Abstract
Within the last two decades, the methylotrophic yeast Pichia pastoris (Komagataella phaffii) has become an important alternative to E. coli or mammalian cell lines for the production of recombinant proteins. Easy handling, strong promoters, and high cell density cultivations as well as the capability of posttranslational modifications are some of the major benefits of this yeast. The high secretion capacity and low level of endogenously secreted proteins further promoted the rapid development of a versatile Pichia pastoris toolbox. This chapter reviews common and new “Pichia tools” and their specific features. Special focus is given to expression strains, such as different methanol utilization, protease-deficient or glycoengineered strains, combined with application highlights. Different promoters and signal sequences are also discussed.
| Originalsprache | englisch |
|---|---|
| Titel | Yeast Metabolic Engineering: Methods and Protocols |
| Redakteure/-innen | Valeria Mapelli, Maurizio Bettiga |
| Erscheinungsort | New York, U.S.A. |
| Herausgeber (Verlag) | Springer |
| Kapitel | 6 |
| Seiten | 79-112 |
| Seitenumfang | 34 |
| Auflage | 2nd |
| ISBN (elektronisch) | 978-1-0716-2399-2 |
| ISBN (Print) | 978-1-0716-2398-5 |
| DOIs | |
| Publikationsstatus | Veröffentlicht - 1 Juni 2022 |
Publikationsreihe
| Name | Methods in Molecular Biology |
|---|---|
| Herausgeber (Verlag) | Humana Press |
| Band | 2513 |
| ISSN (Print) | 1064-3745 |
| ISSN (elektronisch) | 1940-6029 |
ASJC Scopus subject areas
- Genetik
- Molekularbiologie
Fields of Expertise
- Human- & Biotechnology
Treatment code (Nähere Zuordnung)
- Basic - Fundamental (Grundlagenforschung)
- Popular Scientific
- Review
UN SDGs
Dieser Output leistet einen Beitrag zu folgendem(n) Ziel(en) für nachhaltige Entwicklung
