TY - JOUR
T1 - Involvement of a putative substrate binding site in the biogenesis and assembly of phosphatidylserine decarboxylase 1 from Saccharomyces cerevisiae
AU - Di Bartolomeo, Francesca
AU - Doan, Kim Nguyen
AU - Athenstaedt, Karin
AU - Becker, Thomas
AU - Daum, Günther
PY - 2017/7/1
Y1 - 2017/7/1
N2 - In the yeast Saccharomyces cerevisiae, the mitochondrial phosphatidylserine decarboxylase 1 (Psd1p) produces the largest amount of cellular phosphatidylethanolamine (PE). Psd1p is synthesized as a larger precursor on cytosolic ribosomes and then imported into mitochondria in a three-step processing event leading to the formation of an α-subunit and a β-subunit. The α-subunit harbors a highly conserved motif, which was proposed to be involved in phosphatidylserine (PS) binding. Here, we present a molecular analysis of this consensus motif for the function of Psd1p by using Psd1p variants bearing either deletions or point mutations in this region. Our data show that mutations in this motif affect processing and stability of Psd1p, and consequently the enzyme's activity. Thus, we conclude that this consensus motif is essential for structural integrity and processing of Psd1p.
AB - In the yeast Saccharomyces cerevisiae, the mitochondrial phosphatidylserine decarboxylase 1 (Psd1p) produces the largest amount of cellular phosphatidylethanolamine (PE). Psd1p is synthesized as a larger precursor on cytosolic ribosomes and then imported into mitochondria in a three-step processing event leading to the formation of an α-subunit and a β-subunit. The α-subunit harbors a highly conserved motif, which was proposed to be involved in phosphatidylserine (PS) binding. Here, we present a molecular analysis of this consensus motif for the function of Psd1p by using Psd1p variants bearing either deletions or point mutations in this region. Our data show that mutations in this motif affect processing and stability of Psd1p, and consequently the enzyme's activity. Thus, we conclude that this consensus motif is essential for structural integrity and processing of Psd1p.
KW - Mitochondria
KW - Phosphatidylethanolamine
KW - Phosphatidylserine decarboxylase 1
KW - Phospholipid
KW - Saccharomyces cerevisiae
UR - http://www.scopus.com/inward/record.url?scp=85019037324&partnerID=8YFLogxK
U2 - 10.1016/j.bbalip.2017.04.007
DO - 10.1016/j.bbalip.2017.04.007
M3 - Article
C2 - 28473294
AN - SCOPUS:85019037324
SN - 1388-1981
VL - 1862
SP - 716
EP - 725
JO - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
JF - Biochimica et Biophysica Acta - Molecular and Cell Biology of Lipids
IS - 7
ER -