DescriptionThe sugar beet endophyte Pseudomonas poae RE*1-1-14 is a component of an antagonistic cocktail developed to control root rot diseases in sugar beet. Based on an extended screening process the microbial compilation also includes the rhizobacteria P. fluorescens L13-6-12 and Serratia plymuthica 3Re4-18. To assess cell numbers during the seed treatment procedure, subsequent storage of the inoculated seeds and, finally, in course of plant development, a quantitative real-time PCR method was established for the model strain P. poae RE*1-1-14. For cultivation-independent monitoring of the population dynamics of the strain, we developed a pair of SCAR (Sequence Characterized Amplified Regions) primer for a specific 580 bp fragment derived from a comparative U-PCR approach. In combination with a strainspecific probe, the primer set was suitable to be employed in a TaqMan® real-time PCR assay. The described system allows the accurate detection and quantification of the antagonist in sugar beet seed coatings and ad planta under greenhouse conditions.
|Period||24 Jun 2012 → 27 Jun 2012|
|Event title||IOBC WPRS Working group "Biological Control of Fungal and Bacterial Plant Phatogens|
|Degree of Recognition||International|