Influence of the relative humidity on aminosilane molecular grafting properties

Fabio Lisi, Davide Carta, Laura Villanova, Irene Poli, Dario Buso, Stefano Costacurta, Anita J. Hill, Paolo Falcaro

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Functional coatings with amino groups are used in a wide range of biochemistry-related applications. A technological platform that takes advantage of the affinity between amino-functionalized coatings and biomolecules is the DNA microarray methodology. Reliability and reproducibility of the microarray data strongly depend on the quality of the substrate; therefore, a proper awareness of how the storage conditions affect the amino-functionalized coatings is necessary. In this work we have studied the influence of different relative humidity levels on aminomethyl- silane coatings prepared via sol-gel methodology. Drops of a buffer solution containing a luminescent dye have been deposited (or spotted) on the coating; the dye molecules react with the amino-groups and leave circular luminescent marks (spots) on the substrate surface. Shape and luminescence of the spots, as well as background signals, have been monitored using a microarray laser scanner. With the proposed protocol we have measured the changes of these variables due to different storage conditions. FT-IR measurements have been performed to investigate the related chemistry changes.

Originalspracheenglisch
Seiten (von - bis)246-253
Seitenumfang8
FachzeitschriftJournal of sol gel science and technology
Jahrgang60
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - Dez 2011
Extern publiziertJa

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    ASJC Scopus subject areas

    • !!Chemistry(all)
    • !!Condensed Matter Physics
    • !!Biomaterials
    • !!Ceramics and Composites
    • !!Electronic, Optical and Magnetic Materials
    • !!Materials Chemistry

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