Investigation of luminescent dye-doped or rare-earth-doped monodisperse silica nanospheres for DNA microarray labelling

F. Enrichi, R. Riccò, A. Meneghello, R. Pierobon, E. Cretaio, F. Marinello, P. Schiavuta, A. Parma, P. Riello, A. Benedetti

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

DNA microarray is a high-throughput technology used for detection and quantification of nucleic acid molecules and others of biological interest. The analysis is resulting by specific hybridization between probe sequences deposited in array and a target ss-DNA usually functionalized by a luminescent dye. These organic labels have well known disadvantages like photobleaching and limited sensitivity. Therefore in this work we investigate a different strategy, based on the use of inorganic silica nanospheres incorporating standard luminescent dyes or rare earth ions. The synthesis and characterization of these biomarkers is reported and their application to the DNA microarray technology in comparison to the use of standard molecular fluorophores or commercial quantum dots is discussed. We show that dye doped silica spheres provides a significant increase of the optical emission signal with respect to the use of free dyes, while rare earth doped silica spheres allow reducing or completely avoiding the background noise. These aspects, together with their cheap and easy synthesis, stability in water, easy surface functionalization and bio-compatibility makes them very promising for present and future applications in bio-labelling and bio-imaging.

Originalspracheenglisch
Seiten (von - bis)1652-1658
Seitenumfang7
FachzeitschriftOptical materials
Jahrgang32
Ausgabenummer12
DOIs
PublikationsstatusVeröffentlicht - Okt 2010
Extern publiziertJa

Fingerprint

Nanospheres
Microarrays
Silicon Dioxide
Labeling
Rare earths
marking
DNA
Coloring Agents
rare earth elements
Dyes
deoxyribonucleic acid
dyes
Silica
silicon dioxide
Photobleaching
Fluorophores
biomarkers
Nucleic acids
background noise
nucleic acids

Schlagwörter

    ASJC Scopus subject areas

    • !!Electronic, Optical and Magnetic Materials
    • !!Electrical and Electronic Engineering
    • !!Atomic and Molecular Physics, and Optics
    • !!Computer Science(all)

    Dies zitieren

    Investigation of luminescent dye-doped or rare-earth-doped monodisperse silica nanospheres for DNA microarray labelling. / Enrichi, F.; Riccò, R.; Meneghello, A.; Pierobon, R.; Cretaio, E.; Marinello, F.; Schiavuta, P.; Parma, A.; Riello, P.; Benedetti, A.

    in: Optical materials, Jahrgang 32, Nr. 12, 10.2010, S. 1652-1658.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    Enrichi, F, Riccò, R, Meneghello, A, Pierobon, R, Cretaio, E, Marinello, F, Schiavuta, P, Parma, A, Riello, P & Benedetti, A 2010, 'Investigation of luminescent dye-doped or rare-earth-doped monodisperse silica nanospheres for DNA microarray labelling' Optical materials, Jg. 32, Nr. 12, S. 1652-1658. https://doi.org/10.1016/j.optmat.2010.04.026
    Enrichi, F. ; Riccò, R. ; Meneghello, A. ; Pierobon, R. ; Cretaio, E. ; Marinello, F. ; Schiavuta, P. ; Parma, A. ; Riello, P. ; Benedetti, A. / Investigation of luminescent dye-doped or rare-earth-doped monodisperse silica nanospheres for DNA microarray labelling. in: Optical materials. 2010 ; Jahrgang 32, Nr. 12. S. 1652-1658.
    @article{771909ea2a77497d8bf87699135a3cf7,
    title = "Investigation of luminescent dye-doped or rare-earth-doped monodisperse silica nanospheres for DNA microarray labelling",
    abstract = "DNA microarray is a high-throughput technology used for detection and quantification of nucleic acid molecules and others of biological interest. The analysis is resulting by specific hybridization between probe sequences deposited in array and a target ss-DNA usually functionalized by a luminescent dye. These organic labels have well known disadvantages like photobleaching and limited sensitivity. Therefore in this work we investigate a different strategy, based on the use of inorganic silica nanospheres incorporating standard luminescent dyes or rare earth ions. The synthesis and characterization of these biomarkers is reported and their application to the DNA microarray technology in comparison to the use of standard molecular fluorophores or commercial quantum dots is discussed. We show that dye doped silica spheres provides a significant increase of the optical emission signal with respect to the use of free dyes, while rare earth doped silica spheres allow reducing or completely avoiding the background noise. These aspects, together with their cheap and easy synthesis, stability in water, easy surface functionalization and bio-compatibility makes them very promising for present and future applications in bio-labelling and bio-imaging.",
    keywords = "DNA microarray, Luminescence, Silica nanoparticles",
    author = "F. Enrichi and R. Ricc{\`o} and A. Meneghello and R. Pierobon and E. Cretaio and F. Marinello and P. Schiavuta and A. Parma and P. Riello and A. Benedetti",
    year = "2010",
    month = "10",
    doi = "10.1016/j.optmat.2010.04.026",
    language = "English",
    volume = "32",
    pages = "1652--1658",
    journal = "Optical materials",
    issn = "0925-3467",
    publisher = "Elsevier B.V.",
    number = "12",

