M., 26 Apr 2019, In : Chemistry - A European Journal. 25, 24, p. 6119-6123 5 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access
Adenosinetriphosphate
Aldehydes
NADP
Carboxylic Acids
Adenosine Triphosphate

Enzyme-catalyzed synthesis of amides from nitriles

Glänzer, M., Grill, B., Schwab, H., Pogorevcnik, U., Reicher, K., Donsbach, K., Pienaar, D. & Winkler, M., Sep 2019.

Research output: Contribution to conferencePosterResearch

Exploring the biocatalytic potential of a cytochrome P450 from Thermothelomyces thermophila

Fürst, M. J. L. J., Kerschbaumer, B., Rinnofner, C., Migglautsch, A. K., Winkler, M. & Fraaije, M. W., 2019, (Accepted/In press) In : Advanced Synthesis & Catalysis. p. 2487 2496 p.

Research output: Contribution to journalArticleResearchpeer-review

Hydrogen-driven cofactor regeneration for stereoselective whole-cell C=C bond reduction in Cupriavidus necator

Assil Companioni, L., Schmidt, S., Heidinger, P., Schwab, H. & Kourist, R., 19 Mar 2019, In : ChemSusChem. 12, 11, p. 2361-2365 5 p.

Research output: Contribution to journalArticleResearchpeer-review

biotransformation
Hydrogen
regeneration
hydrogen
Hydrogenase

Membrane protein production in yeast: Modification of yeast membranes for human membrane protein production

Emmerstorfer-Augustin, A., Wriessnegger, T., Hirz, M., Zellnig, G. & Pichler, H., 9 Feb 2019, Recombinant Protein Production in Yeast: Methods and Protocols. Springer, (Methods in Molecular Biology).

Research output: Chapter in Book/Report/Conference proceedingChapterResearchpeer-review

Microbiome-driven identification of microbial indicators for postharvest diseases of sugar beets

Kusstatscher, P., Zachow, C., Harms, K., Maier, J., Eigner, H., Berg, G. & Cernava, T., 7 Aug 2019, In : Microbiome.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Optimizing the heterologous expression of the carboxylic acid reductase from Nocardia otitidiscavium for the reduction of aromatic acids

Weber, D., Patsch, D., Winkler, M., Turner, N. & Rother, D., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

Random Mutagenesis-Driven Improvement of Carboxylate Reductase Activity Using an Amino Benzamidoxime-Mediated High-Throughput Assay

Schwendenwein, D., Ressmann, A. K., Dörr, M., Höhne, M., Mihovilovic, M. D., Bornscheuer, U. T., Rudroff, F. & Winkler, M., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2544-2549 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Mutagenesis
Carboxylic acids
Assays
Oxidoreductases
Throughput

Random mutagenesis of carboxylate reductase enabled by a substrate independent HT-assay

Rudroff, F., Winkler, M., Schwendenwein, D., Ressmann, A. K., Mihovilovic, M. D., Bornscheuer, U. T. & Dörr, M., Jul 2019.

Research output: Contribution to conferencePosterResearch

Recombinant lipoxygenases (LOX) and hydroperoxide lyases (HPL) for the synthesis of green leaf volatiles

Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., 8 Oct 2019, (Accepted/In press) In : Journal of agricultural and food chemistry. 59 p.

Research output: Contribution to journalReview articleResearchpeer-review

Lipoxygenases
lyases
hydroperoxides
lipoxygenase
synthesis

Recombinant lipoxygenases and hydroperoxide lyases for the synthesis of green leaf volatiles

Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., 2019, (Accepted/In press) In : Journal of agricultural and food chemistry.

Research output: Contribution to journalReview articleResearchpeer-review

Signature taxa indicate the storability of sugar beets before harvest

Kusstatscher, P., Cernava, T., Harms, K., Maier, J., Eigner, H., Berg, G. & Zachow, C., 2019.

Research output: Contribution to conferencePosterResearchpeer-review

Structure elucidation of the carboxylate reductase from Neurospora crassa (NcCAR)

Daniel, B., Pavkov-Keller, T., Leithold, M., Gruber, K., Stolterfoht, H. & Winkler, M., 9 Jul 2019.

Research output: Contribution to conferencePosterResearch

Substrate-Independent High-Throughput Assay for the Quantification of Aldehydes

Ressmann, A. K., Schwendenwein, D., Leonhartsberger, S., Mihovilovic, M. D., Bornscheuer, U. T., Winkler, M. & Rudroff, F., 6 Jun 2019, In : Advanced Synthesis & Catalysis. 361, 11, p. 2538-2543 6 p.

