TY - JOUR
T1 - Utilization of desugarized sugar beet molasses for the production of poly(3-hydroxybutyrate) by halophilic Bacillus megaterium uyuni S29
AU - Schmid, Maximilian T.
AU - Song, Hyunjeong
AU - Raschbauer, Michaela
AU - Emerstorfer, Florian
AU - Omann, Markus
AU - Stelzer, Franz
AU - Neureiter, Markus
PY - 2019/11/1
Y1 - 2019/11/1
N2 - Desugarized sugar beet molasses is a high-salinity side stream after fractionation of beet molasses by chromatography. It could be demonstrated that this by-product of the sugar industry is a suitable substrate for the production of poly(3-hydroxybutyrate) with the halophilic bacterium Bacillus megaterium uyuni S29. Cell dry mass concentrations of up to 16.7 g L−1 with a P(3HB) content of 0.6 g g−1 were achieved after only 24 h of batch cultivation. Utilization of a desugarized sugar beet molasses medium resulted in a two- to threefold increase in biomass production compared to a conventional mineral based medium. Depending on the extraction method, the molecular mass (Mn) of the P(3HB) varied between 119 kDa and 722 kDa. The high P(3HB) productivity of 0.42 g L−1 h−1 during batch experiments combined with desugarized sugar beet molasses as inexpensive substrate suggests a simple and robust process for cost effective P(3HB) production.
AB - Desugarized sugar beet molasses is a high-salinity side stream after fractionation of beet molasses by chromatography. It could be demonstrated that this by-product of the sugar industry is a suitable substrate for the production of poly(3-hydroxybutyrate) with the halophilic bacterium Bacillus megaterium uyuni S29. Cell dry mass concentrations of up to 16.7 g L−1 with a P(3HB) content of 0.6 g g−1 were achieved after only 24 h of batch cultivation. Utilization of a desugarized sugar beet molasses medium resulted in a two- to threefold increase in biomass production compared to a conventional mineral based medium. Depending on the extraction method, the molecular mass (Mn) of the P(3HB) varied between 119 kDa and 722 kDa. The high P(3HB) productivity of 0.42 g L−1 h−1 during batch experiments combined with desugarized sugar beet molasses as inexpensive substrate suggests a simple and robust process for cost effective P(3HB) production.
KW - Bacillus megaterium
KW - Desugarized sugar beet molasses
KW - Halophilic fermentation
KW - Poly(3-hydroxybutyrate)
KW - Sugar industry by-product
UR - http://www.scopus.com/inward/record.url?scp=85070498419&partnerID=8YFLogxK
U2 - 10.1016/j.procbio.2019.08.001
DO - 10.1016/j.procbio.2019.08.001
M3 - Article
AN - SCOPUS:85070498419
SN - 1359-5113
VL - 86
SP - 9
EP - 15
JO - Process Biochemistry
JF - Process Biochemistry
ER -