Evaluating the degree of molecular contact between cellulose fiber surfaces using FRET microscopy

Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

Abstract

Abstract: The degree of molecular contact, i.e. the contact area on the nanometer scale, between paper fibers is crucial for the van-der-Waals and hydrogen bond adhesion between the fibers and thus for the fiber-fiber bond strength. We apply Förster resonance energy transfer (FRET) to investigate the degree of contact in the distance range of 1–10 nm between pulp fiber bonds and between thin films. The FRET system with DCCH and FTSC as fluorescence dyes has been validated for spectrophotometry and for local imaging with widefield microscopy, using pHema thin films. Bonding between thin films can be detected with this system, however it has not been possible to achieve a significant FRET signal between bonded pulp fibers. Therefore, we conclude that in principle it is possible to quantify the degree of contact between two surfaces on the nanometer scale with the investigated FRET system. For further work on pulp fibers we recommend an exclusively surface active dyeing, as bulk dyeing massively deteriorates the signal to noise ratio which is likely the reason for the low FRET signal found in this work. Graphic Abstract: [Figure not available: see fulltext.].

Originalspracheenglisch
Seiten (von - bis)7037-7050
FachzeitschriftCellulose
Jahrgang26
Ausgabenummer12
DOIs
PublikationsstatusVeröffentlicht - 1 Jan 2019

Fingerprint

Cellulose
Energy transfer
Microscopic examination
Fibers
Pulp
Dyeing
Thin films
Bond strength (materials)
Spectrophotometry
Signal to noise ratio
Hydrogen bonds
Coloring Agents
Adhesion
Dyes
Fluorescence
Imaging techniques

Schlagwörter

    ASJC Scopus subject areas

    • !!Polymers and Plastics

    Dies zitieren

    Evaluating the degree of molecular contact between cellulose fiber surfaces using FRET microscopy. / Urstöger, Georg; Simoes, Monica G.; Steinegger, Andreas; Schennach, Robert; Hirn, Ulrich.

    in: Cellulose, Jahrgang 26, Nr. 12, 01.01.2019, S. 7037-7050.

    Publikation: Beitrag in einer FachzeitschriftArtikelForschungBegutachtung

    @article{fca449045c1440b2ba4ce57a0c6f3ff9,
    title = "Evaluating the degree of molecular contact between cellulose fiber surfaces using FRET microscopy",
    abstract = "Abstract: The degree of molecular contact, i.e. the contact area on the nanometer scale, between paper fibers is crucial for the van-der-Waals and hydrogen bond adhesion between the fibers and thus for the fiber-fiber bond strength. We apply F{\"o}rster resonance energy transfer (FRET) to investigate the degree of contact in the distance range of 1–10 nm between pulp fiber bonds and between thin films. The FRET system with DCCH and FTSC as fluorescence dyes has been validated for spectrophotometry and for local imaging with widefield microscopy, using pHema thin films. Bonding between thin films can be detected with this system, however it has not been possible to achieve a significant FRET signal between bonded pulp fibers. Therefore, we conclude that in principle it is possible to quantify the degree of contact between two surfaces on the nanometer scale with the investigated FRET system. For further work on pulp fibers we recommend an exclusively surface active dyeing, as bulk dyeing massively deteriorates the signal to noise ratio which is likely the reason for the low FRET signal found in this work. Graphic Abstract: [Figure not available: see fulltext.].",
    keywords = "Adhesion, Cellulose fibers, Contact mechanics, FRET, F{\"o}rster resonance energy transfer",
    author = "Georg Urst{\"o}ger and Simoes, {Monica G.} and Andreas Steinegger and Robert Schennach and Ulrich Hirn",
    year = "2019",
    month = "1",
    day = "1",
    doi = "10.1007/s10570-019-02575-x",
    language = "English",
    volume = "26",
    pages = "7037--7050",
    journal = "Cellulose",
    issn = "0969-0239",
    publisher = "Springer Netherlands",
    number = "12",

    }

    TY - JOUR

    T1 - Evaluating the degree of molecular contact between cellulose fiber surfaces using FRET microscopy

    AU - Urstöger, Georg

    AU - Simoes, Monica G.

    AU - Steinegger, Andreas

    AU - Schennach, Robert

    AU - Hirn, Ulrich

    PY - 2019/1/1

    Y1 - 2019/1/1

    N2 - Abstract: The degree of molecular contact, i.e. the contact area on the nanometer scale, between paper fibers is crucial for the van-der-Waals and hydrogen bond adhesion between the fibers and thus for the fiber-fiber bond strength. We apply Förster resonance energy transfer (FRET) to investigate the degree of contact in the distance range of 1–10 nm between pulp fiber bonds and between thin films. The FRET system with DCCH and FTSC as fluorescence dyes has been validated for spectrophotometry and for local imaging with widefield microscopy, using pHema thin films. Bonding between thin films can be detected with this system, however it has not been possible to achieve a significant FRET signal between bonded pulp fibers. Therefore, we conclude that in principle it is possible to quantify the degree of contact between two surfaces on the nanometer scale with the investigated FRET system. For further work on pulp fibers we recommend an exclusively surface active dyeing, as bulk dyeing massively deteriorates the signal to noise ratio which is likely the reason for the low FRET signal found in this work. Graphic Abstract: [Figure not available: see fulltext.].

    AB - Abstract: The degree of molecular contact, i.e. the contact area on the nanometer scale, between paper fibers is crucial for the van-der-Waals and hydrogen bond adhesion between the fibers and thus for the fiber-fiber bond strength. We apply Förster resonance energy transfer (FRET) to investigate the degree of contact in the distance range of 1–10 nm between pulp fiber bonds and between thin films. The FRET system with DCCH and FTSC as fluorescence dyes has been validated for spectrophotometry and for local imaging with widefield microscopy, using pHema thin films. Bonding between thin films can be detected with this system, however it has not been possible to achieve a significant FRET signal between bonded pulp fibers. Therefore, we conclude that in principle it is possible to quantify the degree of contact between two surfaces on the nanometer scale with the investigated FRET system. For further work on pulp fibers we recommend an exclusively surface active dyeing, as bulk dyeing massively deteriorates the signal to noise ratio which is likely the reason for the low FRET signal found in this work. Graphic Abstract: [Figure not available: see fulltext.].

    KW - Adhesion

    KW - Cellulose fibers

    KW - Contact mechanics

    KW - FRET

    KW - Förster resonance energy transfer

    UR - http://www.scopus.com/inward/record.url?scp=85068206860&partnerID=8YFLogxK

    U2 - 10.1007/s10570-019-02575-x

    DO - 10.1007/s10570-019-02575-x

    M3 - Article

    VL - 26

    SP - 7037

    EP - 7050

    JO - Cellulose

    JF - Cellulose

    SN - 0969-0239

    IS - 12

    ER -