Research output: Contribution to journalArticleResearchpeer-review

Aldehydes
Carboxylic acids
Assays
Throughput
Substrates

Towards a new panel of nitrile hydratases

Grill, B., Schwab, H., Glänzer, M., Steinkellner, G., Strohmeier, G., Pogorevcnik, U., Reicher, K., Gruber, K., Donsbach, K. & Winkler, M., 26 Jun 2019.

Research output: Contribution to conferencePosterResearch

Valorization of Fatty Acids by Pichia pastoris: A Fresh Approach for Green Leaf Volatile Synthesis

Hashem, C., Steinberger, S., Stolterfoht, H., Rinnofner, C., Winkler, M. & Pichler, H., 12 Nov 2019.

Research output: Contribution to conferencePosterResearch

2018

Aliphatic hydroxylation and epoxidation of capsaicin by Cytochrome P450 CYP505X

Migglautsch, A. K., Willim, M., Schweda, B., Glieder, A., Breinbauer, R. & Winkler, M., 31 Aug 2018, In : Tetrahedron. 74, p. 6199-6204

Research output: Contribution to journalArticleResearchpeer-review

A novel assay for the detection of carboxylic acid reductase (CAR) activity in whole cells

Schwendenwein, D., Ressmann, A. K., Mihovilovic, M. D., Bornscheuer, U. T., Dörr, M., Rudroff, F. & Winkler, M., 27 Aug 2018.

Research output: Contribution to conferencePosterResearch

Asymmetric Reductive Carbocyclization Using Engineered Ene Reductases

Heckenbichler, K., Schweiger, A. K., Brandner, L. A., Binter, A., Toplak, M., Macheroux, P., Gruber, K. & Breinbauer, R., 2018, In : Angewandte Chemie / International Edition . 57, p. 7240 –7244

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Asymmetrische reduktive Carbocyclisierung durch modifizierte En‐Reduktasen

Heckenbichler, K., Schweiger, A. K., Brandner, L. A., Binter, A., Toplak, M., Macheroux, P., Gruber, K. & Breinbauer, R., 2018, In : Angewandte Chemie. 130, p. 7360-7364

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Biobased, Internally pH-Sensitive Materials: Immobilized Yellow Fluorescent Protein as an Optical Sensor for Spatiotemporal Mapping of pH Inside Porous Matrices

Consolati, T., Bolivar, J. M., Petrasek, Z., Berenguer, J., Hidalgo, A., Guisán, J. M. & Nidetzky, B., 28 Feb 2018, In : ACS Applied Materials & Interfaces. 10, 8, p. 6858-6868 11 p.

Research output: Contribution to journalArticleResearchpeer-review

Optical sensors
Proteins
pH sensors
Penicillin Amidase
Immobilized Enzymes

Biochemical Characterization and Mechanistic Analysis of the Levoglucosan Kinase from Lipomyces starkeyi

Rother, C., Gutmann, A., Gudiminchi, R., Weber, H., Lepak, A. & Nidetzky, B., 16 Mar 2018, In : ChemBioChem. 19, 6, p. 596-603 8 p.

Research output: Contribution to journalArticleResearchpeer-review

Lipomyces
Glucose-6-Phosphate
Phosphotransferases
Adenosine Diphosphate
Adenosine Triphosphate

Biological postharvest control in sugar beet

Kusstatscher, P., Cernava, T., Harms, K., Maier, J., Eigner, H., Treffer, D., Berg, G. & Zachow, C., Apr 2018.

Research output: Contribution to conferencePosterResearchpeer-review

Carboxylic acid reductase enzymes (CARs)

Winkler, M., 2018, In : Current opinion in chemical biology. 43, p. 23-29

Research output: Contribution to journalReview articleResearchpeer-review

Combinatorial optimization strategies to increase Human P450 catalytic Activity in whole cell

Weninger, A., Alkofer, A., Schmid, C., Bitter, J., Volpini de Maestri, A., Pranjic, M., Winkler, M. & Glieder, A., Sep 2018.

Research output: Contribution to conferencePosterResearch

Discovery and characterization of the tubercidin biosynthetic pathway from Streptomyces tubercidicus NBRC 13090

Liu, Y., Gong, R., Liu, X., Zhang, P., Zhang, Q., Cai, Y-S., Deng, Z., Winkler, M., Wu, J. & Chen, W., 2018, In : Microbial cell factories . 17, 10 p., 131.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Effect of Noncanonical Amino Acids on Protein-Carbohydrate Interactions: Structure, Dynamics, and Carbohydrate Affinity of a Lectin Engineered with Fluorinated Tryptophan Analogs

Tobola, F., Lelimousin, M., Varrot, A., Gillon, E., Darnhofer, B., Blixt, O., Birner-Gruenberger, R., Imberty, A. & Wiltschi, B., 17 Aug 2018, In : ACS Chemical Biology. 13, 8, p. 2211-2219 9 p.

