TY - JOUR
T1 - Surface structuring by Electron Beam for improved soft tissues adhesion and reduced bacterial contamination on Ti-grade 2
AU - Ferraris, S.
AU - Warchomicka, F.
AU - Ramskogler, C.
AU - Tortello, M.
AU - Cochis, A.
AU - Scalia, A.
AU - Gautier di Confiengo, G.
AU - Keckes, J.
AU - Rimondini, L.
AU - Spriano, S.
PY - 2019/4/1
Y1 - 2019/4/1
N2 - Even if soft tissue adhesion on titanium surfaces is an issue of great interest for transmucosal dental implants and percutaneous devices, few specific surface modification strategies have been approached so far to face it. Topographical surface modification is an interesting strategy because quality of soft tissues can be effectively improved by contact guidance effect on fibroblasts. Conversely, biofilm formation is encouraged in presence of rough or porous surfaces, as well as of surface patterns, if the roughness overcome the threshold of 0.2 μm. This issue becomes particularly critical in medical devices which cross different biological tissues and, through soft tissues, establish a direct contact with the external environment. In the present research, three different grooved patterns (5, 10 and 30 μm wide) were produced on commercially pure titanium by means of Electron Beam (EB) structuring maintaining the average surface roughness lower than 0.2 μm. The EB surface structured samples were characterized by means of optical and atomic force microscopy, contact and non-contact profilometry, X-Ray Diffraction, residual stress measurements as well as bacteria and cell adhesion tests. The 10 μm wide grooves resulted able to support fibroblast alignment onto the metallic substrate. Unexpectedly, all the EB surface structured surfaces not only did not enhance, but even reduced bacterial adhesion up to 48 h culture.
AB - Even if soft tissue adhesion on titanium surfaces is an issue of great interest for transmucosal dental implants and percutaneous devices, few specific surface modification strategies have been approached so far to face it. Topographical surface modification is an interesting strategy because quality of soft tissues can be effectively improved by contact guidance effect on fibroblasts. Conversely, biofilm formation is encouraged in presence of rough or porous surfaces, as well as of surface patterns, if the roughness overcome the threshold of 0.2 μm. This issue becomes particularly critical in medical devices which cross different biological tissues and, through soft tissues, establish a direct contact with the external environment. In the present research, three different grooved patterns (5, 10 and 30 μm wide) were produced on commercially pure titanium by means of Electron Beam (EB) structuring maintaining the average surface roughness lower than 0.2 μm. The EB surface structured samples were characterized by means of optical and atomic force microscopy, contact and non-contact profilometry, X-Ray Diffraction, residual stress measurements as well as bacteria and cell adhesion tests. The 10 μm wide grooves resulted able to support fibroblast alignment onto the metallic substrate. Unexpectedly, all the EB surface structured surfaces not only did not enhance, but even reduced bacterial adhesion up to 48 h culture.
KW - Bacterial adhesion
KW - Electron Beam
KW - Fibroblast alignment
KW - Oriented grooves
KW - Surface structuring
KW - Titanium
UR - http://www.scopus.com/inward/record.url?scp=85057265756&partnerID=8YFLogxK
U2 - 10.1016/j.jmatprotec.2018.11.026
DO - 10.1016/j.jmatprotec.2018.11.026
M3 - Article
AN - SCOPUS:85057265756
SN - 0924-0136
VL - 266
SP - 518
EP - 529
JO - Journal of Materials Processing Technology
JF - Journal of Materials Processing Technology
ER -