@article{b31998a34d454c909028779f7c944ae1,
title = "One, two, many: Strategies to alter the number of carbohydrate binding sites of lectins",
abstract = "Carbohydrates are more than an energy-storage. They are ubiquitously found on cells and most proteins, where they encode biological information. Lectins bind these carbohydrates and are essential for translating the encoded information into biological functions and processes. Hundreds of lectins are known, and they are found in all domains of life. For half a century, researchers have been preparing variants of lectins in which the binding sites are varied. In this way, the traits of the lectins such as the affinity, avidity and specificity towards their ligands as well as their biological efficacy were changed. These efforts helped to unravel the biological importance of lectins and resulted in improved variants for biotechnological exploitation and potential medical applications. This review gives an overview on the methods for the preparation of artificial lectins and complexes thereof and how reducing or increasing the number of binding sites affects their function.",
keywords = "Artificial lectin multimers, Lectin engineering, Lectins, Neolectins, Synthetic biology",
author = "Felix Tobola and Birgit Wiltschi",
note = "Funding Information: This work was supported by the Austrian Science Fund (FWF): I 1708-B22 (ERA-SynBio) as well as by FWF project no. W901 (DK Molecular Enzymology). The COMET center: acib: Next Generation Bioproduction is funded by BMK, BMDW, SFG, Standortagentur Tirol, Government of Lower Austria und Vienna Business Agency in the framework of COMET - Competence Centers for Excellent Technologies. The COMET-Funding Program is managed by the Austrian Research Promotion Agency FFG. B.W. acknowledges support by BioTechMed-Graz. Funding Information: This work was supported by the Austrian Science Fund (FWF): I 1708-B22 (ERA-SynBio) as well as by FWF project no. W901 (DK Molecular Enzymology). The COMET center: acib: Next Generation Bioproduction is funded by BMK , BMDW , SFG , Standortagentur Tirol , Government of Lower Austria und Vienna Business Agency in the framework of COMET - Competence Centers for Excellent Technologies . The COMET-Funding Program is managed by the Austrian Research Promotion Agency FFG . B.W. acknowledges support by BioTechMed-Graz. Publisher Copyright: {\textcopyright} 2022 The Authors",
year = "2022",
month = nov,
doi = "10.1016/j.biotechadv.2022.108020",
language = "English",
volume = "60",
journal = "Biotechnology Advances",
issn = "0734-9750",
publisher = "Elsevier Inc.",
}