    }

    TY - JOUR

    T1 - Investigation of luminescent dye-doped or rare-earth-doped monodisperse silica nanospheres for DNA microarray labelling

    AU - Enrichi, F.

    AU - Riccò, R.

    AU - Meneghello, A.

    AU - Pierobon, R.

    AU - Cretaio, E.

    AU - Marinello, F.

    AU - Schiavuta, P.

    AU - Parma, A.

    AU - Riello, P.

    AU - Benedetti, A.

    PY - 2010/10

    Y1 - 2010/10

    N2 - DNA microarray is a high-throughput technology used for detection and quantification of nucleic acid molecules and others of biological interest. The analysis is resulting by specific hybridization between probe sequences deposited in array and a target ss-DNA usually functionalized by a luminescent dye. These organic labels have well known disadvantages like photobleaching and limited sensitivity. Therefore in this work we investigate a different strategy, based on the use of inorganic silica nanospheres incorporating standard luminescent dyes or rare earth ions. The synthesis and characterization of these biomarkers is reported and their application to the DNA microarray technology in comparison to the use of standard molecular fluorophores or commercial quantum dots is discussed. We show that dye doped silica spheres provides a significant increase of the optical emission signal with respect to the use of free dyes, while rare earth doped silica spheres allow reducing or completely avoiding the background noise. These aspects, together with their cheap and easy synthesis, stability in water, easy surface functionalization and bio-compatibility makes them very promising for present and future applications in bio-labelling and bio-imaging.

    AB - DNA microarray is a high-throughput technology used for detection and quantification of nucleic acid molecules and others of biological interest. The analysis is resulting by specific hybridization between probe sequences deposited in array and a target ss-DNA usually functionalized by a luminescent dye. These organic labels have well known disadvantages like photobleaching and limited sensitivity. Therefore in this work we investigate a different strategy, based on the use of inorganic silica nanospheres incorporating standard luminescent dyes or rare earth ions. The synthesis and characterization of these biomarkers is reported and their application to the DNA microarray technology in comparison to the use of standard molecular fluorophores or commercial quantum dots is discussed. We show that dye doped silica spheres provides a significant increase of the optical emission signal with respect to the use of free dyes, while rare earth doped silica spheres allow reducing or completely avoiding the background noise. These aspects, together with their cheap and easy synthesis, stability in water, easy surface functionalization and bio-compatibility makes them very promising for present and future applications in bio-labelling and bio-imaging.

    KW - DNA microarray

    KW - Luminescence

    KW - Silica nanoparticles

    UR - http://www.scopus.com/inward/record.url?scp=77957285605&partnerID=8YFLogxK

    U2 - 10.1016/j.optmat.2010.04.026

    DO - 10.1016/j.optmat.2010.04.026

    M3 - Article

    VL - 32

    SP - 1652

    EP - 1658

    JO - Optical materials

    JF - Optical materials

    SN - 0925-3467

    IS - 12

    ER -