N-acetylaspartate catabolism determines cytosolic acetyl-CoA levels and histone acetylation in brown adipocytes.

Andreas Prokesch, Helmut Josef Pelzmann, Ariane Pessentheiner, Katharina Huber, Corina Madreiter-Sokolowski, Anne Drougard, Matthias Schittmayer, Dagmar Kolb, Christoph Magnes, Gert Trausinger, Wolfgang Graier, Ruth Birner-Gruenberger, J Andrew Pospisilik, Juliane Gertrude Bogner-Strauß

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Histone acetylation depends on the abundance of nucleo-cytoplasmic acetyl-CoA. Here, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. N-acetylaspartate (NAA) is a highly abundant brain metabolite catabolized by aspartoacylase yielding aspartate and acetate. The latter can be further used for acetyl-CoA production. Prior to this work, the presence of NAA has not been described in adipocytes. Here, we show that accumulation of NAA decreases the brown adipocyte phenotype. We increased intracellular NAA concentrations in brown adipocytes via media supplementation or knock-down of aspartoacylase and measured reduced lipolysis, thermogenic gene expression, and oxygen consumption. Combinations of approaches to increase intracellular NAA levels showed additive effects on lipolysis and gene repression, nearly abolishing the expression of Ucp1, Cidea, Prdm16, and Ppara. Transcriptome analyses of aspartoacylase knock-down cells indicate deficiencies in acetyl-CoA and lipid metabolism. Concordantly, cytoplasmic acetyl-CoA levels and global histone H3 acetylation were decreased. Further, activating histone marks (H3K27ac and H3K9ac) in promoters/enhancers of brown marker genes showed reduced acetylation status. Taken together, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. Thereby, we mechanistically connect the NAA pathway to the epigenomic regulation of gene expression, modulating the phenotype of brown adipocytes.
Originalspracheenglisch
Seitenumfang12
FachzeitschriftScientific reports
Ausgabenummer6
PublikationsstatusVeröffentlicht - 5 Apr 2016

Fingerprint

Brown Adipocytes
Acetyl Coenzyme A
Acetylation
Histones
Lipolysis
Histone Code
Phenotype
Gene Expression Regulation
Gene Expression Profiling
Lipid Metabolism
Adipocytes
Epigenomics
Aspartic Acid
Oxygen Consumption
Genes
N-acetylaspartate
Acetates
Gene Expression
Brain
aspartoacylase

Schlagwörter

    Dies zitieren

    N-acetylaspartate catabolism determines cytosolic acetyl-CoA levels and histone acetylation in brown adipocytes. / Prokesch, Andreas; Pelzmann, Helmut Josef; Pessentheiner, Ariane; Huber, Katharina; Madreiter-Sokolowski, Corina; Drougard, Anne; Schittmayer, Matthias; Kolb, Dagmar; Magnes, Christoph; Trausinger, Gert; Graier, Wolfgang; Birner-Gruenberger, Ruth; Pospisilik, J Andrew; Bogner-Strauß, Juliane Gertrude.

    in: Scientific reports, Nr. 6, 05.04.2016.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    Prokesch, A, Pelzmann, HJ, Pessentheiner, A, Huber, K, Madreiter-Sokolowski, C, Drougard, A, Schittmayer, M, Kolb, D, Magnes, C, Trausinger, G, Graier, W, Birner-Gruenberger, R, Pospisilik, JA & Bogner-Strauß, JG 2016, 'N-acetylaspartate catabolism determines cytosolic acetyl-CoA levels and histone acetylation in brown adipocytes.' Scientific reports, Nr. 6.
    Prokesch A, Pelzmann HJ, Pessentheiner A, Huber K, Madreiter-Sokolowski C, Drougard A et al. N-acetylaspartate catabolism determines cytosolic acetyl-CoA levels and histone acetylation in brown adipocytes. Scientific reports. 2016 Apr 5;(6).
    Prokesch, Andreas ; Pelzmann, Helmut Josef ; Pessentheiner, Ariane ; Huber, Katharina ; Madreiter-Sokolowski, Corina ; Drougard, Anne ; Schittmayer, Matthias ; Kolb, Dagmar ; Magnes, Christoph ; Trausinger, Gert ; Graier, Wolfgang ; Birner-Gruenberger, Ruth ; Pospisilik, J Andrew ; Bogner-Strauß, Juliane Gertrude. / N-acetylaspartate catabolism determines cytosolic acetyl-CoA levels and histone acetylation in brown adipocytes. in: Scientific reports. 2016 ; Nr. 6.
    @article{99f81a58cb91419fa80c26cf3782f716,
    title = "N-acetylaspartate catabolism determines cytosolic acetyl-CoA levels and histone acetylation in brown adipocytes.",
    abstract = "Histone acetylation depends on the abundance of nucleo-cytoplasmic acetyl-CoA. Here, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. N-acetylaspartate (NAA) is a highly abundant brain metabolite catabolized by aspartoacylase yielding aspartate and acetate. The latter can be further used for acetyl-CoA production. Prior to this work, the presence of NAA has not been described in adipocytes. Here, we show that accumulation of NAA decreases the brown adipocyte phenotype. We increased intracellular NAA concentrations in brown adipocytes via media supplementation or knock-down of aspartoacylase and measured reduced lipolysis, thermogenic gene expression, and oxygen consumption. Combinations of approaches to increase intracellular NAA levels showed additive effects on lipolysis and gene repression, nearly abolishing the expression of Ucp1, Cidea, Prdm16, and Ppara. Transcriptome analyses of aspartoacylase knock-down cells indicate deficiencies in acetyl-CoA and lipid metabolism. Concordantly, cytoplasmic acetyl-CoA levels and global histone H3 acetylation were decreased. Further, activating histone marks (H3K27ac and H3K9ac) in promoters/enhancers of brown marker genes showed reduced acetylation status. Taken together, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. Thereby, we mechanistically connect the NAA pathway to the epigenomic regulation of gene expression, modulating the phenotype of brown adipocytes.",
    keywords = "N-acetylaspartate, Aspartoacylase, acetyl-CoA, metabolism, epigenetics",
    author = "Andreas Prokesch and Pelzmann, {Helmut Josef} and Ariane Pessentheiner and Katharina Huber and Corina Madreiter-Sokolowski and Anne Drougard and Matthias Schittmayer and Dagmar Kolb and Christoph Magnes and Gert Trausinger and Wolfgang Graier and Ruth Birner-Gruenberger and Pospisilik, {J Andrew} and Bogner-Strau{\ss}, {Juliane Gertrude}",
    year = "2016",
    month = "4",
    day = "5",
    language = "English",
    journal = "Scientific reports",
    issn = "2045-2322",
    publisher = "Nature Publishing Group",
    number = "6",

