In this work, a test strip design is developed for a single-use potassium-sensor for use in the patients´ homes. The test strip shall draw a single drop of blood from the fingertip, separate the erythrocytes from the blood and guide the gained plasma via a microfluidic channel towards the measuring electrodes. These steps shall be performed without the occurrence of hemolysis of the sample. Since blood is used for the measurement, all materials that get in contact with the sample have to meet the requirement of haemocompatibility. The timeframe between applying the drop of blood onto the test strip and to entirely covering the measurement electrodes with the separated blood plasma should not exceed 2 minutes. The materials used for the microfluidic components were investigated on hydrophilic wettability (contact angle), haemocompatibility (measurement of the level of hemolysis), ability of plasma separation and passive channel suction effect (based on capillary forces). The final design allows plasma separation from a 10 µl blood drop and delivering a sufficient volume of separated plasma for measuring the potassium concentration in less than 16 seconds with a plasma purity of 100%. The level of hemolysis remains unchanged as the used materials do not cause additional hemolysis. The plasma recovery rate was 55%. Thus, it meets the requirements of hemolysis-free separation of plasma, as well as the timing.
|Titel in Übersetzung||Microfluidic design on single test strips for a potassium home monitoring system|
|Qualifikation||Master of Science|
|Betreuer/-in / Berater/-in|
|Publikationsstatus||Veröffentlicht - 2016|