TY - JOUR
T1 - Novel protein-repellent and antimicrobial polysaccharide multilayer thin films
AU - Korica, Matea
AU - Fras Zemljič, Lidija
AU - Bračič, Matej
AU - Kargl, Rupert
AU - Spirk, Stefan
AU - Reishofer, David
AU - Mihajlovski, Katarina
AU - Kostić, Mirjana
PY - 2019/1/1
Y1 - 2019/1/1
N2 - Nanostructured and bio-active polysaccharide-based thin films were manufactured by means of subsequent spin-coated deposition of a regenerated cellulose (RC) layer and a 2,2,6,6-Tetramethylpiperidine-1-oxyl radical (TEMPO) oxidised cellulose nanofibril (TOCN) layer. The bio-activity of the bilayer was achieved by addition of chitosan (CS). The chitosan was either mixed with the TOCN (TOCN+CS) and deposited on the RC layer by spin-coating, or deposited on the RC and TOCN bilayer by pumping its aqueous solution with various pH over the surface of the bilayer. The water content of the thin films and the CS interactions with the bilayer during deposition were studied in situ by means of a quartz crystal microbalance with dissipation (QCM-D). The pH dependent charging behaviour of the TOCN, TOCN+CS and CS dispersions was evaluated by pH-potentiometric titrations. The surface morphology of the thin films was characterised by atomic force microscopy (AFM). The bio-activity of the thin films was evaluated by studying their protein-repellent properties in situ with a continuous flow of bovine serum albumin (BSA) by means of QCM-D and by evaluating their antibacterial properties in vitro against Staphylococcus aureus and Escherichia coli. These polysaccharide-based thin films are high value-added products because of their multifunctionality, high water absorbance capacity, protein-repellence and antimicrobial activity, and have the potential for medical application as a wound dressing material.
AB - Nanostructured and bio-active polysaccharide-based thin films were manufactured by means of subsequent spin-coated deposition of a regenerated cellulose (RC) layer and a 2,2,6,6-Tetramethylpiperidine-1-oxyl radical (TEMPO) oxidised cellulose nanofibril (TOCN) layer. The bio-activity of the bilayer was achieved by addition of chitosan (CS). The chitosan was either mixed with the TOCN (TOCN+CS) and deposited on the RC layer by spin-coating, or deposited on the RC and TOCN bilayer by pumping its aqueous solution with various pH over the surface of the bilayer. The water content of the thin films and the CS interactions with the bilayer during deposition were studied in situ by means of a quartz crystal microbalance with dissipation (QCM-D). The pH dependent charging behaviour of the TOCN, TOCN+CS and CS dispersions was evaluated by pH-potentiometric titrations. The surface morphology of the thin films was characterised by atomic force microscopy (AFM). The bio-activity of the thin films was evaluated by studying their protein-repellent properties in situ with a continuous flow of bovine serum albumin (BSA) by means of QCM-D and by evaluating their antibacterial properties in vitro against Staphylococcus aureus and Escherichia coli. These polysaccharide-based thin films are high value-added products because of their multifunctionality, high water absorbance capacity, protein-repellence and antimicrobial activity, and have the potential for medical application as a wound dressing material.
KW - antimicrobial properties
KW - chitosan
KW - nanostructured polysaccharide thin films
KW - protein-repellent properties
KW - TEMPO oxidised cellulose nanofibrils
UR - http://www.scopus.com/inward/record.url?scp=85055534517&partnerID=8YFLogxK
U2 - 10.1515/hf-2018-0094
DO - 10.1515/hf-2018-0094
M3 - Article
AN - SCOPUS:85055534517
SN - 0018-3830
VL - 73
SP - 93
EP - 103
JO - Holzforschung
JF - Holzforschung
IS - 1
ER -