Abstract
The Berberine bridge enzyme-like (BBE-like) protein family are flavoproteins that are present in bacteria, fungi and plants. In Arabidopsis thaliana, seven subgroups have been identified with a total of 27 genes encoding BBE-like enzymes (AtBBE-like). AtBBE-like proteins are involved in stress-induced plant responses as well as developmental processes. Our work focusses on AtBBE-like subgroup six, from which two members have been found to oxidize monolignols. We therefore hypothesize an involvement of AtBBE-like enzymes in the regulation of the extracellular monolignol pool in planta.
In order to unravel spatiotemporal expression patterns of the five genes from AtBBE-like subgroup 6 on the tissue level, we generated transcriptional reporter lines and found expression in roots, where the individual genes show different expression patterns on the tissue level. Additionally, AtBBE-like 15, 24 and 26 reporter lines show expression during flower development. For all genes from subgroup 6, qRT-PCR revealed high levels of expression in roots. Phenotyping of T-DNA insertion lines do not exhibit an obvious phenotype.
Further phenotypic characterization of loss-of-function mutants generated with CRISPR/Cas9, detailed description of reporter lines and generation of fluorescent reporter lines will widen our understanding of subgroup six of the AtBBE-like protein family.
In order to unravel spatiotemporal expression patterns of the five genes from AtBBE-like subgroup 6 on the tissue level, we generated transcriptional reporter lines and found expression in roots, where the individual genes show different expression patterns on the tissue level. Additionally, AtBBE-like 15, 24 and 26 reporter lines show expression during flower development. For all genes from subgroup 6, qRT-PCR revealed high levels of expression in roots. Phenotyping of T-DNA insertion lines do not exhibit an obvious phenotype.
Further phenotypic characterization of loss-of-function mutants generated with CRISPR/Cas9, detailed description of reporter lines and generation of fluorescent reporter lines will widen our understanding of subgroup six of the AtBBE-like protein family.
Titel in Übersetzung | Von Alkohol zu Aldehyd: Charakerisierung von Monolignol Oxidoreductasen und deren mögliche Involvierung in die Regulation des extrazellulären Phenolpools |
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Originalsprache | englisch |
Publikationsstatus | Veröffentlicht - 19 Juli 2022 |
Veranstaltung | 2022 New Phytologist Next Generation Scientist's Meeting - Delta Centre, Tartu, Estland Dauer: 19 Juli 2022 → 22 Juli 2022 https://www.newphytologist.org/nextgenevents |
Konferenz
Konferenz | 2022 New Phytologist Next Generation Scientist's Meeting |
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Land/Gebiet | Estland |
Ort | Tartu |
Zeitraum | 19/07/22 → 22/07/22 |
Internetadresse |
Schlagwörter
- Berberine Bridge Enzymes
- Lignin
- Gene Expression
- Transformation, Genetic
Fields of Expertise
- Human- & Biotechnology