Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents

Bianca Grabner, Nazario, Maria T. Gundersen, Sandrine Lois, Sébastian Fantini, Sebastian Bartsch, John M. Woodley, Heidrun Gruber-Wölfler

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co lyophilization, and showed increased stability in all of the five tested organic solvents. Co-lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8 fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5’ phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5 bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.
Originalspracheenglisch
Seiten (von - bis)11-19
FachzeitschriftMolecular Catalysis
Jahrgang452
DOIs
PublikationsstatusVeröffentlicht - Jun 2018

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Ionic Liquids
Transaminases
Ionic liquids
solid phases
colloiding
coatings
Coatings
room temperature
liquids
Temperature
Pyridoxal Phosphate
Cyclohexane
cyclohexane
Organic solvents
Reaction rates
enzymes
phosphates
Phosphates
reaction kinetics
Enzymes

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Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents. / Grabner, Bianca; Nazario; Gundersen, Maria T.; Lois, Sandrine; Fantini, Sébastian; Bartsch, Sebastian; Woodley, John M.; Gruber-Wölfler, Heidrun.

in: Molecular Catalysis, Jahrgang 452, 06.2018, S. 11-19.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Grabner, Bianca ; Nazario ; Gundersen, Maria T. ; Lois, Sandrine ; Fantini, Sébastian ; Bartsch, Sebastian ; Woodley, John M. ; Gruber-Wölfler, Heidrun. / Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents. in: Molecular Catalysis. 2018 ; Jahrgang 452. S. 11-19.
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abstract = "ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co lyophilization, and showed increased stability in all of the five tested organic solvents. Co-lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8 fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5’ phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5 bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.",
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T1 - Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents

AU - Grabner, Bianca

AU - Nazario,

AU - Gundersen, Maria T.

AU - Lois, Sandrine

AU - Fantini, Sébastian

AU - Bartsch, Sebastian

AU - Woodley, John M.

AU - Gruber-Wölfler, Heidrun

PY - 2018/6

Y1 - 2018/6

N2 - ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co lyophilization, and showed increased stability in all of the five tested organic solvents. Co-lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8 fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5’ phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5 bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.

AB - ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co lyophilization, and showed increased stability in all of the five tested organic solvents. Co-lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8 fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5’ phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5 bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.

U2 - 10.1016/j.mcat.2018.03.012

DO - 10.1016/j.mcat.2018.03.012

M3 - Article

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EP - 19

JO - Molecular Catalysis

JF - Molecular Catalysis

SN - 2468-8231

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