TY - JOUR
T1 - Structural study of (Hydroxypropyl)methyl cellulose microemulsion-based gels used for biocompatible encapsulations
AU - Vassiliadi, Evdokia
AU - Mitsou, Evgenia
AU - Avramiotis, Spyridon
AU - Chochos, Christos L.
AU - Pirolt, Franz
AU - Medebach, Martin
AU - Glatter, Otto
AU - Xenakis, Aristotelis
AU - Zoumpanioti, Maria
PY - 2020/11
Y1 - 2020/11
N2 - (Hydroxypropyl)methyl cellulose (HPMC) can be used to form gels integrating a w/o microemulsion. The formulation in which a microemulsion is mixed with a hydrated HPMC matrix has been successfully used as a carrier of biocompatible ingredients. However, little is known about the structure of these systems. To elucidate this, scanning electron microscopy was used to examine the morphology and the bulk of the microemulsion-based gels (MBGs) and small-angle X-ray scattering to clarify the structure and detect any residual reverse micelles after microemulsion incorporation in the gel. Electron paramagnetic resonance spectroscopy was applied using spin probes to investigate the polar and non-polar areas of the gel. Furthermore, the enzyme-labelling technique was followed to investigate the location of an enzyme in the matrix. A structural model for HPMC matrix is proposed according to which, although a w/o microemulsion is essential to form the final gel, no microemulsion droplets can be detected after incorporation in the gel. Channels are formed by the organic solvent (oil), which are coated by surfactant molecules and a water layer in which the enzyme can be hosted.
AB - (Hydroxypropyl)methyl cellulose (HPMC) can be used to form gels integrating a w/o microemulsion. The formulation in which a microemulsion is mixed with a hydrated HPMC matrix has been successfully used as a carrier of biocompatible ingredients. However, little is known about the structure of these systems. To elucidate this, scanning electron microscopy was used to examine the morphology and the bulk of the microemulsion-based gels (MBGs) and small-angle X-ray scattering to clarify the structure and detect any residual reverse micelles after microemulsion incorporation in the gel. Electron paramagnetic resonance spectroscopy was applied using spin probes to investigate the polar and non-polar areas of the gel. Furthermore, the enzyme-labelling technique was followed to investigate the location of an enzyme in the matrix. A structural model for HPMC matrix is proposed according to which, although a w/o microemulsion is essential to form the final gel, no microemulsion droplets can be detected after incorporation in the gel. Channels are formed by the organic solvent (oil), which are coated by surfactant molecules and a water layer in which the enzyme can be hosted.
KW - (hydroxypropyl)methyl cellulose (HPMC)
KW - Electronparamagnetic resonance (EPR)
KW - Lipase
KW - Scanningelectronmicroscopy(SEM)
KW - Small angle X-rayscattering(SAXS)
UR - http://www.scopus.com/inward/record.url?scp=85095742666&partnerID=8YFLogxK
U2 - 10.3390/nano10112204
DO - 10.3390/nano10112204
M3 - Article
AN - SCOPUS:85095742666
SN - 2079-4991
VL - 10
SP - 1
EP - 20
JO - Nanomaterials
JF - Nanomaterials
IS - 11
M1 - 2204
ER -