Practical Considerions Regarding the Choice of High-Throughput-Assays

Robert Kourist, Carolin Mügge

Publikation: Beitrag in Buch/Bericht/KonferenzbandBeitrag in Buch/BerichtForschungBegutachtung

Abstract

Since the first successful directed evolution studies in the 1990s, experience shows that the success of protein engineering for an enzyme of interest mainly depends on the extent of knowledge of its structure-function relationships and the ability to characterize a sufficiently large number of enzyme variants.1 A good understanding of a catalytic property makes it possible to achieve an objective with very few amino acid exchanges. In contrast, a powerful assay makes it possible to extend the number of investigated variants and thus to increase the likelihood to find an improved mutant.
Originalspracheenglisch
TitelProtein Engineering- Methods and Protocols
Redakteure/-innenUwe Bornscheuer, Matthias Höhne
Seiten189-208
Seitenumfang18
Band1685
PublikationsstatusVeröffentlicht - 1 Sep 2017

Publikationsreihe

NameSpringer Protocols Methods in Molecular Biology
Herausgeber (Verlag)Humana Press

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Assays
Throughput
Enzymes
Amino Acids
Proteins

Fields of Expertise

  • Human- & Biotechnology

Dies zitieren

Kourist, R., & Mügge, C. (2017). Practical Considerions Regarding the Choice of High-Throughput-Assays. in U. Bornscheuer, & M. Höhne (Hrsg.), Protein Engineering- Methods and Protocols (Band 1685, S. 189-208). (Springer Protocols Methods in Molecular Biology).

Practical Considerions Regarding the Choice of High-Throughput-Assays. / Kourist, Robert; Mügge, Carolin.

Protein Engineering- Methods and Protocols. Hrsg. / Uwe Bornscheuer; Matthias Höhne. Band 1685 2017. S. 189-208 (Springer Protocols Methods in Molecular Biology).

Publikation: Beitrag in Buch/Bericht/KonferenzbandBeitrag in Buch/BerichtForschungBegutachtung

Kourist, R & Mügge, C 2017, Practical Considerions Regarding the Choice of High-Throughput-Assays. in U Bornscheuer & M Höhne (Hrsg.), Protein Engineering- Methods and Protocols. Bd. 1685, Springer Protocols Methods in Molecular Biology, S. 189-208.
Kourist R, Mügge C. Practical Considerions Regarding the Choice of High-Throughput-Assays. in Bornscheuer U, Höhne M, Hrsg., Protein Engineering- Methods and Protocols. Band 1685. 2017. S. 189-208. (Springer Protocols Methods in Molecular Biology).
Kourist, Robert ; Mügge, Carolin. / Practical Considerions Regarding the Choice of High-Throughput-Assays. Protein Engineering- Methods and Protocols. Hrsg. / Uwe Bornscheuer ; Matthias Höhne. Band 1685 2017. S. 189-208 (Springer Protocols Methods in Molecular Biology).
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