Stabilization of supramolecular membrane protein–lipid bilayer assemblies through immobilization in a crystalline exoskeleton

Fabian C. Herbert, Sameera S. Abeyrathna, Nisansala S. Abeyrathna, Yalini H. Wijesundara, Olivia R. Brohlin, Francesco Carraro, Heinz Amenitsch, Paolo Falcaro, Michael A. Luzuriaga, Alejandra Durand-Silva, Shashini D. Diwakara, Ronald A. Smaldone, Gabriele Meloni*, Jeremiah J. Gassensmith

*Korrespondierende/r Autor/in für diese Arbeit

Publikation: Beitrag in einer FachzeitschriftArtikel

Abstract

Artificial native-like lipid bilayer systems constructed from phospholipids assembling into unilamellar liposomes allow the reconstitution of detergent-solubilized transmembrane proteins into supramolecular lipid-protein assemblies called proteoliposomes, which mimic cellular membranes. Stabilization of these complexes remains challenging because of their chemical composition, the hydrophobicity and structural instability of membrane proteins, and the lability of interactions between protein, detergent, and lipids within micelles and lipid bilayers. In this work we demonstrate that metastable lipid, protein-detergent, and protein-lipid supramolecular complexes can be successfully generated and immobilized within zeolitic-imidazole framework (ZIF) to enhance their stability against chemical and physical stressors. Upon immobilization in ZIF bio-composites, blank liposomes, and model transmembrane metal transporters in detergent micelles or embedded in proteoliposomes resist elevated temperatures, exposure to chemical denaturants, aging, and mechanical stresses. Extensive morphological and functional characterization of the assemblies upon exfoliation reveal that all these complexes encapsulated within the framework maintain their native morphology, structure, and activity, which is otherwise lost rapidly without immobilization.

Originalspracheenglisch
Aufsatznummer2202
FachzeitschriftNature Communications
Jahrgang12
Ausgabenummer1
DOIs
PublikationsstatusVeröffentlicht - Dez 2021

ASJC Scopus subject areas

  • !!Chemistry(all)
  • !!Biochemistry, Genetics and Molecular Biology(all)
  • !!Physics and Astronomy(all)

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