Translin and Trax differentially regulate telomere-associated transcript homeostasis

Natalia Gomez-Escobar, Nasser Almobadel, Othman Alzahrani, Julia Feichtinger, Vicente Planells-Palop, Zafer Alshehri, Gerhard G Thallinger, Jane A Wakeman, Ramsay J McFarlane

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Translin and Trax proteins are highly conserved nucleic acid binding proteins that have been implicated in RNA regulation in a range of biological processes including tRNA processing, RNA interference, microRNA degradation during oncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts. Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomeric regions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstrate that human Translin and Trax also control telomere-associated transcript levels in human cells in a telomere-specific fashion.

Originalspracheenglisch
Seiten (von - bis)33809-20
Seitenumfang12
FachzeitschriftOncoTarget
Jahrgang7
Ausgabenummer23
DOIs
PublikationsstatusVeröffentlicht - 7 Jun 2016

Fingerprint

Telomere
Homeostasis
Schizosaccharomyces pombe Proteins
Biological Phenomena
Heterochromatin
Spermatogenesis
RNA Interference
Transfer RNA
MicroRNAs
Nucleic Acids
Carrier Proteins
Carcinogenesis
Genome
RNA
Mutation
Proteins

Schlagwörter

    Dies zitieren

    Gomez-Escobar, N., Almobadel, N., Alzahrani, O., Feichtinger, J., Planells-Palop, V., Alshehri, Z., ... McFarlane, R. J. (2016). Translin and Trax differentially regulate telomere-associated transcript homeostasis. OncoTarget , 7(23), 33809-20. https://doi.org/10.18632/oncotarget.9278

    Translin and Trax differentially regulate telomere-associated transcript homeostasis. / Gomez-Escobar, Natalia; Almobadel, Nasser; Alzahrani, Othman; Feichtinger, Julia; Planells-Palop, Vicente; Alshehri, Zafer; Thallinger, Gerhard G; Wakeman, Jane A; McFarlane, Ramsay J.

    in: OncoTarget , Jahrgang 7, Nr. 23, 07.06.2016, S. 33809-20.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    Gomez-Escobar, N, Almobadel, N, Alzahrani, O, Feichtinger, J, Planells-Palop, V, Alshehri, Z, Thallinger, GG, Wakeman, JA & McFarlane, RJ 2016, 'Translin and Trax differentially regulate telomere-associated transcript homeostasis' OncoTarget , Jg. 7, Nr. 23, S. 33809-20. https://doi.org/10.18632/oncotarget.9278
    Gomez-Escobar N, Almobadel N, Alzahrani O, Feichtinger J, Planells-Palop V, Alshehri Z et al. Translin and Trax differentially regulate telomere-associated transcript homeostasis. OncoTarget . 2016 Jun 7;7(23):33809-20. https://doi.org/10.18632/oncotarget.9278
    Gomez-Escobar, Natalia ; Almobadel, Nasser ; Alzahrani, Othman ; Feichtinger, Julia ; Planells-Palop, Vicente ; Alshehri, Zafer ; Thallinger, Gerhard G ; Wakeman, Jane A ; McFarlane, Ramsay J. / Translin and Trax differentially regulate telomere-associated transcript homeostasis. in: OncoTarget . 2016 ; Jahrgang 7, Nr. 23. S. 33809-20.
    @article{e5ec3348f8a8451ea6e948a49ed71d1d,
    title = "Translin and Trax differentially regulate telomere-associated transcript homeostasis",
    abstract = "Translin and Trax proteins are highly conserved nucleic acid binding proteins that have been implicated in RNA regulation in a range of biological processes including tRNA processing, RNA interference, microRNA degradation during oncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts. Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomeric regions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstrate that human Translin and Trax also control telomere-associated transcript levels in human cells in a telomere-specific fashion.",
    keywords = "Journal Article",
    author = "Natalia Gomez-Escobar and Nasser Almobadel and Othman Alzahrani and Julia Feichtinger and Vicente Planells-Palop and Zafer Alshehri and Thallinger, {Gerhard G} and Wakeman, {Jane A} and McFarlane, {Ramsay J}",
    year = "2016",
    month = "6",
    day = "7",
    doi = "10.18632/oncotarget.9278",
    language = "English",
    volume = "7",
    pages = "33809--20",
    journal = "OncoTarget",
    issn = "1949-2553",
    publisher = "Impact Journals, LLC",
    number = "23",

    }

    TY - JOUR

    T1 - Translin and Trax differentially regulate telomere-associated transcript homeostasis

    AU - Gomez-Escobar, Natalia

    AU - Almobadel, Nasser

    AU - Alzahrani, Othman

    AU - Feichtinger, Julia

    AU - Planells-Palop, Vicente

    AU - Alshehri, Zafer

    AU - Thallinger, Gerhard G

    AU - Wakeman, Jane A

    AU - McFarlane, Ramsay J

    PY - 2016/6/7

    Y1 - 2016/6/7

    N2 - Translin and Trax proteins are highly conserved nucleic acid binding proteins that have been implicated in RNA regulation in a range of biological processes including tRNA processing, RNA interference, microRNA degradation during oncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts. Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomeric regions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstrate that human Translin and Trax also control telomere-associated transcript levels in human cells in a telomere-specific fashion.

    AB - Translin and Trax proteins are highly conserved nucleic acid binding proteins that have been implicated in RNA regulation in a range of biological processes including tRNA processing, RNA interference, microRNA degradation during oncogenesis, spermatogenesis and neuronal regulation. Here, we explore the function of this paralogue pair of proteins in the fission yeast. Using transcript analysis we demonstrate a reciprocal mechanism for control of telomere-associated transcripts. Mutation of tfx1+ (Trax) elevates transcript levels from silenced sub-telomeric regions of the genome, but not other silenced regions, such as the peri-centromeric heterochromatin. In the case of some sub-telomeric transcripts, but not all, this elevation is dependent on the Trax paralogue, Tsn1 (Translin). In a reciprocal fashion, Tsn1 (Translin) serves to repress levels of transcripts (TERRAs) from the telomeric repeats, whereas Tfx1 serves to maintain these elevated levels. This reveals a novel mechanism for the regulation of telomeric transcripts. We extend this to demonstrate that human Translin and Trax also control telomere-associated transcript levels in human cells in a telomere-specific fashion.

    KW - Journal Article

    U2 - 10.18632/oncotarget.9278

    DO - 10.18632/oncotarget.9278

    M3 - Article

    VL - 7

    SP - 33809

    EP - 33820

    JO - OncoTarget

    JF - OncoTarget

    SN - 1949-2553

    IS - 23

    ER -