    }

    TY - JOUR

    T1 - N-acetylaspartate catabolism determines cytosolic acetyl-CoA levels and histone acetylation in brown adipocytes.

    AU - Prokesch, Andreas

    AU - Pelzmann, Helmut Josef

    AU - Pessentheiner, Ariane

    AU - Huber, Katharina

    AU - Madreiter-Sokolowski, Corina

    AU - Drougard, Anne

    AU - Schittmayer, Matthias

    AU - Kolb, Dagmar

    AU - Magnes, Christoph

    AU - Trausinger, Gert

    AU - Graier, Wolfgang

    AU - Birner-Gruenberger, Ruth

    AU - Pospisilik, J Andrew

    AU - Bogner-Strauß, Juliane Gertrude

    PY - 2016/4/5

    Y1 - 2016/4/5

    N2 - Histone acetylation depends on the abundance of nucleo-cytoplasmic acetyl-CoA. Here, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. N-acetylaspartate (NAA) is a highly abundant brain metabolite catabolized by aspartoacylase yielding aspartate and acetate. The latter can be further used for acetyl-CoA production. Prior to this work, the presence of NAA has not been described in adipocytes. Here, we show that accumulation of NAA decreases the brown adipocyte phenotype. We increased intracellular NAA concentrations in brown adipocytes via media supplementation or knock-down of aspartoacylase and measured reduced lipolysis, thermogenic gene expression, and oxygen consumption. Combinations of approaches to increase intracellular NAA levels showed additive effects on lipolysis and gene repression, nearly abolishing the expression of Ucp1, Cidea, Prdm16, and Ppara. Transcriptome analyses of aspartoacylase knock-down cells indicate deficiencies in acetyl-CoA and lipid metabolism. Concordantly, cytoplasmic acetyl-CoA levels and global histone H3 acetylation were decreased. Further, activating histone marks (H3K27ac and H3K9ac) in promoters/enhancers of brown marker genes showed reduced acetylation status. Taken together, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. Thereby, we mechanistically connect the NAA pathway to the epigenomic regulation of gene expression, modulating the phenotype of brown adipocytes.

    AB - Histone acetylation depends on the abundance of nucleo-cytoplasmic acetyl-CoA. Here, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. N-acetylaspartate (NAA) is a highly abundant brain metabolite catabolized by aspartoacylase yielding aspartate and acetate. The latter can be further used for acetyl-CoA production. Prior to this work, the presence of NAA has not been described in adipocytes. Here, we show that accumulation of NAA decreases the brown adipocyte phenotype. We increased intracellular NAA concentrations in brown adipocytes via media supplementation or knock-down of aspartoacylase and measured reduced lipolysis, thermogenic gene expression, and oxygen consumption. Combinations of approaches to increase intracellular NAA levels showed additive effects on lipolysis and gene repression, nearly abolishing the expression of Ucp1, Cidea, Prdm16, and Ppara. Transcriptome analyses of aspartoacylase knock-down cells indicate deficiencies in acetyl-CoA and lipid metabolism. Concordantly, cytoplasmic acetyl-CoA levels and global histone H3 acetylation were decreased. Further, activating histone marks (H3K27ac and H3K9ac) in promoters/enhancers of brown marker genes showed reduced acetylation status. Taken together, we present a novel route for cytoplasmic acetyl-CoA production in brown adipocytes. Thereby, we mechanistically connect the NAA pathway to the epigenomic regulation of gene expression, modulating the phenotype of brown adipocytes.

    KW - N-acetylaspartate

    KW - Aspartoacylase

    KW - acetyl-CoA

    KW - metabolism

    KW - epigenetics

    M3 - Article

    JO - Scientific reports

    JF - Scientific reports

    SN - 2045-2322

    IS - 6

    ER -