TY - JOUR
T1 - Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts
T2 - Current state and future prospects
AU - Raschmanová, Hana
AU - Weninger, Astrid
AU - Glieder, Anton
AU - Kovar, Karin
AU - Vogl, Thomas
PY - 2018/5/1
Y1 - 2018/5/1
N2 - Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.
AB - Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.
KW - Candida albicans/glabrata
KW - CRISPR-Cas
KW - Expression optimization
KW - Metabolic engineering
KW - Pichia pastoris
KW - Saccharomyces cerevisiae
KW - Schizosaccharomyces pombe
KW - Synthetic biology
KW - Yarrowia lipolytica
KW - Yeasts
UR - http://www.scopus.com/inward/record.url?scp=85040622209&partnerID=8YFLogxK
U2 - 10.1016/j.biotechadv.2018.01.006
DO - 10.1016/j.biotechadv.2018.01.006
M3 - Review article
C2 - 29331410
AN - SCOPUS:85040622209
SN - 0734-9750
VL - 36
SP - 641
EP - 665
JO - Biotechnology Advances
JF - Biotechnology Advances
IS - 3
ER -