Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents

Bianca Grabner; Nazario; Maria T. Gundersen; Sandrine Lois; Sébastian Fantini; Sebastian Bartsch; John M. Woodley; Heidrun Gruber-Wölfler

doi:10.1016/j.mcat.2018.03.012

Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents

Bianca Grabner, Nazario, Maria T. Gundersen, Sandrine Lois, Sébastian Fantini, Sebastian Bartsch, John M. Woodley, Heidrun Gruber-Wölfler

Institut für Prozess- und Partikeltechnik (6690)

Publikation: Beitrag in einer Fachzeitschrift › Artikel › Begutachtung

Abstract

ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co lyophilization, and showed increased stability in all of the five tested organic solvents. Co-lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8 fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5’ phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5 bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.

Originalsprache	englisch
Seiten (von - bis)	11-19
Fachzeitschrift	Molecular Catalysis
Jahrgang	452
DOIs	https://doi.org/10.1016/j.mcat.2018.03.012
Publikationsstatus	Veröffentlicht - Juni 2018

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10.1016/j.mcat.2018.03.012

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@article{a49450e6d3f9453198bd45099e9da609,

title = "Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents",

abstract = "ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co lyophilization, and showed increased stability in all of the five tested organic solvents. Co-lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8 fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5{\textquoteright} phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5 bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.",

author = "Bianca Grabner and Nazario and Gundersen, {Maria T.} and Sandrine Lois and S{\'e}bastian Fantini and Sebastian Bartsch and Woodley, {John M.} and Heidrun Gruber-W{\"o}lfler",

year = "2018",

month = jun,

doi = "10.1016/j.mcat.2018.03.012",

language = "English",

volume = "452",

pages = "11--19",

journal = "Molecular Catalysis",

issn = "2468-8231",

publisher = "Elsevier B.V.",

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TY - JOUR

T1 - Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents

AU - Grabner, Bianca

AU - Nazario,

AU - Gundersen, Maria T.

AU - Lois, Sandrine

AU - Fantini, Sébastian

AU - Bartsch, Sebastian

AU - Woodley, John M.

AU - Gruber-Wölfler, Heidrun

PY - 2018/6

Y1 - 2018/6

N2 - ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co lyophilization, and showed increased stability in all of the five tested organic solvents. Co-lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8 fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5’ phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5 bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.

AB - ω-Transaminases ATA-40, ATA-47 and ATA-82P were coated with room-temperature solid phase ionic liquids (RTSPILs) by means of three methods, melt coating, precipitation coating, and co lyophilization, and showed increased stability in all of the five tested organic solvents. Co-lyophilization and melt coating were further found to have an activating effect on the enzymes. The former led to an up to 8 fold increase of reaction rate and excellent recyclability. The coating also protected the cofactor pyridoxal 5’ phosphate (PLP), which is essential for transaminase activity, from degradation, leading to a reaction proceeding for 27 days. With this method the sparingly water soluble substrate 5 bromo-1-indenone could be processed enzymatically in cyclohexane as solvent.

U2 - 10.1016/j.mcat.2018.03.012

DO - 10.1016/j.mcat.2018.03.012

M3 - Article

SN - 2468-8231

VL - 452

SP - 11

EP - 19

JO - Molecular Catalysis

JF - Molecular Catalysis

ER -

Room-temperature solid phase ionic liquid (RTSPIL) coated omega-transaminase: Development and application in orgranic solvents

Abstract

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