TY - CHAP
T1 - Multi-technique analysis of extracellular vesicles: not only size matters
AU - Perissinotto, Fabio
AU - Senigagliesi, B.
AU - Vaccari, Lisa
AU - Pachetti, Maria
AU - D'Amico, Francesco
AU - Amenitsch, Heinz
AU - Sartori, Barbara
AU - Pachler, K.
AU - Mayr, M.
AU - Gimona, M.
AU - Rohde, E
AU - Caponnetto, F.
AU - Cesselli, D.
AU - Casalis, L.
AU - Parisse, P.
PY - 2020/7/9
Y1 - 2020/7/9
N2 - Extracellular vesicles (EVs) are small vesicles ensuring transport of molecules between cells and throughout the body. Their small size and biological and physical functions make them optimal candidates as therapeutic agents in several medical frameworks. The elucidation of biophysical and biochemical characteristics of EVs is crucial for the understanding of their interaction with recipient cells and their functional activity. In particular, for therapeutic applications understanding how the process of manufacturing affects the biological function of EVs is mandatory before going to the clinical testing. The absence of standardized methodologies and technologies to establish reliable criteria has been the main hurdle for real therapeutic applications of EVs. Here we exploited a combination of different biophysical techniques (Fourier Transform Infrared Spectroscopy, Ultraviolet Resonant Raman Spectroscopy, Atomic Force Microscopy and Small Angle X-Ray Scattering) to screen EVs derived from umbilical cord-mesenchymal stem cells with different isolation procedures, evidencing subtle differences in the biophysical properties of EVs. The results underline the need of a multiparametric analysis to address size, stability and purity of different preparations and to correlate phenotypic parameters of EVs with their functional activity.
AB - Extracellular vesicles (EVs) are small vesicles ensuring transport of molecules between cells and throughout the body. Their small size and biological and physical functions make them optimal candidates as therapeutic agents in several medical frameworks. The elucidation of biophysical and biochemical characteristics of EVs is crucial for the understanding of their interaction with recipient cells and their functional activity. In particular, for therapeutic applications understanding how the process of manufacturing affects the biological function of EVs is mandatory before going to the clinical testing. The absence of standardized methodologies and technologies to establish reliable criteria has been the main hurdle for real therapeutic applications of EVs. Here we exploited a combination of different biophysical techniques (Fourier Transform Infrared Spectroscopy, Ultraviolet Resonant Raman Spectroscopy, Atomic Force Microscopy and Small Angle X-Ray Scattering) to screen EVs derived from umbilical cord-mesenchymal stem cells with different isolation procedures, evidencing subtle differences in the biophysical properties of EVs. The results underline the need of a multiparametric analysis to address size, stability and purity of different preparations and to correlate phenotypic parameters of EVs with their functional activity.
KW - Atomic force microscopy
KW - Fourier transform infrared spectroscopy
KW - extracellular vescicles
KW - Small angle X-ray scattering
KW - ultraviolet resonant raman spectroscopy
KW - Attenuated total reflectance- fourier transform infrared spectroscopy
KW - Ultraviolet resonant Raman spectroscopy
KW - Extracellular vesicles
KW - Small angle X-Ray scattering
UR - http://www.scopus.com/inward/record.url?scp=85087769257&partnerID=8YFLogxK
U2 - 10.1016/bs.abl.2020.05.004
DO - 10.1016/bs.abl.2020.05.004
M3 - Chapter
SN - 9780128209684
VL - 32
T3 - Advances in Biomembranes and Lipid Self-Assembly
SP - 157
EP - 177
BT - Advances in Biomembranes and Lipid Self-Assembly
A2 - Bongiovanni, Antonella
A2 - Pocsfalvi, Gabriella
A2 - Manno, Mauro
A2 - Kralj-Iglič, Veronika
A2 - Kralj-Iglič, Veronika
PB - Elsevier B.V.
ER -