Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

Hana Raschmanová, Astrid Weninger, Anton Glieder, Karin Kovar, Thomas Vogl*

*Korrespondierende/r Autor/-in für diese Arbeit

Publikation: Beitrag in einer FachzeitschriftReview eines Fachbereichs (Review article)Begutachtung

Abstract

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.

Originalspracheenglisch
Seiten (von - bis)641-665
Seitenumfang25
FachzeitschriftBiotechnology Advances
Jahrgang36
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - 1 Mai 2018

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Angewandte Mikrobiologie und Biotechnologie

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