Research output: Contribution to journalArticleResearchpeer-review

Lectins
Tryptophan
Carbohydrates
Amino Acids
Proteins

Erratum to: Engineered bidirectional promoters enable rapid multi-gene co-expression optimization (Nature Communications, (2018), 9, 1, (3589), 10.1038/s41467-018-05915-w)

Vogl, T., Kickenweiz, T., Pitzer, J., Sturmberger, L., Weninger, A., Biggs, B. W., Köhler, E. M., Baumschlager, A., Fischer, J. E., Hyden, P., Wagner, M., Baumann, M., Borth, N., Geier, M., Ajikumar, P. K. & Glieder, A., 1 Dec 2018, In : Nature Communications. 9, 1, 4566.

Research output: Contribution to journalComment/debateResearchpeer-review

files
genes
Publications
Genes
communication

Exploring the plant microbiome for nonribosomal peptide synthetase diversity

Müller, C. A., Hollauf, S. N., Obermeier, M. M. & Berg, G., 3 Sep 2018.

Research output: Contribution to conferencePosterResearchpeer-review

Fatty Alcohols: enzymatically from Free fatty Acids

Horvat, M., Zabielska, J., Kourist, R. & Winkler, M., 3 Jul 2018.

Research output: Contribution to conferencePosterResearch

First evaluation of alkylpyrazine application as a novel method to decrease microbial contaminations in processed meat products

Schöck, M., Liebminger, S., Berg, G. & Cernava, T., 3 Apr 2018, In : AMB Express. 8, 54, 7 p.

Research output: Contribution to journalArticleResearchpeer-review

Open Access

Fragrance synthesis via a chemo- & multi enzyme cascade

Rudroff, F., Winkler, M., Entner, M., Schwendenwein, D., Ressmann, A. K. & Mihovilovic, M. D., Jul 2018.

Research output: Contribution to conferencePosterResearch

Glycosyltransferase cascades made fit for chemical production: Integrated biocatalytic process for the natural polyphenol C-glucoside nothofagin

Schmölzer, K., Lemmerer, M. & Nidetzky, B., 1 Mar 2018, In : Biotechnology and Bioengineering. 115, 3, p. 545-556 12 p.

Research output: Contribution to journalArticleResearchpeer-review

Glycosyltransferases
Glucosides
Polyphenols
Phloretin
Glycosylation

Humane Enzyme für die Organische Synthese

Winkler, M., Geier, M., Hanlon, S. P., Nidetzky, B. & Glieder, A., 2018, In : Angewandte Chemie. 130, p. 13592-13610

Research output: Contribution to journalReview articleResearchpeer-review

Hybrid CARs (carboxylate reductases)

Stolterfoht, H., Gradischnig, D. & Winkler, M., 10 Oct 2018.

Research output: Contribution to conferencePosterResearch

Identification of key residues for enzymatic carboxylate reduction

Stolterfoht, H., Steinkellner, G., Schwendenwein, D., Pavkov-Keller, T., Gruber, K. & Winkler, M., 12 Feb 2018, In : Frontiers in Microbiology . 9, 250.

Research output: Contribution to journalArticleResearchpeer-review

In the search for antimicrobials: functional metagenomics applied to plant microbiomes

Müller, C. A., Hollauf, S. N., Hesch, F., Graf, L. A. & Berg, G., 8 Nov 2018.

Research output: Contribution to conferencePosterResearchpeer-review

l-Lactic acid production from glucose and xylose with engineered strains of Saccharomyces cerevisiae: Aeration and carbon source influence yields and productivities

Novy, V., Brunner, B. & Nidetzky, B., 11 Apr 2018, In : Microbial cell factories . 17, 1, 59.

Research output: Contribution to journalArticleResearchpeer-review

Xylose
Lactic acid
Yeast
Glucose
Saccharomyces cerevisiae

Melatonin minimizes the impact of oxidative stress induced by hydrogen peroxide in Saccharomyces and Non-conventional yeast

Vázquez, J., Grillitsch, K., Daum, G., Mas, A., Torija, M. J. & Beltran, G., 20 Aug 2018, In : Frontiers in Microbiology . 9, AUG, 1933.

Research output: Contribution to journalArticleResearchpeer-review

Saccharomyces
Melatonin
Hydrogen Peroxide
Oxidative Stress
Yeasts

On the current role of hydratases in biocatalysis

Engleder, M. & Pichler, H., 15 Jul 2018, In : Applied Microbiology and Biotechnology. 102, p. 5841-5858

Research output: Contribution to journalArticleResearchpeer-review

Open Access