Leloir Glycosyltransferases as Biocatalysts for Chemical Production

Bernd Nidetzky, Alexander Gutmann, Chao Zhong

Publikation: Beitrag in einer FachzeitschriftReview eines Fachbereichs (Review article)ForschungBegutachtung

Abstract

Glycosylation is a chemical transformation that is centrally important in all glycoscience and related technologies. Catalysts offering good control over reactivity and selectivity in synthetic glycosylations are much sought. The enzymes responsible for glycosylations in natural biosynthesis are sugar-nucleotide-dependent (Leloir) glycosyltransferases. Discovery-oriented synthesis and pilot batch production of oligosaccharides and glycosylated natural products have previously relied on Leloir glycosyltransferases. However, despite their perceived synthetic utility, Leloir glycosyltransferases are yet to see widespread application in industrial biocatalysis. Here we show progress and limitations in the development of Leloir glycosyltransferases into robust biocatalytic systems for use in glycosylations for chemical production. Obtaining highly active and stable (whole-cell) catalysts that can promote the desired glycosylation(s) coupled to an in situ sugar nucleotide supply remains a difficult problem. Optimizing glycosyltransferase cascade reactions for high process efficiency is another. Glycosylations of some natural products (e.g., flavonoids, terpenoids) involve acceptor substrate solubility as a special challenge for biocatalytic process design. Strategies to overcome these problems are illustrated from examples of integrated biocatalytic process development with this class of enzymes.

Originalspracheenglisch
Seiten (von - bis)6283-6300
Seitenumfang18
FachzeitschriftACS Catalysis
Jahrgang8
Ausgabenummer7
DOIs
PublikationsstatusVeröffentlicht - 6 Jul 2018

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Glycosylation
Glycosyltransferases
Biocatalysts
Enzymes
Nucleotides
Biological Products
Sugars
Flavonoids
Oligosaccharides
Catalysts
Catalyst selectivity
Biosynthesis
Terpenes
Process design
Solubility
Substrates

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    ASJC Scopus subject areas

    • !!Catalysis

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    Leloir Glycosyltransferases as Biocatalysts for Chemical Production. / Nidetzky, Bernd; Gutmann, Alexander; Zhong, Chao.

    in: ACS Catalysis, Jahrgang 8, Nr. 7, 06.07.2018, S. 6283-6300.

    Publikation: Beitrag in einer FachzeitschriftReview eines Fachbereichs (Review article)ForschungBegutachtung

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