Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts: Current state and future prospects

Hana Raschmanová, Astrid Weninger, Anton Glieder, Karin Kovar, Thomas Vogl

Publikation: Beitrag in einer FachzeitschriftReview eines Fachbereichs (Review article)ForschungBegutachtung

Abstract

Within five years, the CRISPR-Cas system has emerged as the dominating tool for genome engineering, while also changing the speed and efficiency of metabolic engineering in conventional (Saccharomyces cerevisiae and Schizosaccharomyces pombe) and non-conventional (Yarrowia lipolytica, Pichia pastoris syn. Komagataella phaffii, Kluyveromyces lactis, Candida albicans and C. glabrata) yeasts. Especially in S. cerevisiae, an extensive toolbox of advanced CRISPR-related applications has been established, including crisprTFs and gene drives. The comparison of innovative CRISPR-Cas expression strategies in yeasts presented here may also serve as guideline to implement and refine CRISPR-Cas systems for highly efficient genome editing in other eukaryotic organisms.

Originalspracheenglisch
Seiten (von - bis)641-665
Seitenumfang25
FachzeitschriftBiotechnology Advances
Jahrgang36
Ausgabenummer3
DOIs
PublikationsstatusVeröffentlicht - 1 Mai 2018

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CRISPR-Cas Systems
Clustered Regularly Interspaced Short Palindromic Repeats
Yeast
Saccharomyces cerevisiae
Yeasts
Yarrowia
Technology
Kluyveromyces
Metabolic Engineering
Pichia
Schizosaccharomyces
Genes
Candida albicans
Genome
Guidelines
Metabolic engineering
Candida

Schlagwörter

    ASJC Scopus subject areas

    • Biotechnology
    • Bioengineering
    • !!Applied Microbiology and Biotechnology

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    Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts : Current state and future prospects. / Raschmanová, Hana; Weninger, Astrid; Glieder, Anton; Kovar, Karin; Vogl, Thomas.

    in: Biotechnology Advances, Jahrgang 36, Nr. 3, 01.05.2018, S. 641-665.

    Publikation: Beitrag in einer FachzeitschriftReview eines Fachbereichs (Review article)ForschungBegutachtung

    Raschmanová, Hana ; Weninger, Astrid ; Glieder, Anton ; Kovar, Karin ; Vogl, Thomas. / Implementing CRISPR-Cas technologies in conventional and non-conventional yeasts : Current state and future prospects. in: Biotechnology Advances. 2018 ; Jahrgang 36, Nr. 3. S. 641-665.
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    AU - Glieder, Anton

    AU - Kovar, Karin

    AU - Vogl, Thomas

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