Recombinant lipoxygenases (LOX) and hydroperoxide lyases (HPL) for the synthesis of green leaf volatiles

Publikation: Beitrag in einer FachzeitschriftReview eines Fachbereichs (Review article)ForschungBegutachtung

Abstract

Green leaf volatiles (GLVs) are C6- or C9-aldehydes, -alcohols and -esters which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focusses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids like linoleic and α-linolenic acid. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production.
Originalspracheenglisch
Seitenumfang59
FachzeitschriftJournal of agricultural and food chemistry
DOIs
PublikationsstatusAngenommen/In Druck - 8 Okt 2019

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Lipoxygenases
lyases
hydroperoxides
lipoxygenase
synthesis
Enzymes
enzymes
Aldehydes
aldehydes
leaves
fatty acids
Keto Acids
Enzyme Stability
Smell
peroxidation
industrial applications
Plant Extracts
smell
biotic stress
Poaceae

Fields of Expertise

  • Human- & Biotechnology

Dies zitieren

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title = "Recombinant lipoxygenases (LOX) and hydroperoxide lyases (HPL) for the synthesis of green leaf volatiles",
abstract = "Green leaf volatiles (GLVs) are C6- or C9-aldehydes, -alcohols and -esters which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focusses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids like linoleic and α-linolenic acid. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production.",
author = "Holly Stolterfoht and Claudia Rinnofner and Margit Winkler and Harald Pichler",
year = "2019",
month = "10",
day = "8",
doi = "10.1021/acs.jafc.9b02690",
language = "English",
journal = "Journal of agricultural and food chemistry",
issn = "0021-8561",
publisher = "American Chemical Society",

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TY - JOUR

T1 - Recombinant lipoxygenases (LOX) and hydroperoxide lyases (HPL) for the synthesis of green leaf volatiles

AU - Stolterfoht, Holly

AU - Rinnofner, Claudia

AU - Winkler, Margit

AU - Pichler, Harald

PY - 2019/10/8

Y1 - 2019/10/8

N2 - Green leaf volatiles (GLVs) are C6- or C9-aldehydes, -alcohols and -esters which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focusses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids like linoleic and α-linolenic acid. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production.

AB - Green leaf volatiles (GLVs) are C6- or C9-aldehydes, -alcohols and -esters which are released by plants in response to biotic or abiotic stresses. These compounds are named for their characteristic smell reminiscent of freshly mowed grass. This review focusses on GLVs and the two major pathway enzymes responsible for their formation: lipoxygenases (LOXs) and fatty acid hydroperoxide lyases (HPLs). LOXs catalyze the peroxidation of unsaturated fatty acids like linoleic and α-linolenic acid. Hydroperoxy fatty acids are further converted by HPLs into aldehydes and oxo-acids. In many industrial applications plant extracts have been used as LOX and HPL sources. However, these processes are limited by low enzyme concentration, stability and specificity. Alternatively, recombinant enzymes can be used as biocatalysts for GLV synthesis. The increasing number of well-characterized enzymes efficiently expressed by microbial hosts will foster the development of innovative biocatalytic processes for GLV production.

U2 - 10.1021/acs.jafc.9b02690

DO - 10.1021/acs.jafc.9b02690

M3 - Review article

JO - Journal of agricultural and food chemistry

JF - Journal of agricultural and food chemistry

SN - 0021-8561

ER -