Abstract
Lacto-N-biose 1,2-oxazoline was prepared chemo-enzymatically and shown to be a donor substrate for β-1,3-glycosylation of lactose by the wild-type and glycosynthase variants (D320E, D320A, Y419F) of Bifidobacterium bifidum β-N-hexosaminidase. Lacto-N-tetraose, a core structure of human milk oligosaccharides, was formed in 20-60% yield of donor substrate (up to 8 mM product titre), depending on the degree of selectivity control by the enzyme used.
Originalsprache | englisch |
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Seiten (von - bis) | 5661-5665 |
Seitenumfang | 5 |
Fachzeitschrift | Organic and Biomolecular Chemistry |
Jahrgang | 17 |
Ausgabenummer | 23 |
DOIs | |
Publikationsstatus | Veröffentlicht - 1 Jan. 2019 |
ASJC Scopus subject areas
- Biochemie
- Physikalische und Theoretische Chemie
- Organische Chemie