TY - JOUR
T1 - Transcriptomic analysis of orange fruit treated with pomegranate peel extract (PGE)
AU - Belgacem, Imen
AU - Pangallo, Sonia
AU - Abdelfattah, Ahmed
AU - Romeo, Flora V.
AU - Cacciola, Santa O.
AU - Li Destri Nicosia, Maria G.
AU - Ballistreri, Gabriele
AU - Schena, Leonardo
PY - 2019/4/1
Y1 - 2019/4/1
N2 - A Pomegranate Peel Extract (PGE) has been proposed as a natural antifungal substance with a wide range of activity against plant diseases. Previous studies showed that the extract has a direct antimicrobial activity and can elicit resistance responses in plant host tissues. In the present study, the transcriptomic response of orange fruit toward PGE treatments was evaluated. RNA-seq analyses, conducted on wounded fruits 0, 6, and 24 h after PGE applications, showed a significantly di_erent transcriptome in treated oranges as compared to control samples. The majority (273) of the deferentially expressed genes (DEGs) were highly up-regulated compared to only 8 genes that were down-regulated. Gene Ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis showed the involvement of 1233 gene ontology (GO) terms and 35 KEGG metabolic pathways. Among these, important defense pathways were induced and antibiotic biosynthesis was the most enriched one. These findings may explain the underlying preventive and curative activity of PGE against plant diseases.
AB - A Pomegranate Peel Extract (PGE) has been proposed as a natural antifungal substance with a wide range of activity against plant diseases. Previous studies showed that the extract has a direct antimicrobial activity and can elicit resistance responses in plant host tissues. In the present study, the transcriptomic response of orange fruit toward PGE treatments was evaluated. RNA-seq analyses, conducted on wounded fruits 0, 6, and 24 h after PGE applications, showed a significantly di_erent transcriptome in treated oranges as compared to control samples. The majority (273) of the deferentially expressed genes (DEGs) were highly up-regulated compared to only 8 genes that were down-regulated. Gene Ontology (GO) and Kyoto encyclopedia of genes and genomes (KEGG) pathway enrichment analysis showed the involvement of 1233 gene ontology (GO) terms and 35 KEGG metabolic pathways. Among these, important defense pathways were induced and antibiotic biosynthesis was the most enriched one. These findings may explain the underlying preventive and curative activity of PGE against plant diseases.
KW - Orange
KW - PGE
KW - Plant defense
KW - Pomegranate peel extract
KW - RNA-seq
KW - Transcriptomics
UR - http://www.scopus.com/inward/record.url?scp=85067930451&partnerID=8YFLogxK
U2 - 10.3390/plants8040101
DO - 10.3390/plants8040101
M3 - Article
AN - SCOPUS:85067930451
SN - 2223-7747
VL - 8
JO - Plants
JF - Plants
IS - 4
M1 - 101
ER -