Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: Reaction intermediates revealed by crystallography and mass spectrometry

Barbara S. Lang; Antonius C.F. Gorren; Gustav Oberdorfer; M. Verena Wenzl; Cristina M. Furdui; Leslie B. Poole; Bernd Mayer; Karl Gruber

doi:10.1074/jbc.M112.371716

Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: Reaction intermediates revealed by crystallography and mass spectrometry

Barbara S. Lang, Antonius C.F. Gorren, Gustav Oberdorfer, M. Verena Wenzl, Cristina M. Furdui, Leslie B. Poole, Bernd Mayer^*, Karl Gruber

^*Korrespondierende/r Autor/-in für diese Arbeit

Technische Universität Graz (90000)

Publikation: Beitrag in einer Fachzeitschrift › Artikel › Begutachtung

Abstract

Background: Aldehyde dehydrogenase-2 (ALDH2) catalyzes bioactivation of glyceryl trinitrate (GTN) resulting in vasodilation. The exact mechanism is still unclear. Results: Structures of ALDH2 in complex with GTN and of a thionitrate intermediate were obtained. Conclusion: The structures represent snapshots of the first reaction step of GTN bioactivation by ALDH2. Significance: The results provide new insight into the mechanism of vascular GTN bioactivation by ALDH2.

Originalsprache	englisch
Seiten (von - bis)	38124-38134
Seitenumfang	11
Fachzeitschrift	Journal of Biological Chemistry
Jahrgang	287
Ausgabenummer	45
DOIs	https://doi.org/10.1074/jbc.M112.371716
Publikationsstatus	Veröffentlicht - 2 Nov. 2012

ASJC Scopus subject areas

Biochemie
Molekularbiologie
Zellbiologie

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10.1074/jbc.M112.371716

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Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: Reaction intermediates revealed by crystallography and mass spectrometry. / Lang, Barbara S.; Gorren, Antonius C.F.; Oberdorfer, Gustav et al.
in: Journal of Biological Chemistry, Jahrgang 287, Nr. 45, 02.11.2012, S. 38124-38134.

Publikation: Beitrag in einer Fachzeitschrift › Artikel › Begutachtung

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title = "Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: Reaction intermediates revealed by crystallography and mass spectrometry",

abstract = "Background: Aldehyde dehydrogenase-2 (ALDH2) catalyzes bioactivation of glyceryl trinitrate (GTN) resulting in vasodilation. The exact mechanism is still unclear. Results: Structures of ALDH2 in complex with GTN and of a thionitrate intermediate were obtained. Conclusion: The structures represent snapshots of the first reaction step of GTN bioactivation by ALDH2. Significance: The results provide new insight into the mechanism of vascular GTN bioactivation by ALDH2.",

author = "Lang, {Barbara S.} and Gorren, {Antonius C.F.} and Gustav Oberdorfer and Wenzl, {M. Verena} and Furdui, {Cristina M.} and Poole, {Leslie B.} and Bernd Mayer and Karl Gruber",

year = "2012",

month = nov,

day = "2",

doi = "10.1074/jbc.M112.371716",

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T1 - Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2

T2 - Reaction intermediates revealed by crystallography and mass spectrometry

AU - Lang, Barbara S.

AU - Gorren, Antonius C.F.

AU - Oberdorfer, Gustav

AU - Wenzl, M. Verena

AU - Furdui, Cristina M.

AU - Poole, Leslie B.

AU - Mayer, Bernd

AU - Gruber, Karl

PY - 2012/11/2

Y1 - 2012/11/2

N2 - Background: Aldehyde dehydrogenase-2 (ALDH2) catalyzes bioactivation of glyceryl trinitrate (GTN) resulting in vasodilation. The exact mechanism is still unclear. Results: Structures of ALDH2 in complex with GTN and of a thionitrate intermediate were obtained. Conclusion: The structures represent snapshots of the first reaction step of GTN bioactivation by ALDH2. Significance: The results provide new insight into the mechanism of vascular GTN bioactivation by ALDH2.

AB - Background: Aldehyde dehydrogenase-2 (ALDH2) catalyzes bioactivation of glyceryl trinitrate (GTN) resulting in vasodilation. The exact mechanism is still unclear. Results: Structures of ALDH2 in complex with GTN and of a thionitrate intermediate were obtained. Conclusion: The structures represent snapshots of the first reaction step of GTN bioactivation by ALDH2. Significance: The results provide new insight into the mechanism of vascular GTN bioactivation by ALDH2.

UR - http://www.scopus.com/inward/record.url?scp=84868322214&partnerID=8YFLogxK

U2 - 10.1074/jbc.M112.371716

DO - 10.1074/jbc.M112.371716

M3 - Article

C2 - 22988236

AN - SCOPUS:84868322214

SN - 0021-9258

VL - 287

SP - 38124

EP - 38134

JO - Journal of Biological Chemistry

JF - Journal of Biological Chemistry

IS - 45

ER -

Vascular bioactivation of nitroglycerin by aldehyde dehydrogenase-2: Reaction intermediates revealed by crystallography and mass spectrometry

Abstract

ASJC Scopus subject areas

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