Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli

Jihye Jung, Jan Braun, Tibor Czabany, Bernd Nidetzky

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Enzymatic transformations of the nitrile group are important in biology as well as in synthetic chemistry. The enzyme QueF catalyses the conversion of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1), a unique approach towards biological four-electron reduction of a nitrile to an amine. The catalytic reaction involves a QueF–preQ0 thioimidate adduct that is converted to preQ1 in two NADPH dependent reduction steps via an imine intermediate. The QueF active site comprises a cysteine nucleophile flanked by an aspartic acid and additionally contains a histidine. Here, we used mutagenesis of E. coli QueF (C190A, C190S, D197A, D197H, and H229A) to study the functional interplay between these enzyme residues in covalent catalysis. Substitution of Cys190 or Asp197 annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity. The H229A variant readily forms the thioimidate adduct and is 24-fold less active for preQ0 reduction than wild-type ecQueF (kcat = 7.2 min−1). Using isothermal titration calorimetry, we show that the non-covalent step of preQ0 binding involves proton uptake mediated by Asp197 with His229 as the likely protonated group. Catalytic proton transfer from the Cys190 thiol via Asp197 to the nitrile nitrogen promotes the covalent intermediate. We suggest that protonated (charged) His229 facilitates the polarization of the substrate nitrile for nucleophilic attack on carbon by Cys190, and through proton relay via Asp197, it could provide the proton for re-protonating Cys190 during the formation of the imine intermediate.
Originalspracheenglisch
Seiten (von - bis)842-853
Seitenumfang12
FachzeitschriftCatalysis Science & Technology
Jahrgang9
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - 14 Jan 2019

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Nitriles
Proton transfer
Catalysis
Escherichia coli
Protons
Oxidoreductases
Amines
Enzymes
Imines
Mutagenesis
Nucleophiles
Calorimetry
Titration
Substitution reactions
Polarization
Nitrogen
Carbon
NADP
Histidine
Sulfhydryl Compounds

ASJC Scopus subject areas

  • !!Biochemistry
  • !!Molecular Biology
  • !!Structural Biology

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Interplay of nucleophilic catalysis with proton transfer in the nitrile reductase QueF from Escherichia coli. / Jung, Jihye; Braun, Jan; Czabany, Tibor; Nidetzky, Bernd.

in: Catalysis Science & Technology, Jahrgang 9, Nr. 3, 14.01.2019, S. 842-853.

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

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abstract = "Enzymatic transformations of the nitrile group are important in biology as well as in synthetic chemistry. The enzyme QueF catalyses the conversion of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1), a unique approach towards biological four-electron reduction of a nitrile to an amine. The catalytic reaction involves a QueF–preQ0 thioimidate adduct that is converted to preQ1 in two NADPH dependent reduction steps via an imine intermediate. The QueF active site comprises a cysteine nucleophile flanked by an aspartic acid and additionally contains a histidine. Here, we used mutagenesis of E. coli QueF (C190A, C190S, D197A, D197H, and H229A) to study the functional interplay between these enzyme residues in covalent catalysis. Substitution of Cys190 or Asp197 annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity. The H229A variant readily forms the thioimidate adduct and is 24-fold less active for preQ0 reduction than wild-type ecQueF (kcat = 7.2 min−1). Using isothermal titration calorimetry, we show that the non-covalent step of preQ0 binding involves proton uptake mediated by Asp197 with His229 as the likely protonated group. Catalytic proton transfer from the Cys190 thiol via Asp197 to the nitrile nitrogen promotes the covalent intermediate. We suggest that protonated (charged) His229 facilitates the polarization of the substrate nitrile for nucleophilic attack on carbon by Cys190, and through proton relay via Asp197, it could provide the proton for re-protonating Cys190 during the formation of the imine intermediate.",
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AU - Braun, Jan

AU - Czabany, Tibor

AU - Nidetzky, Bernd

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AB - Enzymatic transformations of the nitrile group are important in biology as well as in synthetic chemistry. The enzyme QueF catalyses the conversion of 7-cyano-7-deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1), a unique approach towards biological four-electron reduction of a nitrile to an amine. The catalytic reaction involves a QueF–preQ0 thioimidate adduct that is converted to preQ1 in two NADPH dependent reduction steps via an imine intermediate. The QueF active site comprises a cysteine nucleophile flanked by an aspartic acid and additionally contains a histidine. Here, we used mutagenesis of E. coli QueF (C190A, C190S, D197A, D197H, and H229A) to study the functional interplay between these enzyme residues in covalent catalysis. Substitution of Cys190 or Asp197 annihilates preQ0 covalent binding and largely disrupts the nitrile-to-amine reductase activity. The H229A variant readily forms the thioimidate adduct and is 24-fold less active for preQ0 reduction than wild-type ecQueF (kcat = 7.2 min−1). Using isothermal titration calorimetry, we show that the non-covalent step of preQ0 binding involves proton uptake mediated by Asp197 with His229 as the likely protonated group. Catalytic proton transfer from the Cys190 thiol via Asp197 to the nitrile nitrogen promotes the covalent intermediate. We suggest that protonated (charged) His229 facilitates the polarization of the substrate nitrile for nucleophilic attack on carbon by Cys190, and through proton relay via Asp197, it could provide the proton for re-protonating Cys190 during the formation of the imine intermediate.

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