Integrated process development of a reactive extraction concept for itaconic acid and application to a real fermentation broth

Jannick Gorden, Elena Geiser, Nick Wierckx, Lars M. Blank, Tim Zeiner, Christoph Brandenbusch

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Itaconic acid (IA) has a high potential to be used as a bio-based platform chemical and its biocatalytic production via fermentation has significantly improved within the last decade. Additionally downstream processing using reactive extraction (RE) was described, potentially enabling a more efficient sustainable bioprocess producing IA. The bottleneck to overcome is the connection of up- and downstream processing, caused by lack of biocompatibility of the RE systems and direct application to fermentation broth. Within this study, a biocompatible RE system for IA is defined (pH dependency, extraction mechanism) and used for direct application to a fermentation broth. By optimizing the biocatalyst, the production medium, and the extraction system in an integrated approach, it was possible to define critical parameters that enabled a tuning of the overall bioprocess. With an extraction yield of YIA = 0.80 ± 0.03, IA could be produced as sole carboxylic acid (b IA ,0 aq = 0.490 mol/kgaq) using a RE system consisting of ethyl oleate as organic solvent and tri-n-octylamine as extractant (bT-C8 org = 0.6 mol/kgorg). This work is a proof of concept and demonstrates that by joint consideration of up- and downstream processing, optimized bioprocesses can be developed.

Originalspracheenglisch
FachzeitschriftEngineering in life sciences
DOIs
PublikationsstatusVeröffentlicht - 28 Feb 2017

Fingerprint

Fermentation
Acids
Processing
Carboxylic Acids
Biocatalysts
Joints
itaconic acid
Carboxylic acids
Biocompatibility
Organic solvents
Tuning
Enzymes

Schlagwörter

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • Environmental engineering

    Dies zitieren

    Integrated process development of a reactive extraction concept for itaconic acid and application to a real fermentation broth. / Gorden, Jannick; Geiser, Elena; Wierckx, Nick; Blank, Lars M.; Zeiner, Tim; Brandenbusch, Christoph.

    in: Engineering in life sciences, 28.02.2017.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    @article{da2a000a166d473c9752dff35bd1f3bd,
    title = "Integrated process development of a reactive extraction concept for itaconic acid and application to a real fermentation broth",
    abstract = "Itaconic acid (IA) has a high potential to be used as a bio-based platform chemical and its biocatalytic production via fermentation has significantly improved within the last decade. Additionally downstream processing using reactive extraction (RE) was described, potentially enabling a more efficient sustainable bioprocess producing IA. The bottleneck to overcome is the connection of up- and downstream processing, caused by lack of biocompatibility of the RE systems and direct application to fermentation broth. Within this study, a biocompatible RE system for IA is defined (pH dependency, extraction mechanism) and used for direct application to a fermentation broth. By optimizing the biocatalyst, the production medium, and the extraction system in an integrated approach, it was possible to define critical parameters that enabled a tuning of the overall bioprocess. With an extraction yield of YIA = 0.80 ± 0.03, IA could be produced as sole carboxylic acid (b IA ,0 aq = 0.490 mol/kgaq) using a RE system consisting of ethyl oleate as organic solvent and tri-n-octylamine as extractant (bT-C8 org = 0.6 mol/kgorg). This work is a proof of concept and demonstrates that by joint consideration of up- and downstream processing, optimized bioprocesses can be developed.",
    keywords = "Biocatalysis, Downstream processing, Itaconic acid, Reactive extraction, Ustilago maydis",
    author = "Jannick Gorden and Elena Geiser and Nick Wierckx and Blank, {Lars M.} and Tim Zeiner and Christoph Brandenbusch",
    year = "2017",
    month = "2",
    day = "28",
    doi = "10.1002/elsc.201600253",
    language = "English",
    journal = "Engineering in life sciences",
    issn = "1618-0240",
    publisher = "Wiley-VCH",

    }

    TY - JOUR

    T1 - Integrated process development of a reactive extraction concept for itaconic acid and application to a real fermentation broth

    AU - Gorden, Jannick

    AU - Geiser, Elena

    AU - Wierckx, Nick

    AU - Blank, Lars M.

    AU - Zeiner, Tim

    AU - Brandenbusch, Christoph

    PY - 2017/2/28

    Y1 - 2017/2/28

    N2 - Itaconic acid (IA) has a high potential to be used as a bio-based platform chemical and its biocatalytic production via fermentation has significantly improved within the last decade. Additionally downstream processing using reactive extraction (RE) was described, potentially enabling a more efficient sustainable bioprocess producing IA. The bottleneck to overcome is the connection of up- and downstream processing, caused by lack of biocompatibility of the RE systems and direct application to fermentation broth. Within this study, a biocompatible RE system for IA is defined (pH dependency, extraction mechanism) and used for direct application to a fermentation broth. By optimizing the biocatalyst, the production medium, and the extraction system in an integrated approach, it was possible to define critical parameters that enabled a tuning of the overall bioprocess. With an extraction yield of YIA = 0.80 ± 0.03, IA could be produced as sole carboxylic acid (b IA ,0 aq = 0.490 mol/kgaq) using a RE system consisting of ethyl oleate as organic solvent and tri-n-octylamine as extractant (bT-C8 org = 0.6 mol/kgorg). This work is a proof of concept and demonstrates that by joint consideration of up- and downstream processing, optimized bioprocesses can be developed.

    AB - Itaconic acid (IA) has a high potential to be used as a bio-based platform chemical and its biocatalytic production via fermentation has significantly improved within the last decade. Additionally downstream processing using reactive extraction (RE) was described, potentially enabling a more efficient sustainable bioprocess producing IA. The bottleneck to overcome is the connection of up- and downstream processing, caused by lack of biocompatibility of the RE systems and direct application to fermentation broth. Within this study, a biocompatible RE system for IA is defined (pH dependency, extraction mechanism) and used for direct application to a fermentation broth. By optimizing the biocatalyst, the production medium, and the extraction system in an integrated approach, it was possible to define critical parameters that enabled a tuning of the overall bioprocess. With an extraction yield of YIA = 0.80 ± 0.03, IA could be produced as sole carboxylic acid (b IA ,0 aq = 0.490 mol/kgaq) using a RE system consisting of ethyl oleate as organic solvent and tri-n-octylamine as extractant (bT-C8 org = 0.6 mol/kgorg). This work is a proof of concept and demonstrates that by joint consideration of up- and downstream processing, optimized bioprocesses can be developed.

    KW - Biocatalysis

    KW - Downstream processing

    KW - Itaconic acid

    KW - Reactive extraction

    KW - Ustilago maydis

    UR - http://www.scopus.com/inward/record.url?scp=85016280083&partnerID=8YFLogxK

    U2 - 10.1002/elsc.201600253

    DO - 10.1002/elsc.201600253

    M3 - Article

    JO - Engineering in life sciences

    JF - Engineering in life sciences

    SN - 1618-0240

    ER -