TY - JOUR
T1 - Kinetic study of activation and deactivation of adsorbed cellulase during enzymatic conversion of alkaline peroxide oxidation-pretreated corn cob to sugar
AU - Ayeni, Augustine Omoniyi
AU - Agboola, Oluranti
AU - Daramola, Michael Olawale
AU - Grabner, Bianca
AU - Oni, Babalola Aisosa
AU - Babatunde, Damilola Elizabeth
AU - Evwodere, Joseph
N1 - Publisher Copyright:
© 2021, The Korean Institute of Chemical Engineers.
PY - 2021/1
Y1 - 2021/1
N2 - Corn cob lignocellulosic biomass is one of the useful precursors for the alternative production of fuels and chemicals. Understanding the kinetics of enzymatic conversion of corn cob through kinetic models could provide indepth knowledge and increase the predictive ability for process design and optimization. In this study, models based on the semi-mechanistic rate equations, first-order decay exponential function of time for adsorbed enzymes, structural and diffusion coefficient for adsorption were used to estimate kinetic parameters for the enzymatic conversion of alkaline peroxide oxidation (APO) pretreated corn cob to sugar. Fitting a first-order inactivation model of adsorbed cellulases to account for experimental hydrolysis data, the apparent hydrolysis rate constant (k2=29.51 min−1), the inactivation rate constant (k3=0.269 min−1), and reactivation rate constant (k4=0.0048 min−1) were estimated. Regressed values of apparent maximum rate, Vmax, app, for adsorbed enzymes reduced appreciably with time to more than 98% at 96 h. The diffusion limit model showed that the diffusion resistance increased with increasing enzyme concentrations.
AB - Corn cob lignocellulosic biomass is one of the useful precursors for the alternative production of fuels and chemicals. Understanding the kinetics of enzymatic conversion of corn cob through kinetic models could provide indepth knowledge and increase the predictive ability for process design and optimization. In this study, models based on the semi-mechanistic rate equations, first-order decay exponential function of time for adsorbed enzymes, structural and diffusion coefficient for adsorption were used to estimate kinetic parameters for the enzymatic conversion of alkaline peroxide oxidation (APO) pretreated corn cob to sugar. Fitting a first-order inactivation model of adsorbed cellulases to account for experimental hydrolysis data, the apparent hydrolysis rate constant (k2=29.51 min−1), the inactivation rate constant (k3=0.269 min−1), and reactivation rate constant (k4=0.0048 min−1) were estimated. Regressed values of apparent maximum rate, Vmax, app, for adsorbed enzymes reduced appreciably with time to more than 98% at 96 h. The diffusion limit model showed that the diffusion resistance increased with increasing enzyme concentrations.
KW - Adsorption
KW - Enzymatic Hydrolysis
KW - Inactivation
KW - Kinetic Models
KW - Lignocellulose
UR - http://www.scopus.com/inward/record.url?scp=85098748138&partnerID=8YFLogxK
U2 - 10.1007/s11814-020-0667-2
DO - 10.1007/s11814-020-0667-2
M3 - Article
AN - SCOPUS:85098748138
SN - 0256-1115
VL - 38
SP - 81
EP - 89
JO - Korean Journal of Chemical Engineering
JF - Korean Journal of Chemical Engineering
IS - 1
ER -