Exploring the interactions of irbesartan and irbesartan–2-hydroxypropyl-β-cyclodextrin complex with model membranes

Adamantia S. Liossi, Dimitrios Ntountaniotis, Tahsin F. Kellici, Maria V. Chatziathanasiadou, Grigorios Megariotis, Maria Mania, Johanna Becker-Baldus, Manfred Kriechbaum, Andraž Krajnc, Eirini Christodoulou, Clemens Glaubitz, Michael Rappolt, Heinz Amenitsch, Gregor Mali, Doros N. Theodorou, Georgia Valsami, Marinos Pitsikalis, Hermis Iatrou, Andreas G. Tzakos, Thomas Mavromoustakos

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

The interactions of irbesartan (IRB) and irbesartan–2-hydroxypropyl-β-cyclodextrin (HP-β-CD) complex with dipalmitoyl phosphatidylcholine (DPPC) bilayers have been explored utilizing an array of biophysical techniques ranging from differential scanning calorimetry (DSC), small angle X-ray scattering (SAXS), ESI mass spectrometry (ESI-MS) and solid state nuclear magnetic resonance (ssNMR). Molecular dynamics (MD) calculations have been also conducted to complement the experimental results. Irbesartan was found to be embedded in the lipid membrane core and to affect the phase transition properties of the DPPC bilayers. SAXS studies revealed that irbesartan alone does not display perfect solvation since some coexisting irbesartan crystallites are present. In its complexed form IRB gets fully solvated in the membranes showing that encapsulation of IRB in HP-β-CD may have beneficial effects in the ADME properties of this drug. MD experiments revealed the topological and orientational integration of irbesartan into the phospholipid bilayer being placed at about 1 nm from the membrane centre.

Originalspracheenglisch
Seiten (von - bis)1089-1098
Seitenumfang10
FachzeitschriftBiochimica et Biophysica Acta / Biomembranes
Jahrgang1859
Ausgabenummer6
DOIs
PublikationsstatusVeröffentlicht - 1 Jun 2017

Schlagwörter

    ASJC Scopus subject areas

    • !!Biophysics
    • !!Biochemistry
    • Zellbiologie

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  • Dieses zitieren

    Liossi, A. S., Ntountaniotis, D., Kellici, T. F., Chatziathanasiadou, M. V., Megariotis, G., Mania, M., ... Mavromoustakos, T. (2017). Exploring the interactions of irbesartan and irbesartan–2-hydroxypropyl-β-cyclodextrin complex with model membranes. Biochimica et Biophysica Acta / Biomembranes, 1859(6), 1089-1098. https://doi.org/10.1016/j.bbamem.2017.03.003