Optical Ammonia Sensor for Continuous Bioprocess Monitoring

Maximilian Maierhofer; Sergey Borisov; Torsten Mayr

Optical Ammonia Sensor for Continuous Bioprocess Monitoring

Maximilian Maierhofer, Sergey Borisov, Torsten Mayr

Institut für Analytische Chemie und Lebensmittelchemie (6450)

Publikation: Beitrag in Buch/Bericht/Konferenzband › Beitrag in einem Konferenzband › Begutachtung

Abstract

We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.

Originalsprache	englisch
Titel	Proceedings Eurosensors 2018
Redakteure/-innen	Anton Köck, Marco Deluca
Herausgeber (Verlag)	MDPI AG
Seitenumfang	4
Band	2
Publikationsstatus	Veröffentlicht - 19 Nov. 2018
Veranstaltung	Eurosensors 2018 - Karl Franzens Universität Graz, Graz, Österreich Dauer: 9 Sept. 2018 → 12 Sept. 2018 https://www.eurosensors2018.eu

Konferenz

Konferenz	Eurosensors 2018
Land/Gebiet	Österreich
Ort	Graz
Zeitraum	9/09/18 → 12/09/18
Internetadresse	https://www.eurosensors2018.eu

Schlagwörter

Optischer Sensor
Ammoniak
Bioprozessüberwachung
Fluoreszenz

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@inproceedings{1509ae404cd54a98a0679b563add6f38,

title = "Optical Ammonia Sensor for Continuous Bioprocess Monitoring",

abstract = "We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material. ",

keywords = "Optischer Sensor, Ammoniak, Bioprozess{\"u}berwachung, Fluoreszenz, Ammonia, optical sensor, process monitoring, fluorescent dye",

author = "Maximilian Maierhofer and Sergey Borisov and Torsten Mayr",

year = "2018",

month = nov,

day = "19",

language = "English",

volume = "2",

editor = "Anton K{\"o}ck and Marco Deluca",

booktitle = "Proceedings Eurosensors 2018",

publisher = "MDPI AG",

address = "Switzerland",

note = "Eurosensors 2018 ; Conference date: 09-09-2018 Through 12-09-2018",

url = "https://www.eurosensors2018.eu",

}

TY - GEN

T1 - Optical Ammonia Sensor for Continuous Bioprocess Monitoring

AU - Maierhofer, Maximilian

AU - Borisov, Sergey

AU - Mayr, Torsten

PY - 2018/11/19

Y1 - 2018/11/19

N2 - We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.

AB - We present an optical ammonia sensor suitable for bioprocess monitoring. A fluorescent dye is physically entrapped in a polyurethane hydrogel forming an emulsion system with vinylterminated polydimethylsiloxane (PDMS). The sensing layer is covered by a hydrophobic porous membrane which excludes hydrophilic substances. Ammonia, diffuses through this barrier and PDMS to the protonated dye, whereby it deprotonates the dye and switches off its emission. Readout is performed with a miniaturized phase fluorimeter combined with optical fibers. Dual-lifetime referencing (DLR) acts as detection method and Egyptian blue as reference material.

KW - Optischer Sensor

KW - Ammoniak

KW - Bioprozessüberwachung

KW - Fluoreszenz

KW - Ammonia

KW - optical sensor

KW - process monitoring

KW - fluorescent dye

M3 - Conference paper

VL - 2

BT - Proceedings Eurosensors 2018

A2 - Köck, Anton

A2 - Deluca, Marco

PB - MDPI AG

T2 - Eurosensors 2018

Y2 - 9 September 2018 through 12 September 2018

ER -

Optical Ammonia Sensor for Continuous Bioprocess Monitoring

Abstract

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Schlagwörter

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