Identification of a metagenome-derived esterase with high enantioselectivity in the kinetic resolution of arylaliphatic tertiary alcohols

Robert Kourist, Sajja Hari Krishna, Jesal S Patel, Flash Bartnek, Timothy S Hitchman, David P Weiner, Uwe T Bornscheuer

Publikation: Beitrag in einer FachzeitschriftArtikel

Abstract

35 metagenome-derived esterases bearing a GGG(A)X motif were screened for activity and enantioselectivity in the hydrolysis of a range of tertiary alcohol acetates. Most of the active esterases showed little or no enantioselectivity in the hydrolysis of the terpinyl acetate, linalyl acetate and 3-methylpent-1-yn-3-yl acetate. However, one esterase showed excellent enantioselectivity (E > 100) in the kinetic resolution of 1,1,1-trifluoro-2-phenylbut-3-yn-2-yl acetate as confirmed by a preparative scale reaction.

Originalspracheenglisch
Seiten (von - bis)3310-3313
Seitenumfang4
FachzeitschriftOrganic & biomolecular chemistry
Jahrgang5
Ausgabenummer20
DOIs
PublikationsstatusVeröffentlicht - 2007
Extern publiziertJa

Fields of Expertise

  • Human- & Biotechnology

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