Development of a TaqMan® probe-based real-time PCR approach to track the biological control agent Pseudomonas poae RE* 1-1-14.

Berg, G. (Speaker), H. Pirker (Speaker), Müller, H. (Speaker), Ralf Tilcher (Speaker), Christin Zachow (Speaker)

Activity: Talk or presentationTalk at conference or symposiumScience to science

Description

The sugar beet endophyte Pseudomonas poae RE*1-1-14 is a component of an antagonistic cocktail developed to control root rot diseases in sugar beet. Based on an extended screening process the microbial compilation also includes the rhizobacteria P. fluorescens L13-6-12 and Serratia plymuthica 3Re4-18. To assess cell numbers during the seed treatment procedure, subsequent storage of the inoculated seeds and, finally, in course of plant development, a quantitative real-time PCR method was established for the model strain P. poae RE*1-1-14. For cultivation-independent monitoring of the population dynamics of the strain, we developed a pair of SCAR (Sequence Characterized Amplified Regions) primer for a specific 580 bp fragment derived from a comparative U-PCR approach. In combination with a strainspecific probe, the primer set was suitable to be employed in a TaqMan® real-time PCR assay. The described system allows the accurate detection and quantification of the antagonist in sugar beet seed coatings and ad planta under greenhouse conditions.
Period24 Jun 201227 Jun 2012
Event titleIOBC WPRS Working group "Biological Control of Fungal and Bacterial Plant Phatogens
Event typeConference
LocationReims, France
Degree of RecognitionInternational