Kinetic insights into ϵ-caprolactone synthesis: Improvement of an enzymatic cascade reaction

Christian Scherkus, Sandy Schmidt, Uwe T. Bornscheuer, Harald Gröger, Selin Kara, Andreas Liese*

*Korrespondierende/r Autor/in für diese Arbeit

Publikation: Beitrag in einer FachzeitschriftArtikel

Abstract

A computational approach for the simulation and prediction of a linear three-step enzymatic cascade for the synthesis of ϵ-caprolactone (ECL) coupling an alcohol dehydrogenase (ADH), a cyclohexanone monooxygenase (CHMO), and a lipase for the subsequent hydrolysis of ECL to 6-hydroxyhexanoic acid (6-HHA). A kinetic model was developed with an accuracy of prediction for a fed-batch mode of 37% for substrate cyclohexanol (CHL), 90% for ECL, and >99% for the final product 6-HHA. Due to a severe inhibition of the CHMO by CHL, a batch synthesis was shown to be less efficient than a fed-batch approach. In the fed-batch synthesis, full conversion of 100 mM CHL was 28% faster with an analytical yield of 98% compared to 49% in case of the batch synthesis. The lipase-catalyzed hydrolysis of ECL to 6-HHA circumvents the inhibition of the CHMO by ECL enabling a 24% higher product concentration of 6-HHA compared to ECL in case of the fed-batch synthesis without lipase. Biotechnol. Bioeng. 2017;114: 1215–1221.

Originalspracheenglisch
Seiten (von - bis)1215-1221
Seitenumfang7
FachzeitschriftBiotechnology and Bioengineering
Jahrgang114
Ausgabenummer6
DOIs
PublikationsstatusVeröffentlicht - 1 Jun 2017
Extern publiziertJa

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • !!Applied Microbiology and Biotechnology

Fingerprint Untersuchen Sie die Forschungsthemen von „Kinetic insights into ϵ-caprolactone synthesis: Improvement of an enzymatic cascade reaction“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren