Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members

Aline Telzerow, Juraj Paris, Maria Håkansson, Javier González-Sabín, Nicolas Ríos-Lombardía, Harald Gröger, Francisco Morís, Martin Schürmann, Helmut Schwab*, Kerstin Steiner*

*Korrespondierende/r Autor/in für diese Arbeit

Publikation: Beitrag in einer FachzeitschriftArtikel

Abstract

Amine transaminases (ATAs) are used to synthesize enantiomerically pure amines, which are building blocks for pharmaceuticals and agrochemicals. R-selective ATAs belong to the fold type IV PLP-dependent enzymes, and different sequence-, structure- and substrate scope-based features have been identified in the past decade. However, our knowledge is still restricted due to the limited number of characterized (R)-ATAs, with additional bias towards fungal origin. We aimed to expand the toolbox of (R)-ATAs and contribute to the understanding of this enzyme subfamily. We identified and characterized four new (R)-ATAs. The ATA from Exophiala sideris contains a motif characteristic for d-ATAs, which was previously believed to be a disqualifying factor for (R)-ATA activity. The crystal structure of the ATA from Shinella is the first from a Gram-negative bacterium. The ATAs from Pseudonocardia acaciae and Tetrasphaera japonica are the first characterized (R)-ATAs with a shortened/missing N-terminal helix. The active-site charges vary significantly between the new and known ATAs, correlating with their diverging substrate scope.

Originalspracheenglisch
Seiten (von - bis)1232-1242
Seitenumfang11
FachzeitschriftChemBioChem
Jahrgang22
Ausgabenummer7
Frühes Online-Datum26 Nov 2020
DOIs
PublikationsstatusVeröffentlicht - 6 Apr 2021

ASJC Scopus subject areas

  • !!Biochemistry
  • !!Molecular Medicine
  • !!Molecular Biology
  • Organische Chemie

Fingerprint

Untersuchen Sie die Forschungsthemen von „Expanding the Toolbox of R-Selective Amine Transaminases by Identification and Characterization of New Members“. Zusammen bilden sie einen einzigartigen Fingerprint.

Dieses zitieren