Myeloperoxidase-responsive materials for infection detection based on immobilized aminomethoxyphenol

Doris Schiffer, Gregor Tegl, Robert Vielnascher, Hansjörg Weber, Alexandra Herrero-Rollett, Eva Maria Sigl, Andrea Heinzle, Georg M. Guebitz

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

There is a strong need for simple and fast diagnostic tools for the detection of wound infection. Immune system-derived enzymes like myeloperoxidase are efficient biomarkers for wound infection that emerge in the early stage infection process. In this study, 5-amino-2-methoxyphenol was functionalized with alkoxysilane to allow visual detection of MPO on carrier materials, for example, in test strips. Indeed, MPO activity was visually detectable in short time in wound background. Oxidation of the substrate was followed spectrophotometrically and proved via HPLC. LC-ESI TOF and NMR analyses unveiled the reaction mechanism and a dimeric reaction product responsible for the visualization of MPO activity. The substrate specificity and sensitivity toward MPO detection was proved and tests with infected wound fluids were successfully performed. The study demonstrates the suitability of the novel MPO substrate for the detection of wound infection and the covalent immobilization on diagnostic carrier materials.

Originalspracheenglisch
Seiten (von - bis)2553-2560
FachzeitschriftBiotechnology and Bioengineering
Jahrgang113
Ausgabenummer12
DOIs
PublikationsstatusVeröffentlicht - 2016

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Wound Infection
Peroxidase
Substrates
Infection
Guaiacol
Immune system
Wounds and Injuries
Biomarkers
Substrate Specificity
Reaction products
Immobilization
Immune System
Visualization
Enzymes
High Pressure Liquid Chromatography
Nuclear magnetic resonance
Oxidation
Fluids

Schlagwörter

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • !!Applied Microbiology and Biotechnology

    Dies zitieren

    Schiffer, D., Tegl, G., Vielnascher, R., Weber, H., Herrero-Rollett, A., Sigl, E. M., ... Guebitz, G. M. (2016). Myeloperoxidase-responsive materials for infection detection based on immobilized aminomethoxyphenol. Biotechnology and Bioengineering, 113(12), 2553-2560. https://doi.org/10.1002/bit.26025

    Myeloperoxidase-responsive materials for infection detection based on immobilized aminomethoxyphenol. / Schiffer, Doris; Tegl, Gregor; Vielnascher, Robert; Weber, Hansjörg; Herrero-Rollett, Alexandra; Sigl, Eva Maria; Heinzle, Andrea; Guebitz, Georg M.

    in: Biotechnology and Bioengineering, Jahrgang 113, Nr. 12, 2016, S. 2553-2560.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    Schiffer, D, Tegl, G, Vielnascher, R, Weber, H, Herrero-Rollett, A, Sigl, EM, Heinzle, A & Guebitz, GM 2016, 'Myeloperoxidase-responsive materials for infection detection based on immobilized aminomethoxyphenol' Biotechnology and Bioengineering, Jg. 113, Nr. 12, S. 2553-2560. https://doi.org/10.1002/bit.26025
    Schiffer, Doris ; Tegl, Gregor ; Vielnascher, Robert ; Weber, Hansjörg ; Herrero-Rollett, Alexandra ; Sigl, Eva Maria ; Heinzle, Andrea ; Guebitz, Georg M. / Myeloperoxidase-responsive materials for infection detection based on immobilized aminomethoxyphenol. in: Biotechnology and Bioengineering. 2016 ; Jahrgang 113, Nr. 12. S. 2553-2560.
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